EurekaMag
+ Translate
+ Most Popular
Cunninghamia lanceolata plantations in China
Mammalian lairs in paleo ecological studies and palynology
Studies on technological possibilities in utilization of anhydrous milk fat for production of recombined butter-like products
Should right-sided fibroelastomas be operated upon?
Large esophageal lipoma
Apoptosis in the mammalian thymus during normal histogenesis and under various in vitro and in vivo experimental conditions
Poissons characoides nouveaux ou non signales de l'Ilha do Bananal, Bresil
Desensitizing efficacy of Colgate Sensitive Maximum Strength and Fresh Mint Sensodyne dentifrices
Administration of fluid by subcutaneous infusion: revival of a forgotten method
Tundra mosquito control - an impossible dream?
Schizophrenia for primary care providers: how to contribute to the care of a vulnerable patient population
Geochemical pattern analysis; method of describing the Southeastern limestone regional aquifer system
Incidence of low birth weights in a hospital of Mexico City
Tabanidae
Graded management intensity of grassland systems for enhancing floristic diversity
Microbiology and biochemistry of cheese and fermented milk
The ember tetra: a new pygmy characid tetra from the Rio das Mortes, Brazil, Hyphessobrycon amandae sp. n. (Pisces, Characoidei)
Risk factors of contrast-induced nephropathy in patients after coronary artery intervention
Renovation of onsite domestic wastewater in a poorly drained soil
Observations of the propagation velocity and formation mechanism of burst fractures caused by gunshot
Systolic blood pressure in a population of infants in the first year of life: the Brompton study
Haematological studies in rats fed with metanil yellow
Studies on pasteurellosis. I. A new species of Pasteurella encountered in chronic fowl cholera
Dormancy breaking and germination of Acacia salicina Lindl. seeds
therapy of lupus nephritis. a two-year prospective study

The Epstein-Barr virus BRRF2 gene product is involved in viral progeny production


The Epstein-Barr virus BRRF2 gene product is involved in viral progeny production



Virology 484: 33-40



ISSN/ISBN: 0042-6822

PMID: 26057150

DOI: 10.1016/j.virol.2015.05.010

The Epstein-Barr virus (EBV) predominantly establishes a latent infection in B lymphocytes, and occasionally switches from the latent state to the lytic cycle. In this report, we identified and examined the role of a lytic gene, BRRF2. We first prepared an antibody against BRRF2 and identified the gene product as a viral lytic protein expressed in B95-8 cells with late kinetics. Immunofluorescence revealed that BRRF2 localized in the cytoplasm of cells during the lytic phase. We also found that BRRF2 protein was phosphorylated in lytic cells, but the only viral protein kinase, BGLF4, was not involved in the phosphorylation. Knockout EBV and a repaired strain were then prepared, and we found that BRRF2 disruption did not affect viral gene expression and DNA replication, but decreased virus production. These results demonstrated that BRRF2 is involved in production of infectious progeny, although it is not essential for lytic replication.

Please choose payment method:






(PDF emailed within 0-6 h: $19.90)

Accession: 059005992

Download citation: RISBibTeXText

Related references

Epstein-Barr Virus BKRF4 Gene Product Is Required for Efficient Progeny Production. Journal of Virology 91(23), 2017

Epstein-Barr virus BCRF1 gene product (viral interleukin 10) inhibits superoxide anion production by human monocytes. Lymphokine and Cytokine Research 11(5): 209-214, 1992

The Epstein-Barr virus immediate-early gene product, BRLF1, interacts with the retinoblastoma protein during the viral lytic cycle. Journal of Virology 72(10): 8043-8051, 1998

Relationship between the amounts of epstein barr virus dna and epstein barr virus nuclear antigen per cell clonability and tumorigenicity in 2 epstein barr virus negative lymphoma lines and their epstein barr virus converted sublines. International Journal of Cancer 31(2): 163-170, 1983

A unique mechanism unrelated to epstein barr virus ebv w z gene rearrangement may control viral production. Proceedings of the American Association for Cancer Research Annual Meeting 32: 310, 1991

Aberrant Epstein-Barr virus persistence in HIV carriers is characterized by anti-Epstein-Barr virus IgA and high cellular viral loads with restricted transcription. Aids 21(16): 2141-2149, 2007

Viral DNA contamination is responsible for Epstein-Barr virus detection in cytotoxic T lymphocytes stimulated in vitro with Epstein-Barr virus B-lymphoblastoid cell line. Cancer Immunology Immunotherapy: Cii 59(12): 1867-1875, 2010

Documentation of Epstein-Barr virus infection in immunodeficient patients with life-threatening lymphoproliferative diseases by Epstein-Barr virus complementary RNA/DNA and viral DNA/DNA hybridization. Cancer Research 41(11 Pt 1): 4237-4242, 1981

Serum Epstein-Barr virus (EBV) viral load can be a complementary sensitive test in primary Epstein-Barr virus infection. Journal of Clinical Virology: the Official Publication of the Pan American Society for Clinical Virology 50(2): 184-185, 2011

Transfection of epstein barr virus dna fragments coding for epstein barr virus nuclear antigen 1 and epstein barr virus nuclear antigen 2 into human lymphocytes gene expression without cell immortalization. Journal of Cellular Biochemistry Suppl. (10 Part A): 213, 1986

Establishment of epstein barr virus nuclear antigen expressing cell lines by infection of epstein barr virus genome negative human lymphoma cells with different epstein barr virus strains. International Journal Of Cancer: 161-166, 1976

An Epstein-Barr virus immediate-early gene product trans-activates gene expression from the human immunodeficiency virus long terminal repeat. Proceedings of the National Academy of Sciences of the United States of America 85(5): 1652-1656, 1988

Epstein-Barr viral nuclear antigen 1 antisense oligodeoxynucleotide inhibits proliferation of Epstein-Barr virus-immortalized B cells. Blood 84(2): 582-587, 1994

Molecular size variation of epstein barr virus induced nuclear antigen is determined by the epstein barr viral genome. Acta Microbiologica Hungarica 31(3): 259-260, 1984

Restricted expression of latency-associated viral genes and production of viral IL-10 in Epstein-Barr virus-related NK/T-cell lymphomas. Journal of Dermatological Science 23(3): 198, 2000