EurekaMag
+ Translate
+ Most Popular
Gaucher's disease;thirty-two years experience at Siriraj Hospital
A study of Macrobathra Meyrick from China (Lepidoptera, Cosmopterigidae)
First occurrence in ores of tetragonal chalcocite
Effects of trace element nutrition on sleep patterns in adult women
N.Z. range management guidelines. 2. Design of grazing management systems for tussock country
A case of lipoma of the esophagus
A revision of world Acanthosomatidae (Heteroptera: Pentatomidae): keys to and descriptions of subfamilies, tribes and genera, with designation of types
Life history of the coronate scyphozoan Linuche unguiculata (Swartz, 1788)
Perceptual restoration of obliterated sounds
Mutagenicity studies on two chromium(III) coordination compounds
The formation of the skeleton. I. Growth of a long bone. 1st appearance of a center of calcification
Leucopenia and abnormal liver function in travellers on malaria chemoprophylaxis
The joint commission: four key root causes loom large in sentinel event data
Treatment of vitiligo with topical 15% lactic acid solution in combination with ultra violet-A
Behaviour of dairy cows within three hours after feed supply: I. Influence of housing type and time elapsing after feed supply
Observations of the propagation velocity and formation mechanism of burst fractures caused by gunshot
Management and control of patients with type 2 diabetes mellitus in Lebanon: results from the International Diabetes Management Practices Study (IDMPS)
The diet composition and nutritional knowledge of patients with anorexia nervosa
Physoporella croatica Herak, 1958 of the Slovak karst Anisian (Slovakia, the West Carpathians Mts.)
Bright lights, big noise. How effective are vehicle warning systems?
Ein Plesiosaurier-Rest mit Magensteinen aus mittlerem Lias von Quedlinburg
Incidence of Chlamydia trachomatis in patients with sterility
Monster soup: the microscope and Victorian fantasy
Preliminary tests with residual sprays against poultry lice
Duration of the life of plants in phylogeny

RNA-seq analysis of the gonadal transcriptome during Alligator mississippiensis temperature-dependent sex determination and differentiation


RNA-seq analysis of the gonadal transcriptome during Alligator mississippiensis temperature-dependent sex determination and differentiation



Bmc Genomics 17: 77



ISSN/ISBN: 1471-2164

PMID: 26810479

DOI: 10.1186/s12864-016-2396-9

The American alligator (Alligator mississippiensis) displays temperature-dependent sex determination (TSD), in which incubation temperature during embryonic development determines the sexual fate of the individual. However, the molecular mechanisms governing this process remain a mystery, including the influence of initial environmental temperature on the comprehensive gonadal gene expression patterns occurring during TSD. Our characterization of transcriptomes during alligator TSD allowed us to identify novel candidate genes involved in TSD initiation. High-throughput RNA sequencing (RNA-seq) was performed on gonads collected from A. mississippiensis embryos incubated at both a male and a female producing temperature (33.5 °C and 30 °C, respectively) in a time series during sexual development. RNA-seq yielded 375.2 million paired-end reads, which were mapped and assembled, and used to characterize differential gene expression. Changes in the transcriptome occurring as a function of both development and sexual differentiation were extensively profiled. Forty-one differentially expressed genes were detected in response to incubation at male producing temperature, and included genes such as Wnt signaling factor WNT11, histone demethylase KDM6B, and transcription factor C/EBPA. Furthermore, comparative analysis of development- and sex-dependent differential gene expression revealed 230 candidate genes involved in alligator sex determination and differentiation, and early details of the suspected male-fate commitment were profiled. We also discovered sexually dimorphic expression of uncharacterized ncRNAs and other novel elements, such as unique expression patterns of HEMGN and ARX. Twenty-five of the differentially expressed genes identified in our analysis were putative transcriptional regulators, among which were MYBL2, MYCL, and HOXC10, in addition to conventional sex differentiation genes such as SOX9, and FOXL2. Inferred gene regulatory network was constructed, and the gene-gene and temperature-gene interactions were predicted. Gonadal global gene expression kinetics during sex determination has been extensively profiled for the first time in a TSD species. These findings provide insights into the genetic framework underlying TSD, and expand our current understanding of the developmental fate pathways during vertebrate sex determination.

Please choose payment method:






(PDF emailed within 0-6 h: $19.90)

Accession: 058681988

Download citation: RISBibTeXText

Related references

Gonadal sex differentiation in Alligator mississippiensis, a species with temperature-dependent sex determination. Cell and Tissue Research 273(1): 149-162, 1993

Cloning and expression analysis of two ZFY-related zinc finger genes from Alligator mississippiensis, a species with temperature-dependent sex determination. Gene 119(2): 221-228, 1992

Temperature-dependent sex determination in Alligator mississippiensis. Journal of Zoology 200: 3-77, 1983

Gonadal development and differentiation in alligator mississippiensis at male and female producing incubation temperatures. Journal of Zoology (London) 218(4): 679-688, 1989

Temperature dependent differentiation of male and female alligator embryos differences in protein synthesis in embryonic gonadal tissues. Journal of Cell Biology 115(3 Part 2): 146A, 1991

Influences of sex, incubation temperature, and environmental quality on gonadal estrogen and androgen receptor messenger RNA expression in juvenile American alligators (Alligator mississippiensis). Biology of reproduction 82(1): 194-201, 2010

Transcriptome sequencing and comparative analysis of adult ovary and testis identify potential gonadal maintenance-related genes in Mauremys reevesii with temperature-dependent sex determination. Peerj 7: E6557, 2019

Effect of temperature on central chemical control of ventilation in the alligator Alligator mississippiensis. Journal of Experimental Biology 179: 261-272, 1993

The effect of body temperature on ventilatory sensitivity to carbon di oxide in the alligator alligator mississippiensis. Physiologist 24(4): 129, 1981

Effect of temperature on the rates of digestion amino acid absorption and assimilation in the alligator alligator mississippiensis. Comparative Biochemistry and Physiology A 83(3): 585-588, 1986

Metabolic heating of embryos and sex determination in the American Alligator, Alligator mississippiensis. Journal of Thermal Biology 28(2): 159-165, 2003

The cloning and expression analysis of Lhx9 during gonadal sex differentiation in the red-eared slider turtle, Trachemys scripta, a species with temperature-dependent sex determination. Journal of Experimental Zoology. Part B Molecular and Developmental Evolution 320(4): 238-246, 2013

Isolation and determination of the primary structure of a lectin protein from the serum of the American alligator (Alligator mississippiensis). Comparative Biochemistry and Physiology. Part B Biochemistry and Molecular Biology 161(2): 161-169, 2012

Body temperature null distributions in reptiles with nonzero heat capacity: seasonal thermoregulation in the American alligator (Alligator mississippiensis). Physiological and biochemical zoology: PBZ 76(3): 348-359, 2003

Determination Of Selected Metals In Alligator ( Alligator mississippiensis ) Tissues By Inductively Coupled Plasma-Optical Emission Spectrometry. Instrumentation Science and Technology 39(4): 368-373, 2011