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The effect of freeze-thawing on magnetic resonance imaging T2* of freshly harvested bovine patellar tendon



The effect of freeze-thawing on magnetic resonance imaging T2* of freshly harvested bovine patellar tendon



Quantitative Imaging in Medicine and Surgery 5(3): 368-373



Analysis of fresh specimens in research studies is ideal; however, it is often necessary to freeze samples for evaluation at a later time. Limited evaluation of the effect of freeze-thawing of tendon tissue samples on inherent magnetic resonance imaging (MRI) parameters, such as ultrashort echo time (UTE) T2* values, have been performed to date. This study performed UTE MRI on 14 bovine patellar tendons at harvest and after four consecutive freeze-thaw cycles. Results demonstrated a small but significant reduction (12%) in tendon T2* values after the first freeze thaw cycle, but not after successive cycles. Tendons from juvenile animals with open physis had a significant reduction of T2* following a single freeze thaw cycle, P<0.0001. The results of this study emphasize the importance of using uniform tendon storage protocols when using UTE MRI in preclinical models.

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Accession: 059057807

Download citation: RISBibTeXText

PMID: 26029639

DOI: 10.3978/j.issn.2223-4292.2015.03.14


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