+ Site Statistics
+ Search Articles
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ PDF Full Text
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Translate
+ Recently Requested

Stimulated Raman scattering microscopy: an emerging tool for drug discovery

Stimulated Raman scattering microscopy: an emerging tool for drug discovery

Chemical Society Reviews 45(8): 2075-2089

Optical microscopy techniques have emerged as a cornerstone of biomedical research, capable of probing the cellular functions of a vast range of substrates, whilst being minimally invasive to the cells or tissues of interest. Incorporating biological imaging into the early stages of the drug discovery process can provide invaluable information about drug activity within complex disease models. Spontaneous Raman spectroscopy has been widely used as a platform for the study of cells and their components based on chemical composition; but slow acquisition rates, poor resolution and a lack of sensitivity have hampered further development. A new generation of stimulated Raman techniques is emerging which allows the imaging of cells, tissues and organisms at faster acquisition speeds, and with greater resolution and sensitivity than previously possible. This review focuses on the development of stimulated Raman scattering (SRS), and covers the use of bioorthogonal tags to enhance sample detection, and recent applications of both spontaneous Raman and SRS as novel imaging platforms to facilitate the drug discovery process.

(PDF emailed within 0-6 h: $19.90)

Accession: 058905715

Download citation: RISBibTeXText

PMID: 26839248

DOI: 10.1039/c5cs00693g

Related references

Imaging drug delivery to skin with stimulated Raman scattering microscopy. Molecular Pharmaceutics 8(3): 969-975, 2011

Imaging drug uptake by bioorthogonal stimulated Raman scattering microscopy. Chemical Science 8(8): 5606-5615, 2018

Label-free imaging of lipid dynamics using Coherent Anti-stokes Raman Scattering (CARS) and Stimulated Raman Scattering (SRS) microscopy. Current Opinion in Genetics & Development 21(5): 585-590, 2012

Molecular orientational order probed by coherent anti-Stokes Raman scattering (CARS) and stimulated Raman scattering (SRS) microscopy: a spectral comparative study. Journal of Physical Chemistry. B 119(7): 3242-3249, 2016

Stimulated-emission-depletion microscopy with a multicolor stimulated-Raman-scattering light source. Optics Letters 33(21): 2491-2493, 2008

Lock-in-detection-free line-scan stimulated Raman scattering microscopy for near video-rate Raman imaging. Optics Letters 41(17): 3960-3963, 2016

Spontaneous generation of phase waves and solitons in stimulated Raman scattering: Quantum-mechanical models of stimulated Raman scattering. Physical Review. A, Atomic, Molecular, and Optical Physics 42(5): 2870-2889, 1990

Multicolor stimulated Raman scattering (SRS) microscopy. Molecular Physics 110(15-16): 1927-1932, 2013

Rationalizing inter- and intracrystal heterogeneities in dealuminated acid mordenite zeolites by stimulated Raman scattering microscopy correlated with super-resolution fluorescence microscopy. Acs Nano 8(12): 12650-9, 2014

Electronic Pre-resonance Stimulated Raman Scattering Microscopy. Journal of Physical Chemistry Letters: -, 2018

In vivo Imaging with Stimulated Raman Scattering Microscopy. Optics and Photonics News 22(12): 27-0, 2011

STED microscopy with a MHz pulsed stimulated-Raman-scattering source. Optics Express 17(18): 15679-15684, 2009

Continuous-Wave Stimulated Raman Scattering (cwSRS) Microscopy. Applied Physics. B, Lasers and Optics 112(1): 99-103, 2013

Quantitative chemical imaging with stimulated Raman scattering microscopy. Current Opinion in Chemical Biology 39: 24-31, 2017

Amplitude and polarization modulated hyperspectral Stimulated Raman Scattering Microscopy. Optics Express 23(22): 28119-28131, 2015