Relationship between Single Nucleotide Polymorphism of Interferon-gamma Gene +874 T/A and Efficacy of Immunosuppressive Therapy in Patients with Aplastic Anemia

Zeng, Q.; Wang, H.; Chang, H.; Liu, T.

Zhongguo Shi Yan Xue Ye Xue Za Zhi 23(5): 1375-1379

2015


ISSN/ISBN: 1009-2137
PMID: 26524041
Accession: 058729445

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Abstract
To investigate whether the single nucleotide polymorphism (SNP) of interferon-gamma (interferon-γ + 874 A/T correlates with aplastic anemia (AA) and its efficacy of immunosuppressive therapy. The blood samples were collected from 133 AA patients in West China Hospital, out of them 62 AA patients received immunosuppressive therapy and were observed for more than 4 months. A total of 100 healthy adults were selected as control. The polymerase chain reation (PCR), agarose gel electrophoresis and DNA sequencing were performed for detecting the polymorphism gene fragment of IFN-γ + 874 A/T in AA patients and controls. (1) The frequences of IFN-γ + 874 TT genotype and T allele in AA group were 24.8% and 48.9% respectively and were significantly higher than those in control group 7.0% and 23.0% (P < 0.001, P < 0.001); (2) In 62 AA patients, 34 AA patients achived response to immunosuppressive therapy, among them the response rates of patients with IFN-γ + 874A/T and patients with T allele were 75% and 71.4%, and were significantly higher than those of patients with AA genotype and A allele (27.3% and 41.2%) (P = 0.008, P = 0.001). The IFN-γ + 874A/T gene polymorphism not only correlates with the susceptibility to aplastic anemia, but also with the efficacy of immuno-suppressive therapy.