+ Site Statistics
References:
54,258,434
Abstracts:
29,560,870
PMIDs:
28,072,757
+ Search Articles
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ PDF Full Text
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Translate
+ Recently Requested

Special AT-rich sequence-binding protein-1 participates in the maintenance of breast cancer stem cells through regulation of the Notch signaling pathway and expression of Snail1 and Twist1



Special AT-rich sequence-binding protein-1 participates in the maintenance of breast cancer stem cells through regulation of the Notch signaling pathway and expression of Snail1 and Twist1



Molecular Medicine Reports 11(5): 3235-3542



The stem cell populations in cancerous tissues and cell lines vary widely and are often associated with aggressive cases of breast cancer. Despite research on the topic, the mechanism underlying the regulation of the breast cancer stem cell (BCSC) population within tumors remains to be fully elucidated. To investigate the function of special AT‑rich sequence‑binding protein‑1 (SATB1) in the maintenance of the BCSC population, SATB1 was overexpressed with lentivirus in MCF‑7 cells or knocked down with shRNA‑lentivirus in BT‑549 cells. The effects of SATB1 overexpression or knockdown on mammosphere formation, the size of the of BCSC population, cell invasion and tumorigenesis were investigated. Activation of the Notch signaling pathway and expression of Snail1 and Twist1 were also examined in the cells. Overexpression of SATB1 in MCF‑7 cells was observed to increase mammosphere formation, the size of the BCSC population, cell invasion and tumorigenesis, accompanied by an increase in the activation of Notch signaling and expression levels of Snail1 and Twist1. Conversely, knockdown of SATB1 in BT‑549 cells produced the opposite effects. The results indicated that expression of SATB1 may increase the size of the BCSC population via the activation of the Notch signaling pathway and by increasing expression levels of Snail1 and Twist1.

(PDF emailed within 0-6 h: $19.90)

Accession: 058884393

Download citation: RISBibTeXText

PMID: 25586771

DOI: 10.3892/mmr.2015.3192


Related references

Hsp27 participates in the maintenance of breast cancer stem cells through regulation of epithelial-mesenchymal transition and nuclear factor-κB. Breast Cancer Research 13(5): R101, 2012

WNT antagonist, DKK2, is a Notch signaling target in intestinal stem cells: Augmentation of a negative regulation system for canonical WNT signaling pathway by the Notch-DKK2 signaling loop in primates. International Journal of Molecular Medicine 19(1): 197-201, 2007

Regulation of angiogenesis via Notch signaling in breast cancer and cancer stem cells. Biochimica et Biophysica Acta 1836(2): 304-320, 2014

Tristetraprolin suppresses the EMT through the down-regulation of Twist1 and Snail1 in cancer cells. Oncotarget 7(8): 8931-8943, 2017

Upregulated SCUBE2 expression in breast cancer stem cells enhances triple negative breast cancer aggression through modulation of notch signaling and epithelial-to-mesenchymal transition. Experimental Cell Research 370(2): 444-453, 2018

Abnormal expression of the Notch and Wnt/β-catenin signaling pathways in stem-like ALDH hi CD44 + cells correlates highly with Ki-67 expression in breast cancer. Oncology Letters 9(4): 1600-1606, 2015

Monobenzyltin Complex C1 Induces Apoptosis in MCF-7 Breast Cancer Cells through the Intrinsic Signaling Pathway and through the Targeting of MCF-7-Derived Breast Cancer Stem Cells via the Wnt/β-Catenin Signaling Pathway. Plos One 11(8): E0160836, 2017

Decoy-DNA against special AT-rich sequence binding protein 1 inhibits the growth and invasive ability of human breast cancer. Oligonucleotides 21(2): 115-121, 2011

Overexpression and involvement of special AT-rich sequence binding protein 1 in multidrug resistance in human breast carcinoma cells. Cancer Science 101(1): 80-86, 2010

Regulation of sodium iodide symporter gene expression by Rac1/p38β mitogen-activated protein kinase signaling pathway in MCF-7 breast cancer cells. Journal of Biological Chemistry 287(5): 3292-3300, 2012

ErbB3 binding protein 1 (EBP1) participates in the regulation of intestinal inflammation via mediating Akt signaling pathway. Molecular Immunology 67(2 Pt B): 540-551, 2015

6-Shogaol Inhibits Breast Cancer Cells and Stem Cell-Like Spheroids by Modulation of Notch Signaling Pathway and Induction of Autophagic Cell Death. Plos One 10(9): E0137614, 2016

Expression of special AT-rich sequence-binding protein mRNA and its clinicopathological significance in non-small cell lung cancer. Nan Fang Yi Ke Da Xue Xue Bao 29(3): 534-537, 2010

Fascin Is Critical for the Maintenance of Breast Cancer Stem Cell Pool Predominantly via the Activation of the Notch Self-Renewal Pathway. Stem Cells 34(12): 2799-2813, 2016

Special AT-rich sequence-binding protein 2 acts as a negative regulator of stemness in colorectal cancer cells. World Journal of Gastroenterology 22(38): 8528-8539, 2016