EurekaMag
+ Translate
+ Most Popular
Cunninghamia lanceolata plantations in China
Mammalian lairs in paleo ecological studies and palynology
Studies on technological possibilities in utilization of anhydrous milk fat for production of recombined butter-like products
Should right-sided fibroelastomas be operated upon?
Large esophageal lipoma
Apoptosis in the mammalian thymus during normal histogenesis and under various in vitro and in vivo experimental conditions
Poissons characoides nouveaux ou non signales de l'Ilha do Bananal, Bresil
Desensitizing efficacy of Colgate Sensitive Maximum Strength and Fresh Mint Sensodyne dentifrices
Administration of fluid by subcutaneous infusion: revival of a forgotten method
Tundra mosquito control - an impossible dream?
Schizophrenia for primary care providers: how to contribute to the care of a vulnerable patient population
Geochemical pattern analysis; method of describing the Southeastern limestone regional aquifer system
Incidence of low birth weights in a hospital of Mexico City
Tabanidae
Graded management intensity of grassland systems for enhancing floristic diversity
Microbiology and biochemistry of cheese and fermented milk
The ember tetra: a new pygmy characid tetra from the Rio das Mortes, Brazil, Hyphessobrycon amandae sp. n. (Pisces, Characoidei)
Risk factors of contrast-induced nephropathy in patients after coronary artery intervention
Renovation of onsite domestic wastewater in a poorly drained soil
Observations of the propagation velocity and formation mechanism of burst fractures caused by gunshot
Systolic blood pressure in a population of infants in the first year of life: the Brompton study
Haematological studies in rats fed with metanil yellow
Studies on pasteurellosis. I. A new species of Pasteurella encountered in chronic fowl cholera
Dormancy breaking and germination of Acacia salicina Lindl. seeds
therapy of lupus nephritis. a two-year prospective study

Rapid Diagnosis of Soybean Seedling Blight Caused by Rhizoctonia solani and Soybean Charcoal Rot Caused by Macrophomina phaseolina Using LAMP Assays


Rapid Diagnosis of Soybean Seedling Blight Caused by Rhizoctonia solani and Soybean Charcoal Rot Caused by Macrophomina phaseolina Using LAMP Assays



Phytopathology 105(12): 1612-1617



ISSN/ISBN: 0031-949X

PMID: 26606587

DOI: 10.1094/phyto-01-15-0023-r

A new method of direct detection of pathogenic fungi in infected soybean tissues has been reported here. The method rapidly diagnoses soybean seedling blight caused by Rhizoctonia solani and soybean charcoal rot caused by Macrophomina phaseolina, and features loop-mediated isothermal amplification (LAMP). The primers were designed and screened using internal transcribed spacers (ITS) as target DNAs of both pathogens. An ITS-Rs-LAMP assay for R. solani and an ITS-Mp-LAMP assay for M. phaseolina that can detect the pathogen in diseased soybean tissues in the field have been developed. Both LAMP assays efficiently amplified the target genes over 60 min at 62°C. A yellow-green color (visible to the naked eye) or intense green fluorescence (visible under ultraviolet light) was only observed in the presence of R. solani or M. phaseolina after addition of SYBR Green I. The detection limit of the ITS-Rs-LAMP assay was 10 pg μl⁻¹ of genomic DNA; and that of the ITS-Mp-LAMP assay was 100 pg μl⁻¹ of genomic DNA. Using the two assays described here, we successfully and rapidly diagnosed suspect diseased soybean samples collected in the field from Jiangsu and Anhui provinces.

Please choose payment method:






(PDF emailed within 0-6 h: $19.90)

Accession: 058690657

Download citation: RISBibTeXText

Related references

First Report of Charcoal Rot Caused by Macrophomina phaseolina in Soybean in New York. Plant Disease 97(11): 1506, 2013

First report of charcoal rot on soybean caused by Macrophomina phaseolina in North Dakota. Plant Disease 87(5): 601, 2003

First Report of Charcoal Rot on Soybean Caused by Macrophomina phaseolina in North Dakota. Plant Disease 87(5): 601, 2003

Studies on charcoal rot of soybean caused by Macrophomina phaseolina (Tassi) Goid. Pakistan Journal of Phytopathology 2(1-2): 22-30, 1990

First Report of Charcoal Rot Epidemics Caused by Macrophomina phaseolina in Soybean in Iowa. Plant Disease 89(5): 526, 2005

First report of charcoal rot epidemics caused by Macrophomina phaseolina in soybean in Iowa. Plant Disease 89(5): 526, 2005

Supression of charcoal rot in soybean caused by Macrophomina phaseolina using secondary nutrients. Phytopathology 105(4), 2015

Effects of Clonostachys species on charcoal rot disease caused by Macrophomina phaseolina in soybean. Phytopathology 106(12): 53-54, 2016

Rhizobium japonicum as a Biocontrol Agent of Soybean Root Rot Disease Caused by Fusarium solani and Macrophomina phaseolina. Plant Protection Science 48(4): 149-155, 2012

Seedling infection of soybean by isolates of rhizoctonia solani ag 1 causal agent of aerial blight and web blight of soybean. Plant Disease 74(7): 485-488, 1990

Studies on the soybean blight caused by Rhizoctonia solani. Indian Journal of Mycology and Plant Pathology 4(1): 101-103, 1974

Aerial blight of soybean (Glycine max) caused by Rhizoctonia solani. Indian Journal of Agricultural Sciences 68(5): 277-278, 1998

Severity of the foliar blight of the soybean caused by Rhizoctonia solani AG-1 IA in function of doses of potassium. Summa Phytopathologica 33(1): 56-62, 2007

A technique for testing soybean cultivars for resistance to aerial web blight disease caused by Rhizoctonia solani. Journal of the Mississippi Academy of Sciences: 442, 1989

Seedling blight of jute caused by macrophomina phaseolina and its control. Indian Journal of Mycology and Plant Pathology 11(1): 40-45, 1981