+ Site Statistics
References:
54,258,434
Abstracts:
29,560,870
PMIDs:
28,072,757
+ Search Articles
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ PDF Full Text
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Translate
+ Recently Requested

Stimulated Raman photoacoustic spectroscopy for chemical-contrast imaging of a sample deeply buried in scattering media



Stimulated Raman photoacoustic spectroscopy for chemical-contrast imaging of a sample deeply buried in scattering media



Analyst 141(20): 5747-5752



The development of a stimulated Raman scattering photoacoustic (SRS-PA) spectrometer is presented. In the apparatus, a molecular vibrational mode is excited by the SRS process. The vibrational excitation energy is converted to heat by vibrational relaxation. The volumes around the excited molecules including the surrounding solvent molecules expand by heating, resulting in the generation of an ultrasonic wave. The ultrasonic wave can be used as a molecular-selective signal. Because the ultrasonic wave is scarcely scattered by media, SRS-PA is expected to be applied for obtaining molecular-selective signals from deeply buried samples. In the present study, a SRS-PA spectrometer was developed and applied to obtain molecular-selective signals from test samples. The SRS-PA signals from water and lipid, which are important components in biological systems, were first obtained. The SRS-PA signal from a polystyrene film buried in a highly light-scattering intralipid suspension was also measured. We succeeded in obtaining the signal from the film when it was buried with a depth of up to 1.8 mm. The results indicate that SRS-PA can be effectively applied for the chemical-contrast imaging of deeply buried samples.

(PDF emailed within 0-6 h: $19.90)

Accession: 058905709

Download citation: RISBibTeXText

PMID: 27508283

DOI: 10.1039/c6an01211f


Related references

Raman Spectroscopy: Stimulated Raman Scattering Microscopyfor Label-Free Chemical Imaging. Optics and Photonics News 20(12): 30-0, 2009

A pulsed photoacoustic system for the spectroscopy and monitoring of hydrocarbon liquids using stimulated Raman scattering in a silica fibre as a near-infrared source. Measurement Science and Technology 10(2): 93-99, 1999

High contrast three-dimensional photoacoustic imaging through scattering media by localized optical fluence enhancement. Optics Express 21(22): 26671-6, 2014

Time-gated imaging through scattering media using stimulated Raman amplification. Optics Letters 16(23): 1868-1870, 1991

Hyperspectral imaging with in-line interferometric femtosecond stimulated Raman scattering spectroscopy. Journal of Chemical Physics 140(8): 084201, 2014

Quantitative chemical imaging with stimulated Raman scattering microscopy. Current Opinion in Chemical Biology 39: 24-31, 2017

Three-dimensional chemical imaging of skin using stimulated Raman scattering microscopy. Journal of Biomedical Optics 19(11): 111604, 2015

Quantitative chemical imaging with multiplex stimulated Raman scattering microscopy. Journal of the American Chemical Society 134(8): 3623-3626, 2012

Monitoring stimulated Raman scattering with photoacoustic detection. Optics Letters 36(7): 1233-1235, 2011

Multimodality Raman and photoacoustic imaging of surface-enhanced-Raman-scattering-targeted tumor cells. Journal of Biomedical Optics 21(2): 20503, 2016

Label-free imaging of lipid dynamics using Coherent Anti-stokes Raman Scattering (CARS) and Stimulated Raman Scattering (SRS) microscopy. Current Opinion in Genetics and Development 21(5): 585-590, 2012

Volumetric chemical imaging by clearing-enhanced stimulated Raman scattering microscopy. Proceedings of the National Academy of Sciences of the United States of America 2019, 2019

Label-Free Chemical Imaging of Latent Fingerprints with Stimulated Raman Scattering Microscopy. Analytical Chemistry 89(8): 4468-4473, 2017

Prospects of deep Raman spectroscopy for noninvasive detection of conjugated surface enhanced resonance Raman scattering nanoparticles buried within 25 mm of mammalian tissue. Analytical Chemistry 82(10): 3969-3973, 2010

Live-Cell Bioorthogonal Chemical Imaging: Stimulated Raman Scattering Microscopy of Vibrational Probes. Accounts of Chemical Research 49(8): 1494-1502, 2018