EurekaMag
+ Most Popular
Cunninghamia lanceolata plantations in China
Mammalian lairs in paleo ecological studies and palynology
Studies on technological possibilities in utilization of anhydrous milk fat for production of recombined butter-like products
Should right-sided fibroelastomas be operated upon?
Large esophageal lipoma
Apoptosis in the mammalian thymus during normal histogenesis and under various in vitro and in vivo experimental conditions
Poissons characoides nouveaux ou non signales de l'Ilha do Bananal, Bresil
Desensitizing efficacy of Colgate Sensitive Maximum Strength and Fresh Mint Sensodyne dentifrices
Administration of fluid by subcutaneous infusion: revival of a forgotten method
Tundra mosquito control - an impossible dream?
Schizophrenia for primary care providers: how to contribute to the care of a vulnerable patient population
Geochemical pattern analysis; method of describing the Southeastern limestone regional aquifer system
Incidence of low birth weights in a hospital of Mexico City
Tabanidae
Graded management intensity of grassland systems for enhancing floristic diversity
Microbiology and biochemistry of cheese and fermented milk
The ember tetra: a new pygmy characid tetra from the Rio das Mortes, Brazil, Hyphessobrycon amandae sp. n. (Pisces, Characoidei)
Risk factors of contrast-induced nephropathy in patients after coronary artery intervention
Renovation of onsite domestic wastewater in a poorly drained soil
Observations of the propagation velocity and formation mechanism of burst fractures caused by gunshot
Systolic blood pressure in a population of infants in the first year of life: the Brompton study
Haematological studies in rats fed with metanil yellow
Studies on pasteurellosis. I. A new species of Pasteurella encountered in chronic fowl cholera
Dormancy breaking and germination of Acacia salicina Lindl. seeds
therapy of lupus nephritis. a two-year prospective study

Small RNA and degradome deep sequencing reveals drought-and tissue-specific micrornas and their important roles in drought-sensitive and drought-tolerant tomato genotypes


Small RNA and degradome deep sequencing reveals drought-and tissue-specific micrornas and their important roles in drought-sensitive and drought-tolerant tomato genotypes



Plant Biotechnology Journal 14(8): 1727-1746



ISSN/ISBN: 1467-7644

PMID: 26857916

DOI: 10.1111/pbi.12533

Drought stress has adverse impacts on plant production and productivity. MicroRNAs (miRNAs) are one class of noncoding RNAs regulating gene expression post-transcriptionally. In this study, we employed small RNA and degradome sequencing to systematically investigate the tissue-specific miRNAs responsible to drought stress, which are understudied in tomato. For this purpose, root and upground tissues of two different drought-responsive tomato genotypes (Lycopersicon esculentum as sensitive and L. esculentum var. cerasiforme as tolerant) were subjected to stress with 5% polyethylene glycol for 7 days. A total of 699 conserved miRNAs belonging to 578 families were determined and 688 miRNAs were significantly differentially expressed between different treatments, tissues and genotypes. Using degradome sequencing, 44 target genes were identified associated with 36 miRNA families. Drought-related miRNAs and their targets were enriched functionally by Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analyses. Totally, 53 miRNAs targeted 23 key drought stress- and tissue development-related genes, including DRP (dehydration-responsive protein), GTs (glycosyltransferases), ERF (ethylene responsive factor), PSII (photosystem II) protein, HD-ZIP (homeodomain-leucine zipper), MYB and NAC-domain transcription factors. miR160, miR165, miR166, miR171, miR398, miR408, miR827, miR9472, miR9476 and miR9552 were the key miRNAs functioning in regulation of these genes and involving in tomato response to drought stress. Additionally, plant hormone signal transduction pathway genes were differentially regulated by miR169, miR172, miR393, miR5641, miR5658 and miR7997 in both tissues of both sensitive and tolerant genotypes. These results provide new insight into the regulatory role of miRNAs in drought response with plant hormone signal transduction and drought-tolerant tomato breeding.

Please choose payment method:






(PDF emailed within 0-6 h: $19.90)

Accession: 058867755

Download citation: RISBibTeXText

Related references

Small RNA and degradome profiling reveals important roles for micro RNAs and their targets in tea plant response to drought stress. Physiologia Plantarum 158(4): 435-451, 2016

Deep sequencing reveals important roles of micro RNAs in response to drought and salinity stress in cotton. Journal of Experimental Botany 66(3): 789-804, 2015

Eco-physiological response to water stress of drought-tolerant and drought-sensitive tomato genotypes. Plant Biosystems 150(4): 682-691, 2016

Drought-tolerant and drought-sensitive genotypes of maize (Zea mays L.) differ in contents of endogenous brassinosteroids and their drought-induced changes. Plos one 13(5): E0197870, 2018

Differentially expressed genes between drought-tolerant and drought-sensitive barley genotypes in response to drought stress during the reproductive stage. Journal of Experimental Botany 60(12): 3531-3544, 2009

High throughput deep degradome sequencing reveals micro RNAs and their targets in response to drought stress in mulberry (Morus alba). Plos one 12(2): E0172883, 2017

Drought tolerance mechanisms for yield responses to pre-flowering drought stress of peanut genotypes with different drought tolerant levels. Field Crops Research 144: 34-42, 2013

Physiological, biochemical and morphoagronomic characterization of drought-tolerant and drought-sensitive bean genotypes under water stress. Physiology and Molecular Biology of Plants: An International Journal of Functional Plant Biology 24(6): 1059-1067, 2018

Linked expression of several traits for drought tolerance and water use efficiency in f 2 crosses of nominally drought tolerant and drought intolerant tomato species. Plant Physiology 93(1 Suppl): 100, 1990

Metabolic responses to drought stress in the tissues of drought-tolerant and drought-sensitive wheat genotype seedlings. Aob Plants 10(2): Ply016, 2018

A comparative proteomic study of drought-tolerant and drought-sensitive soybean seedlings under drought stress. Crop and Pasture Science 67(5): 528-540, 2016

Comparative proteome analysis of drought-sensitive and drought-tolerant rapeseed roots and their hybrid F1 line under drought stress. Amino Acids 43(5): 2137-2152, 2012

High-throughput deep sequencing shows that micro RNAs play important roles in switchgrass responses to drought and salinity stress. Plant Biotechnology Journal 12(3): 354-366, 2014

Proteomic responses of drought-tolerant and drought-sensitive cotton varieties to drought stress. Molecular Genetics and Genomics: Mgg 291(3): 1293-1303, 2016

Drought tolerance and antioxidant activities in lavender plants colonized by native drought-tolerant or drought-sensitive Glomus Species. Microbial Ecology 54(3): 543-552, 2007