+ Site Statistics
References:
54,258,434
Abstracts:
29,560,870
PMIDs:
28,072,757
+ Search Articles
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ PDF Full Text
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Translate
+ Recently Requested

Roles of 5-HT 1A receptor in the expression of AMPA receptor and BDNF in developing mouse cortical neurons



Roles of 5-HT 1A receptor in the expression of AMPA receptor and BDNF in developing mouse cortical neurons



Neuroscience Research 115: 13-20



The possible interactions between serotonergic and glutamatergic systems during neural development and under the pathogenesis of depression remain unclear. We now investigated roles of 5-HT1A receptor in the mRNA expression of AMPA receptor subunits (GluR1 and GluR2) and brain-derived neurotrophic factor (BDNF) using primary culture of cerebral cortex of mouse embryos. Neurons at embryonic day 18 were cultured for 3days or 14days and then treated with 5-HT1A receptor agonist (8-OH-DPAT) for 3h or 24h. In neurons cultured for 3 days, 8-OH-DPAT treatment for both 3h and 24h increased the mRNA levels of BDNF and GluR1, but not GluR2. In neurons cultured for 14 days, however, 8-OH-DPAT had no effects on these mRNA levels. Next, we examined in vivo roles of 5-HT1A receptor by administration of 8-OH-DPAT to newborn mice. Twenty-four hours after the oral administration of 8-OH-DPAT, the mRNA expression of BDNF was decreased in the frontal cortex, but had no effects on the mRNA expression of GluR1 and GluR2. Taken together, the present study suggests that 5-HT1A receptor activation modulates mRNA expression of AMPA receptor subunit and BDNF in cortical neurons, and the effects are different between in vitro and in vivo.

(PDF emailed within 0-6 h: $19.90)

Accession: 058791991

Download citation: RISBibTeXText

PMID: 27702575

DOI: 10.1016/j.neures.2016.09.008


Related references

Brain-derived neurotrophic factor signal enhances and maintains the expression of AMPA receptor-associated PDZ proteins in developing cortical neurons. Developmental Biology 263(2): 6-30, 2003

Effects of sodium arsenite on neurite outgrowth and glutamate AMPA receptor expression in mouse cortical neurons. Neurotoxicology 37: 197-206, 2014

Distinct roles of GluA2-lacking AMPA receptor expression in dopamine D1 or D2 receptor neurons in animal behavior. Neuroscience 2018, 2018

Metabotropic glutamate receptor 2 and corticotrophin-releasing factor receptor-1 gene expression is differently regulated by BDNF in rat primary cortical neurons. Synapse 67(11): 794-800, 2014

β3 integrin interacts directly with GluA2 AMPA receptor subunit and regulates AMPA receptor expression in hippocampal neurons. Proceedings of the National Academy of Sciences of the United States of America 109(4): 1323-1328, 2012

Beta3 integrin interacts directly with GluA2 AMPA receptor subunit and regulates AMPA receptor expression in hippocampal neurons. 2012

BDNF contributes to both rapid and homeostatic alterations in AMPA receptor surface expression in nucleus accumbens medium spiny neurons. European Journal of Neuroscience 39(7): 1159-1169, 2014

Expression of BDNF receptor TrkBmRNA in hypoxia-induced fetal cortical neurons. Journal of West China University of Medical Sciences 33(4): 573-576, October, 2002

Activation of adenosine A2A receptor up-regulates BDNF expression in rat primary cortical neurons. Neurochemical Research 36(12): 2259-2269, 2012

Combinations of AMPA receptor subunit expression in individual cortical neurons correlate with expression of specific calcium-binding proteins. Journal of Neuroscience 17(5): 1570-1581, 1997

Oroxylin A Induces BDNF Expression on Cortical Neurons through Adenosine A2A Receptor Stimulation: A Possible Role in Neuroprotection. Biomolecules and Therapeutics 20(1): 27-35, 2012

Group II metabotropic glutamate receptor agonist LY379268 regulates AMPA receptor trafficking in prefrontal cortical neurons. Plos One 8(4): E61787, 2013

Expression of AMPA GluR1 receptor subunits modulates dendritic growth in developing motor neurons. Society for Neuroscience Abstracts 26(1-2): Abstract No -315 11, 2000

Substance P receptor activation induces downregulation of the AMPA receptor functionality in cortical neurons from a genetic model of Amyotrophic Lateral Sclerosis. Neurobiology of Disease 44(1): 92-101, 2011

Insulin can induce the expression of a memory-related synaptic protein through facilitating AMPA receptor endocytosis in rat cortical neurons. Cellular and Molecular Life Sciences 71(20): 4069-4080, 2014