+ Site Statistics
+ Search Articles
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ PDF Full Text
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Translate
+ Recently Requested

Role of the yeast DNA repair protein Nej1 in end processing during the repair of DNA double strand breaks by non-homologous end joining

Role of the yeast DNA repair protein Nej1 in end processing during the repair of DNA double strand breaks by non-homologous end joining

Dna Repair 31: 1-10

DNA double strand breaks (DSB)s often require end processing prior to joining during their repair by non-homologous end joining (NHEJ). Although the yeast proteins, Pol4, a Pol X family DNA polymerase, and Rad27, a nuclease, participate in the end processing reactions of NHEJ, the mechanisms underlying the recruitment of these factors to DSBs are not known. Here we demonstrate that Nej1, a NHEJ factor that interacts with and modulates the activity of the NHEJ DNA ligase complex (Dnl4/Lif1), physically and functionally interacts with both Pol4 and Rad27. Notably, Nej1 and Dnl4/Lif1, which also interacts with both Pol4 and Rad27, independently recruit the end processing factors to in vivo DSBs via mechanisms that are additive rather than redundant. As was observed with Dnl4/Lif1, the activities of both Pol4 and Rad27 were enhanced by the interaction with Nej1. Furthermore, Nej1 increased the joining of incompatible DNA ends in reconstituted reactions containing Pol4, Rad27 and Dnl4/Lif1, indicating that the stimulatory activities of Nej1 and Dnl4/Lif1 are also additive. Together our results reveal novel roles for Nej1 in the recruitment of Pol4 and Rad27 to in vivo DSBs and the coordination of the end processing and ligation reactions of NHEJ.

(PDF emailed within 0-6 h: $19.90)

Accession: 058791549

Download citation: RISBibTeXText

PMID: 25942368

DOI: 10.1016/j.dnarep.2015.04.003

Related references

Involvement of p53 in the repair of DNA double strand breaks: multifaceted Roles of p53 in homologous recombination repair (HRR) and non-homologous end joining (NHEJ). Sub-Cellular Biochemistry 85: 321-336, 2014

The role of nonhomologous DNA end joining, conservative homologous recombination, and single-strand annealing in the cell cycle-dependent repair of DNA double-strand breaks induced by H(2)O(2) in mammalian cells. Radiation Research 170(6): 784-793, 2009

Repair of DNA double strand breaks by non-homologous end joining. Biochimie 85(11): 1161-1173, 2004

Role of yeast SIR genes and mating type in directing DNA double-strand breaks to homologous and non-homologous repair paths. Current Biology 9(14): 767-770, July 15, 1999

Double-Strand Breaks Repair by Non-Homologous DNA End Joining in Mammalian Cells. Current Genomics 7(5): 311-322, 2006

The non-homologous end-joining factor Nej1 inhibits resection mediated by Dna2-Sgs1 nuclease-helicase at DNA double strand breaks. Journal of Biological Chemistry 292(35): 14576-14586, 2017

The effect of stochasticity on repair of DNA double strand breaks throughout non-homologous end joining pathway. Mathematical Medicine and Biology 35(4): 517-539, 2017

Repair of ionizing radiation-induced DNA double-strand breaks by non-homologous end-joining. Biochemical Journal 417(3): 639-650, 2009

Repair of DNA double-strand breaks by XRCC4-dependent and -independent non-homologous end-joining. International Journal of Radiation Oncology Biology Physics 54(2 Supplement): 51, 2002

DNA double strand breaks induced by the indirect effect of radiation are more efficiently repaired by non-homologous end joining compared to homologous recombination repair. Mutation Research 756(1-2): 21-29, 2013

Either non-homologous ends joining or homologous recombination is required to repair double-strand breaks in the genome of macrophage-internalized Mycobacterium tuberculosis. Plos One 9(3): E92799, 2014

The non-homologous end-joining (NHEJ) pathway for the repair of DNA double-strand breaks: I. A mathematical model. Radiation Research 179(5): 530-539, 2013

Induction and repair of DNA double strand breaks: the increasing spectrum of non-homologous end joining pathways. Mutation Research 711(1-2): 61-72, 2011

Repair of DNA double-strand breaks in mammalian cells by homologous recombination and end-joining mechanisms. Journal of Cellular Biochemistry Supplement 0(21A): 328, 1995

Ku protein stimulates DNA end joining by mammalian DNA ligases: a direct role for Ku in repair of DNA double-strand breaks. Embo Journal 17(2): 609-614, 1998