+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Tagging and mapping of SSR marker for rust resistance gene in lentil (Lens culinaris Medikus subsp. culinaris)



Tagging and mapping of SSR marker for rust resistance gene in lentil (Lens culinaris Medikus subsp. culinaris)



Indian Journal of Experimental Biology 54(6): 394-399



Lentil, as an economical source of protein, minerals and vitamins, plays important role in nutritional security of the common man. Grown mainly in West Asia, North Africa (WANA) region and South Asia, it suffers from several biotic stresses such as wilt, rust, blight and broomrape. Lentil rust caused by autoecious fungus Uromyces viciae fabae (Pers.) Schroet is a serious lentil disease in Algeria, Bangladesh, Ethiopia, India, Italy, Morocco, Pakistan and Nepal. The disease symptoms are observed during flowering and early podding stages. Rust causes severe yield losses in lentil. It can only be effectively controlled by identifying the resistant source, understanding its inheritance and breeding for host resistance. The obligate parasitic nature of pathogen makes it difficult to maintain the pathogen in culture and to apply it to screen segregating progenies under controlled growth conditions. Hence, the use of molecular markers will compliment in identification of resistant types in different breeding programs. Here, we studied the inheritance of resistance to rust in lentil using F₁, F₂ and F₂:₃ from cross PL 8 (susceptible) x L 4149 (resistant) varieties. The phenotyping of lentil population was carried out at Sirmour, India. The result of genetic analysis revealed that a single dominant gene controls rust resistance in lentil genotype L 4149. The F2 population from this cross was used to tag and map the rust resistance gene using SSR and SRAP markers. Markers such as 270 SRAP and 162 SSR were studied for polymorphism and 101 SRAP and 33 SSRs were found to be polymorphic between the parents. Two SRAP and two SSR markers differentiated the resistant and susceptible bulks. SSR marker Gllc 527 was estimated to be linked to rust resistant locus at a distance of 5.9 cM. The Gllc 527 marker can be used for marker assisted selection for rust resistance; however, additional markers closer to rust resistant locus are required. The markers linked to the rust resistance gene can serve as starting points for map-based cloning of the rust resistance gene.

Please choose payment method:






(PDF emailed within 1 workday: $29.90)

Accession: 058969311

Download citation: RISBibTeXText

PMID: 27468466


Related references

Diversification of indigenous gene- pool by using exotic germplasm in lentil (Lens culinaris Medikus subsp. culinaris). Physiology and Molecular Biology of Plants 20(1): 125-132, 2014

Inheritance and linkage relationships of days to flower and morphological loci in lentil (Lens culinaris Medikus subsp. culinaris). Journal of Heredity 90(2): 270-275, 1999

Selenium biofortification in lentil (Lens culinaris Medikus subsp. culinaris): Farmers' field survey and genotypeenvironment effect. Food Research International 54(2): 1596-1604, 2013

Improving breeding efficiency through application of appropriate experimental designs and analysis models: A case of lentil (Lens culinaris Medikus subsp. culinaris) yield trials. Field Crops Research 179: 26-34, 2015

New sources of resistance to Colletotrichum truncatum race Ct0 and Ct1 in Lens culinaris Medikus subsp culinaris obtained by single plant selection in germplasm access. 2013

New sources of resistance to Colletotrichum truncatum race Ct0 and Ct1 in Lens culinaris Medikus subsp culinaris obtained by single plant selection in germplasm accessions. Genetic Resources and Crop Evolution 60(1): 193-201, 2013

New sources of resistance to Colletotrichum truncatum race Ct and Ct1 in Lens culinaris Medikus subsp culinaris obtained by single plant selection in germplasm accessions. 2012

Association mapping unveils favorable alleles for grain iron and zinc concentrations in lentil (Lens culinaris subsp. culinaris). Plos One 12(11): E0188296, 2017

Protein content in wild and cultivated taxa of lentil (Lens culinaris ssp culinaris Medikus). Indian Journal of Genetics and Plant Breeding 76(4): 631-634, 2016

Expression of the DREB1A gene in lentil (Lens culinaris Medik. subsp culinaris) transformed with the Agrobacterium system. Crop and Pasture Science 62(6): 488-495, 2011

Seasonal occurrence of the main groups of insect pests and natural enemies associated with lentil Lens culinaris Medikus in Castilla-La Mancha central Spain Variacion estacional de los principales grupos de insectos plaga y enemigos naturales asociados al cultivo de la lenteja Lens culinaris Medikus en Castilla-La Mancha. Boletin de Sanidad Vegetal Plagas. 4o Trimestre; 24: 955-974 (Adenda al No 4, 1998

Genetic analysis and molecular mapping of seedling survival drought tolerance gene in lentil (Lens culinaris Medikus). Molecular Breeding 36(5): 58, 2016

Genetics of resistance to anthracnose and identification of AFLP and RAPD markers linked to the resistance gene in PI 320937 germplasm of lentil (Lens culinaris Medikus). Tag. Theoretical and Applied Genetics. Theoretische und Angewandte Genetik 106(3): 428-434, 2003

Molecular mapping of aluminium resistance loci based on root re-growth and Al-induced fluorescent signals (callose accumulation) in lentil (Lens culinaris Medikus). Molecular Biology Reports 2018, 2018

Enhancing selenium concentration in lentil (Lens culinaris subsp culinaris) through foliar application. Journal of Agricultural Science 153(4): 656-665, 2015