+ Site Statistics
+ Search Articles
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ PDF Full Text
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Translate
+ Recently Requested

Selective One-Dimensional Total Correlation Spectroscopy Nuclear Magnetic Resonance Experiments for a Rapid Identification of Minor Components in the Lipid Fraction of Milk and Dairy Products: Toward Spin Chromatography?

Selective One-Dimensional Total Correlation Spectroscopy Nuclear Magnetic Resonance Experiments for a Rapid Identification of Minor Components in the Lipid Fraction of Milk and Dairy Products: Toward Spin Chromatography?

Journal of Agricultural and Food Chemistry 63(22): 5381-5387

We report a rapid, direct, and unequivocal spin-chromatographic separation and identification of minor components in the lipid fraction of milk and common dairy products with the use of selective one-dimensional (1D) total correlation spectroscopy (TOCSY) nuclear magnetic resonance (NMR) experiments. The method allows for the complete backbone spin-coupling network to be elucidated even in strongly overlapped regions and in the presence of major components from 4 × 10(2) to 3 × 10(3) stronger NMR signal intensities. The proposed spin-chromatography method does not require any derivatization steps for the lipid fraction, is selective with excellent resolution, is sensitive with quantitation capability, and compares favorably to two-dimensional (2D) TOCSY and gas chromatography-mass spectrometry (GC-MS) methods of analysis. The results of the present study demonstrated that the 1D TOCSY NMR spin-chromatography method can become a procedure of primary interest in food analysis and generally in complex mixture analysis.

(PDF emailed within 0-6 h: $19.90)

Accession: 058820964

Download citation: RISBibTeXText

PMID: 25986319

DOI: 10.1021/acs.jafc.5b01335

Related references

Combination of matrix solid-phase dispersion extraction and direct on-line liquid chromatography-nuclear magnetic resonance spectroscopy-tandem mass spectrometry as a new efficient approach for the rapid screening of natural products: Application to the total asterosaponin fraction of the starfish Asterias rubens. Journal of Chromatography A 917(1-2): 75-86, 2001

Systematic application of two-dimensional 1H nuclear-magnetic-resonance techniques for studies of proteins. 1. Combined use of spin-echo-correlated spectroscopy and J-resolved spectroscopy for the identification of complete spin systems of non-labile protons in amino-acid residues. European Journal of Biochemistry 114(2): 365-374, 1981

Application of liquid chromatography-two-dimensional nuclear magnetic resonance spectroscopy using pre-concentration column trapping and liquid chromatography-mass spectrometry for the identification of degradation products in stressed commercial amlodipine maleate tablets. Journal of Chromatography. A 1181(1-2): 67-76, 2008

A rapid and efficient approach to metabolite identification using nuclear magnetic resonance spectroscopy, liquid chromatography/mass spectrometry and liquid chromatography/nuclear magnetic resonance spectroscopy/sequential mass spectrometry. Rapid Communications in Mass Spectrometry 12(24): 2023-2030, 1998

High resolution ¹⁹F{¹H} nuclear magnetic resonance spectroscopy and liquid chromatography-solid phase extraction-offline ¹H nuclear magnetic resonance spectroscopy for conclusive detection and identification of cyanide in water samples. Journal of Chromatography. A 1284: 88-99, 2013

Application of liquid chromatography-nuclear magnetic resonance spectroscopy to the identification of natural products. Journal of Chromatography B 748(1): 259-267, 1 October, 2000

Identification and quantification of metabolites of 2,3,5,6-tetrafluoro-4-trifluoromethylaniline in rat urine using 19F nuclear magnetic resonance spectroscopy, high-performance liquid chromatography-nuclear magnetic resonance spectroscopy and high-performance liquid chromatography-mass spectrometry. Journal of Chromatography B 748(1): 311-319, 1 October, 2000

Magnetic resonance spectroscopy of wheat proteins: a magic-angle-spinning 13C nuclear magnetic resonance and an electron spin resonance spin label study. Journal of cereal science 3(4): 319-327, 1985

Coupling liquid chromatography to mass spectrometry, infrared spectroscopy and nuclear magnetic resonance for the identification of reaction products from alpha-pinene ozonloysis. Abstracts of Papers American Chemical Society 221(1-2): ENVR 129, 2001

Identification and fractionation of human milk oligosaccharides by proton-nuclear magnetic resonance spectroscopy and reverse-phase high-performance liquid chromatography. Analytical Biochemistry 133(1): 1-8, 1983

High performance liquid chromatography coupled to nuclear magnetic resonance spectroscopy and mass spectrometry applied to plant products: identification of ecdysteroids from Silene otites. Chromatographia 49(7/8): 374-378, 1999

Direct nuclear magnetic resonance identification and quantification of geometric isomers of conjugated linoleic acid in milk lipid fraction without derivatization steps: overcoming sensitivity and resolution barriers. Analytica Chimica Acta 821: 62-71, 2015

Minor groove binding of ruphen 3 to dcgcgatcgcg 2 evidenced by two dimensional nuclear magnetic resonance spectroscopy. Journal of the American Chemical Society 114(12): 4933-4934, 1992