+ Site Statistics
References:
54,258,434
Abstracts:
29,560,870
PMIDs:
28,072,757
+ Search Articles
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ PDF Full Text
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Translate
+ Recently Requested

Roles of the Endoplasmic Reticulum Stress Transducer OASIS in Ossification of the Posterior Longitudinal Ligament



Roles of the Endoplasmic Reticulum Stress Transducer OASIS in Ossification of the Posterior Longitudinal Ligament



Clinical Spine Surgery (): -



Vitro molecular research on the posterior longitudinal ligament fibroblasts. To investigate different expression of OASIS between spinal ligament fibroblasts from the patients with ossification of the posterior longitudinal ligament (OPLL) and non-OPLL patients and demonstrate knockdown of OASIS protein expression by RNA interference inhibiting expression of type I collagen in OPLL cells. OPLL is characterized by ectopic bone formation in spinal ligaments. Some evidence indicates that ligament fibroblasts from OPLL patients have osteogenic characteristics. However, the relevant intracellular signaling pathways remain unclear. Spinal ligament cells were cultured using tissue fragment cell culture and identified via immunocytochemistry and immunofluorescence. The messenger RNA expression of osteoblast-specific genes of osteocalcin (OCN), alkaline phosphatase (ALP), and type I collagen (COL I) were detected in OPLL and non-OPLL cells by semiquantitative reverse transcription-polymerase chain reaction. The protein expression of OASIS was detected via Western blotting. And then, after 72 hours, when RNA interference against OASIS was performed in OPLL cells, expression of the osteoblast-specific genes was compared again between the transfection group and the nontransfection group. Spinal ligament fibroblasts were observed 7 to 10 days after cell culture. Immunocytochemistry and immunofluorescence exhibited positive results of vimentin staining. The messenger RNA expressions of OCN, ALP and COL I and protein expressions of OASIS from OPLL cells were significantly greater than those from non-OPLL cells. In addition, knockdown of OASIS protein expression inhibited the messenger RNA expressions of COL I remarkably in the transfection group compared with the nontransfection group, at 72 hours after RNA interference targeting OASIS was performed in OPLL cells. The cultured fibroblasts from OPLL patients exhibited osteogenic characteristics, and OASIS expression plays an important role in the development of OPLL through the expression of type I collagen.

(PDF emailed within 0-6 h: $19.90)

Accession: 058792532

Download citation: RISBibTeXText

PMID: 27171659

DOI: 10.1097/BSD.0b013e3182908c21


Related references

Roles of the endoplasmic reticulum stress transducer OASIS in fracture healing. Bone 49(4): 724-732, 2011

A radiographic study of the progression of ossification of the cervical posterior longitudinal ligament: the correlation between the ossification of the posterior longitudinal ligament and that of the anterior longitudinal ligament. Nihon Seikeigeka Gakkai Zasshi 68(9): 725-736, 1994

Does ossification of the posterior longitudinal ligament progress after laminoplasty? Radiographic and clinical evidence of ossification of the posterior longitudinal ligament lesion growth and the risk factors for late neurologic deterioration. Journal of Neurosurgery. Spine 17(6): 512-524, 2013

Updates on ossification of posterior longitudinal ligament. Ossification front of posterior longitudinal ligament and cellular biological assessment of chronic mechanical compressed spinal cord. Clinical Calcium 19(10): 1472-1479, 2009

Updates on ossification of posterior longitudinal ligament. Surgical outcome of anterior decompression and fusion for the ossification of posterior longitudinal ligament of the cervical spine : a comparison with laminoplasty. Clinical Calcium 19(10): 1486-1492, 2009

Updates on ossification of posterior longitudinal ligament. Genetic approach to the susceptibility genes for ossification of posterior longitudinal ligament of the spine (OPLL) and for its molecular pathogenesis. Clinical Calcium 19(10): 1457-1461, 2009

Updates of ossification of posterior longitudinal ligament. Clinical results and complication of surgery for thoracic myelopathy due to ossification of posterior longitudinal ligament. Clinical Calcium 19(10): 1499-1504, 2009

OASIS, an endoplasmic reticulum stress transducer, is involved in normal bone formation. 2007

Updates on ossification on posterior longitudinal ligament. Electrophysiological diagnosis of spinal cord dysfunction in ossification of posterior longitudinal ligament. Clinical Calcium 19(10): 1435-1440, 2009

Physiological functions of endoplasmic reticulum stress transducer OASIS in central nervous system. Anatomical Science International 89(1): 11-20, 2014

Transcriptional regulation of VEGFA by the endoplasmic reticulum stress transducer OASIS in ARPE-19 cells. Plos One 8(1): E55155-E55155, 2013

The endoplasmic reticulum stress transducer OASIS is involved in the terminal differentiation of goblet cells in the large intestine. Journal of Biological Chemistry 287(11): 8144-8153, 2012

Laminectomy with posterior wiring and fusion for cervical ossification of the posterior longitudinal ligament, spondylosis, ossification of the yellow ligament, stenosis, and instability: a study of 5 patients. Journal of Spinal Disorders 12(6): 461-466, 1999

Updates on ossification of posterior longitudinal ligament. Image diagnosis of ossification of posterior longitudinal ligament and associated diseases. Clinical Calcium 19(10): 1426-1434, 2009

Characteristics of ossification of the spinal ligament; incidence of ossification of the ligamentum flavum in patients with cervical ossification of the posterior longitudinal ligament - Analysis of the whole spine using multidetector CT. Journal of Orthopaedic Science 21(4): 439-445, 2016