+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

QTL Mapping of Grain Quality Traits Using Introgression Lines Carrying Oryza rufipogon Chromosome Segments in Japonica Rice

QTL Mapping of Grain Quality Traits Using Introgression Lines Carrying Oryza rufipogon Chromosome Segments in Japonica Rice

Rice 9(1): 62

Improved eating quality is a major breeding target in japonica rice due to market demand. Consequently, quantitative trait loci (QTL) for glossiness of cooked rice and amylose content associated with eating quality have received much research focus because of their importance in rice quality. In this study, QTL associated with 12 grain quality traits were identified using 96 introgression lines (IL) of rice developed from an interspecific cross between the Korean elite O. sativa japonica cultivar 'Hwaseong' and O. rufipogon over 7 years. QTL analyses indicated that QTL qDTH6 for heading date, detected on chromosome 6 is associated with variance in grain traits. Most QTLs detected in this study clustered near the qDTH6 locus on chromosome 6, suggesting the effect of qDTH6. O. rufipogon alleles negatively affected grain quality traits except for a few QTLs, including qGCR9 for glossiness of cooked rice on chromosome 9. To characterize the effect of the O. rufipogon locus harboring qGCR9, four lines with a single but different O. rufipogon segment near qGCR9 were compared to Hwaseong. Three lines (O. rufipopgon ILs) having O. rufipogon segment between RM242 and RM245 in common showed higher glossiness of cooked rice than Hwaseong and the other line (Hwaseong IL), indicating that qGCR9 is located in the 3.4-Mb region between RM242 and RM245. Higher glossiness of cooked rice conferred by the O. rufipogon allele might be associated with protein content considering that three lines had lower protein content than Hwaseong (P < 0.1). These three O. rufipogon ILs showed higher yield than Hwaseong and Hwaseong IL due to increase in spikelets per panicle and grain weight indicating the linkage of qGCR9 and yield component QTLs. The qGCR9 locus is of particular interest because of its independence from other undesirable grain quality traits in O. rufipogon. SSR markers linked to qGCR9 can be used to develop high-quality japonica lines and offer a starting point for map-based cloning of genes underlying this trait. To our knowledge, this is the first report to map a beneficial QTL for glossiness of cooked rice from a wild rice, O. rufipogon.

Please choose payment method:

(PDF emailed within 0-6 h: $19.90)

Accession: 058667943

Download citation: RISBibTeXText

PMID: 27882529

DOI: 10.1186/s12284-016-0135-0

Related references

Construction of introgression lines carrying wild rice (Oryza rufipogon Griff.) segments in cultivated rice (Oryza sativa L.) background and characterization of introgressed segments associated with yield-related traits. Tag. Theoretical and Applied Genetics. Theoretische und Angewandte Genetik 112(3): 570-580, 2005

Mapping quantitative trait loci influencing panicle-related traits from Chinese common wild rice Oryza rufipogon using introgression lines. Plant Breeding 128(6): 559-567, 2009

Fine mapping of a grain weight quantitative trait locus on rice chromosome 8 using near-isogenic lines derived from a cross between Oryza sativa and Oryza rufipogon. Tag. Theoretical and Applied Genetics. Theoretische und Angewandte Genetik 113(5): 885-894, 2006

Development and evaluation of chromosome segment substitution lines (CSSLs) carrying chromosome segments derived from Oryza rufipogon in the genetic background of Oryza sativa L. Breeding Science 63(5): 468-475, 2014

Identification of introgressed alien chromosome segments associated with grain quality in Oryza rufipogon x MR219 advanced breeding lines using SSR markers. Genetics and Molecular Research 11(3): 3534-3546, 2013

Development of introgression lines derived from Oryza rufipogon and O. glumaepatula in the genetic background of japonica cultivated rice (O. sativa L.) and evaluation of resistance to rice blast. Breeding Science 60(5): 604-612, 2010

Development of high yielding IR64 x Oryza rufipogon (Griff.) introgression lines and identification of introgressed alien chromosome segments using SSR markers. Euphytica 160(3): 401-409, 2008

Mapping QTL for traits associated with resistance to ferrous iron toxicity in rice (Oryza sativa L.), using japonica chromosome segment substitution lines. Yi Chuan Xue Bao 30(10): 893-898, 2003

Linking root traits and grain yield for rainfed rice in sub-Saharan Africa: Response of Oryza sativa x Oryza glaberrima introgression lines under drought. Field Crops Research 165: 25-35, 2014

Construction of introgression lines of Oryza rufipogon and evaluation of important agronomic traits. Tag. Theoretical and Applied Genetics. Theoretische und Angewandte Genetik 2018, 2018

Identification of interspecific heterotic loci associated with agronomic traits in rice introgression lines carrying genomic fragments of Oryza glaberrima. Euphytica 213(8): 176, 2017

Molecular mapping of grain physico-chemical and cooking quality traits using recombinant inbred lines in rice (Oryza sativaL.). Journal of Plant Biochemistry and Biotechnology 21(1): 1-10, 2012

Further QTL mapping for yield component traits using introgression lines in rice (Oryza sativa L.) under drought field environments. Euphytica 214(2): 33, 2018

Construction of chromosome segment substitution lines carrying overlapping chromosome segments of the whole wild rice genome and identification of quantitative trait loci for rice quality. Zhi Wu Sheng Li Yu Fen Zi Sheng Wu Xue Xue Bao 32(3): 354-362, 2006

Transgressive Variation for Yield Components Measured throughout the Growth Cycle of Jefferson Rice (Oryza sativa) x O-rufipogon Introgression Lines. Crop Science 56(5): 2336-2347, 2016