+ Site Statistics
References:
54,258,434
Abstracts:
29,560,870
PMIDs:
28,072,757
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

SOD3 Ameliorates H2O2-Induced Oxidative Damage in SH-SY5Y Cells by Inhibiting the Mitochondrial Pathway



SOD3 Ameliorates H2O2-Induced Oxidative Damage in SH-SY5Y Cells by Inhibiting the Mitochondrial Pathway



Neurochemical Research 41(7): 1818-1830



This study was designed to investigate the protective effects of extracellular superoxide dismutase (SOD3) against hydrogen peroxide (H2O2) induced damage in human neuroblastoma SH-SY5Y cells and to elucidate the mechanisms responsible for this beneficial effect. SOD3-overexpressing SH-SY5Y cells were generated by adenoviral vector-mediated infection, and H2O2 was then added into the cell culture system to establish an in vitro model of oxidative stress. Cell viability, the generation of intracellular reactive oxygen species (ROS), the expression and activity of antioxidant enzymes, the levels of lipid peroxidation malondialdehyde (MDA), the expression of mitochondrial apoptosis-related genes, and calcium imaging were examined. Following H2O2 exposure, the over-expression of SOD3 promoted the survival of SH-SY5Y cells; decreased the production of ROS, MDA levels, cytochrome C, caspase-3, caspase-9, and Bax gene expression, and calcium levels; and increased the expression and activity of antioxidant enzyme genes and the expression level of Bcl-2. Together, our data demonstrate that SOD3 ameliorates H2O2-induced oxidative damage in neuroblastoma SH-SY5Y cells by inhibiting the mitochondrial pathway and provide new insights into the functional actions of SOD3 on oxidative stress-induced cell damage.

Please choose payment method:






(PDF emailed within 0-6 h: $19.90)

Accession: 058799044

Download citation: RISBibTeXText

PMID: 27084770

DOI: 10.1007/s11064-016-1897-x


Related references

SOD3 Ameliorates Aβ 25-35 -Induced Oxidative Damage in SH-SY5Y Cells by Inhibiting the Mitochondrial Pathway. Cellular and Molecular Neurobiology 37(3): 513-525, 2016

Methylphenidate ameliorates hypoxia-induced mitochondrial damage in human neuroblastoma SH-SY5Y cells through inhibition of oxidative stress. Life Sciences 197: 40-45, 2018

Methylphenidate ameliorates hypoxia-induced mitochondrial damage in human neuroblastoma SH-SY5Y cells through inhibition of oxidative stress. Life Sciences 197: 40-45, 2018

Curcumin Ameliorates Furazolidone-Induced DNA Damage and Apoptosis in Human Hepatocyte L02 Cells by Inhibiting ROS Production and Mitochondrial Pathway. Molecules 21(8), 2017

D-saccharic acid-1,4-lactone ameliorates alloxan-induced diabetes mellitus and oxidative stress in rats through inhibiting pancreatic β-cells from apoptosis via mitochondrial dependent pathway. Toxicology and Applied Pharmacology 257(2): 272-283, 2012

Mitochondrial ferritin protects SH-SY5Y cells against H 2 O 2 -induced oxidative stress and modulates α-synuclein expression. Experimental Neurology 291: 51-61, 2017

Lycium Barbarum Polysaccharides Alleviates Oxidative Damage Induced by H2O2 Through Down-Regulating MicroRNA-194 in PC-12 and SH-SY5Y Cells. Cellular Physiology and Biochemistry 50(2): 460-472, 2018

Protective Effect of Edaravone Against Aβ25-35-Induced Mitochondrial Oxidative Damage in SH-SY5Y Cells. Cellular and Molecular Biology 63(5): 36-42, 2018

Orexin-A protects SH-SY5Y cells against H 2 O 2 -induced oxidative damage via the PI3K/MEK 1/2 /ERK 1/2 signaling pathway. International Journal of Immunopathology and Pharmacology 32: 2058738418785739, 2018

Edaravone reduces Aβ-induced oxidative damage in SH-SY5Y cells by activating the Nrf2/ARE signaling pathway. Life Sciences 2019, 2019

Crocin protects retinal ganglion cells against H2O2-induced damage through the mitochondrial pathway and activation of NF-κB. International Journal of Molecular Medicine 37(1): 225-232, 2016

Neuroprotective effects of bikaverin on H2O2-induced oxidative stress mediated neuronal damage in SH-SY5Y cell line. Cellular and Molecular Neurobiology 34(7): 973-985, 2015

Olfactory Ensheathing Cell-Conditioned Medium Reverts Aβ 25-35 -Induced Oxidative Damage in SH-SY5Y Cells by Modulating the Mitochondria-Mediated Apoptotic Pathway. Cellular and Molecular Neurobiology 37(6): 1043-1054, 2016

Buyang Huanwu Decoction attenuates H2O2-induced apoptosis by inhibiting reactive oxygen species-mediated mitochondrial dysfunction pathway in human umbilical vein endothelial cells. Bmc Complementary and Alternative Medicine 16: 154, 2017

p62 protects SH-SY5Y neuroblastoma cells against H2O2-induced injury through the PDK1/Akt pathway. Neuroscience Letters 450(1): 45-50, 2008