EurekaMag.com logo
+ Site Statistics
References:
52,654,530
Abstracts:
29,560,856
PMIDs:
28,072,755
+ Search Articles
+ Subscribe to Site Feeds
EurekaMag Most Shared ContentMost Shared
EurekaMag PDF Full Text ContentPDF Full Text
+ PDF Full Text
Request PDF Full TextRequest PDF Full Text
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter
Follow on LinkedInFollow on LinkedIn

+ Translate

The Ska complex promotes Aurora B activity to ensure chromosome biorientation



The Ska complex promotes Aurora B activity to ensure chromosome biorientation



Journal of Cell Biology 215(1): 77-93



Chromosome biorientation and accurate segregation rely on the plasticity of kinetochore-microtubule (KT-MT) attachments. Aurora B facilitates KT-MT dynamics by phosphorylating kinetochore proteins that are critical for KT-MT interactions. Among the substrates whose microtubule and kinetochore binding is curtailed by Aurora B is the spindle and kinetochore-associated (Ska) complex, a key factor for KT-MT stability. Here, we show that Ska is not only a substrate of Aurora B, but is also required for Aurora B activity. Ska-deficient cells fail to biorient and display chromosome segregation errors underlying suppressed KT-MT turnover. These defects coincide with KNL1-Mis12-Ndc80 network hypophosphorylation, reduced mitotic centromere-associated kinesin localization, and Aurora B T-loop phosphorylation at kinetochores. We further show that Ska requires its microtubule-binding capability to promote Aurora B activity in cells and stimulates Aurora B catalytic activity in vitro. Finally, we show that protein phosphatase 1 counteracts Aurora B activity to enable Ska kinetochore accumulation once biorientation is achieved. We propose that Ska promotes Aurora B activity to limit its own microtubule and kinetochore association and to ensure that KT-MT dynamics and stability fall within an optimal balance for biorientation.

(PDF emailed within 0-6 h: $19.90)

Accession: 059032063

Download citation: RISBibTeXText

PMID: 27697923

DOI: 10.1083/jcb.201603019



Related references

Dispensability of the SAC Depends on the Time Window Required by Aurora B to Ensure Chromosome Biorientation. Plos One 10(12): E0144972, 2016

An Mtw1 complex promotes kinetochore biorientation that is monitored by the Ipl1/Aurora protein kinase. Developmental Cell 5(5): 735-745, 2003

Feedback control in sensing chromosome biorientation by the Aurora B kinase. Current Biology 21(13): 1158-1165, 2011

Sgo1 regulates both condensin and Ipl1/Aurora B to promote chromosome biorientation. Plos Genetics 10(6): E1004411, 2015

The S. pombe Cdc14-like phosphatase Clp1p regulates chromosome biorientation and interacts with Aurora kinase. Developmental Cell 7(5): 755-762, 2004

Bub1 kinase targets Sgo1 to ensure efficient chromosome biorientation in budding yeast mitosis. Plos Genetics 3(11): E213, 2007

Mps1 promotes chromosome meiotic chromosome biorientation through Dam1. Molecular Biology of the Cell 29(4): 479-489, 2017

BUB-1 promotes amphitelic chromosome biorientation via multiple activities at the kinetochore. Elife 7, 2018

Evidence that the Ipl1-Sli15 (Aurora kinase-INCENP) complex promotes chromosome bi-orientation by altering kinetochore-spindle pole connections. Cell 108(3): 7-29, 2002

Aurora-C Interactions with Survivin and INCENP Reveal Shared and Distinct Features Compared with Aurora-B Chromosome Passenger Protein Complex. Plos One 11(6): E0157305, 2017

The chromosomal passenger complex is required for meiotic acentrosomal spindle assembly and chromosome biorientation. Genetics 192(2): 417-429, 2013

Ipl1/Aurora kinase suppresses S-CDK-driven spindle formation during prophase I to ensure chromosome integrity during meiosis. Plos One 8(12): E83982, 2014

INCENP-aurora B interactions modulate kinase activity and chromosome passenger complex localization. Journal of Cell Biology 187(5): 637-653, 2010

Chromosome biorientation and APC activity remain uncoupled in oocytes with reduced volume. Journal of Cell Biology 216(12): 3949-3957, 2017

An engineered minimal chromosomal passenger complex reveals a role for INCENP/Sli15 spindle association in chromosome biorientation. Journal of Cell Biology 216(4): 911-923, 2017