+ Site Statistics
References:
54,258,434
Abstracts:
29,560,870
PMIDs:
28,072,757
+ Search Articles
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ PDF Full Text
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Translate
+ Recently Requested

Role of vitamin D 3 combined to alginates in preventing acid and oxidative injury in cultured gastric epithelial cells



Role of vitamin D 3 combined to alginates in preventing acid and oxidative injury in cultured gastric epithelial cells



Bmc Gastroenterology 16(1): 127



Gastric diseases are a worldwide problem in modern society, as reported in the USA, in the range of 0.5-2 episodes/year/person and an incidence of 5-100 episodes/1000/week according to seasons and age. There is convincing evidence that oxidative stress is involved in the pathogenesis of acute gastric injury. Acid secreted from gastric parietal cells determines mucosal injuries which in turn cause inflammation and oxidative stress. Consequent inflammation produces free radicals by mitochondria thus causing lipid peroxidation, oxidative and acidic stress, which can lead to cell apoptosis. Vitamin D3, the active form of vitamin D, may counteract intracellular cell death and improve epithelial regeneration. This study was planned to assess whether vitamin D3 is a protective factor against acid injury and oxidative stress in gastric epithelial cells. Primary epithelial cells and GTL-16 cells have been used to test the effects of Grisù® alone or in combination with vitamin D3 during oxidative stress or high acid exposition measuring cell viability, ROS production, cellular adhesion time along with apoptotic, autophagic and survival pathways. The combined effect of Grisù® and vitamin D3 was found more effective in counteracting the negative consequences of oxidative stress and acidity conditions than some other gastroprotective agents, such as Maalox® or Gaviscon®. In case of oxidative stress or acidity condition the stimulation with Grisù® alone caused an improvement of cell viability and a reduction of ROS production on epithelial gastric cells. In addition, the adhesion time of the cells was improved. All these effects were increased by the presence of vitamin D3. Similar data were also observed in primary gastric epithelial cells confirming the results obtained in GTL-16 cells. These results suggest that Grisù® in combination with vitamin D3 may exert a gastroprotective effect to maintain or restore the integrity of gastric epithelium through an antioxidant pathway, inhibiting apoptosis and activating survival kinases. Moreover, the combination of Grisù® and vitamin D3 improves cell viability and decreases ROS production compared to other gastroprotective agents combined with vitamin D3. All these data were validated using primary cells isolated from gastric tissue.

(PDF emailed within 0-6 h: $19.90)

Accession: 058791824

Download citation: RISBibTeXText

PMID: 27717330

DOI: 10.1186/s12876-016-0543-z


Related references

Novel role of vitamin k in preventing oxidative injury to developing oligodendrocytes and neurons. Journal of Neuroscience 23(13): 5816-5826, 2003

Novel role of vitamin K in preventing oxidative injury in developing oligodendrocytes and immature neurons. Society for Neuroscience Abstracts 27(1): 879, 2001

The effects of alpha lipoic acid in preventing oxidative stress-induced retinal pigment epithelial cell injury. Canadian Journal of Physiology and Pharmacology 92(9): 765-772, 2015

Acid exposure stimulates E-cadherin expression on rat cultured gastric epithelial cells in order to stabilize epithelial permeability probably through influx of calcium. Gastroenterology 118(4 Suppl 2 Part 2): AGA A1277, April, 2000

H2O2 oxidative damage in cultured oral epithelial cells: the effect of short-term vitamin C exposure. Anticancer Research 21(4a): 2719-2724, 2001

Exogenous glutathione effects cultured human retinal pigment epithelial cells from oxidative injury. Investigative Ophthalmology & Visual Science 28(3 SUPPL): 255, 1987

Oxidative stress increases gene expression of heparin-binding EGF-like growth factor and amphiregulin in cultured rat gastric epithelial cells. Biochemical and Biophysical Research Communications 226(2): 542-546, 1996

DNA damage and oxidative stress induced by Helicobacter pylori in gastric epithelial cells: protection by vitamin C and sodium selenite. Die Pharmazie 61(7): 631-637, 2006

Mitochondrial phospholipid hydroperoxide glutathione peroxidase plays a major role in preventing oxidative injury to cells. Journal of Biological Chemistry 274(8): 4924-4933, 1999

A study of the chain stiffness and extension of alginates, in vitro epimerized alginates, and periodate-oxidized alginates using size-exclusion chromatography combined with light scattering and viscosity detectors. Biomacromolecules 7(7): 2136-2146, 2006

Role of (Ca-2+)-i in lethal oxidative injury in rat cultured inner medullary collecting duct cells. Pfluegers Archiv European Journal of Physiology 430(5): 697-704, 1995

Role of [Ca2+]i in lethal oxidative injury in rat cultured inner medullary collecting duct cells. Pflugers Archiv 430(5): 697-704, 1995

A study of the chain stiffness and extension of alginates, in vitro epimerized alginates, and periodate-oxidized alginates using size-exclusion chromatography combined with light scattering and viscosity detectors (vol 7, pg 2136, 2006). Biomacromolecules 8(8): 2627, 2007

Interleukin-8 stimulates leukocyte migration across a monolayer of cultured rabbit gastric epithelial cells: Effect associated with the impairment of gastric epithelial barrier function. Digestive Diseases and Sciences 42(6): 1210-1215, 1997