+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

The distribution pattern of α2,3- and α2,6-linked sialic acids affects host cell preference in Toxoplasma gondii



The distribution pattern of α2,3- and α2,6-linked sialic acids affects host cell preference in Toxoplasma gondii



Experimental Parasitology 155: 74-81



Tachyzoites of Toxoplasma gondii, an obligate intracellular parasite, actively invade almost all types of nucleated cells. However, T. gondii tachyzoites preferentially infect particular types of animal tissue cells. The mechanism underlying the host cell preference of T. gondii is not yet known. In this study, we found that enzymatic removal of α2,3- but not α2,6-linked sialic acids on the surface of Vero cells decreased T. gondii tachyzoite adhesion or invasion to the treated cells. Although Chinese hamster ovary (CHO) cells express only α2,3-linked sialic acid, a genetically modified CHO cell line constructed by transfection with the α2,6-sialiltransferase gene contains subpopulations with a variety of expression patterns of α2,3- and α2,6-linked sialic acids. When T. gondii tachyzoites were added to the modified CHO cells, the tachyzoites preferentially attached to cells belonging to a subpopulation of cells that highly expressed α2,3-linked sialic acids. Additionally, multiple regression analysis performed to analyse the relationship between the amount of α2,3-linked/α2,6-linked sialic acids and parasite-expressed fluorescence intensity suggested that more tachyzoites adhered to individual α2,3-linked sialic acid rich-cells than to α2,3-linked sialic acid-poor/null cells. The results of confocal laser microscopy confirmed this finding. These results indicate that the host cell preference of T. gondii was, at least partially, affected by the distribution pattern of α2,3-, but almost never α2,6-linked sialic acids on host cells.

Please choose payment method:






(PDF emailed within 0-6 h: $19.90)

Accession: 059054300

Download citation: RISBibTeXText

PMID: 26003519

DOI: 10.1016/j.exppara.2015.05.005


Related references

Sialic acid residues of host cell are involved in the process of Toxoplasma gondii penetration. Memorias do Instituto Oswaldo Cruz 92(Suppl. 1): 79, 1997

Host cell surface sialic acid residues are involved on the process of penetration of Toxoplasma gondii into mammalian cells. Fems Microbiology Letters 164(2): 323-327, 1998

Host Cell Preference of Toxoplasma gondii Cysts in Murine Brain: A Confocal Study. Journal of Neuroparasitology 1:, 2010

Rhomboid 4 (ROM4) affects the processing of surface adhesins and facilitates host cell invasion by Toxoplasma gondii. Plos Pathogens 6(4): E1000858, 2010

Kinetics and pattern of organelle exocytosis during Toxoplasma gondii/host-cell interaction. Parasitology Research 79(5): 402-408, 1993

Toxoplasma gondii glycosylphosphatidylinositols are not involved in T. gondii-induced host cell survival. Apoptosis 12(4): 781-790, 2007

Toxoplasma gondii acyl-lipid metabolism: de novo synthesis from apicoplast-generated fatty acids versus scavenging of host cell precursors. Biochemical Journal 394(Pt 1): 197-205, 2006

Lysosomes of Toxoplasma gondii and their possible relation to the host-cell penetration of toxoplasma parasites. Journal of Bacteriology 96(4): 916-919, 1968

H7N9 influenza viruses interact preferentially with α2,3-linked sialic acids and bind weakly to α2,6-linked sialic acids. Journal of General Virology 94(Pt 11): 2417-2423, 2013

Coordinate control of host centrosome position, organelle distribution, and migratory response by Toxoplasma gondii via host mTORC2. Journal of Biological Chemistry 285(20): 15611-8, 2010

Entry of influenza A Virus with a α2,6-linked sialic acid binding preference requires host fibronectin. Journal of Virology 86(19): 10704-10713, 2012

An in silico pipeline to filter the Toxoplasma gondii proteome for proteins that could traffic to the host cell nucleus and influence host cell epigenetic regulation. Memorias do Instituto Oswaldo Cruz 113(6): E170471, 2018

Role of Toxoplasma gondii HSP70 and Toxoplasma gondii HSP30/bag1 in antibody formation and prophylactic immunity in mice experimentally infected with Toxoplasma gondii. Microbiology and Immunology 43(5): 471-479, 1999

Attachment of Toxoplasma gondii to host cells is host cell cycle dependent. Infection and Immunity 64(10): 4099-4104, 1996

Host cell invasion by Toxoplasma gondii is temporally regulated by the host microtubule cytoskeleton. Eukaryotic Cell 9(11): 1680-1689, 2010