+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

The effectively specific recognition of bovine serum albumin imprinted silica nanoparticles by utilizing a macromolecularly functional monomer to stabilize and imprint template



The effectively specific recognition of bovine serum albumin imprinted silica nanoparticles by utilizing a macromolecularly functional monomer to stabilize and imprint template



Analytica Chimica Acta 884: 97-105



Structural stability of the template is one of the most important considerations during the preparation of protein imprinting technology. To address this limitation, we propose a novel and versatile strategy of utilizing macromolecularly functional monomers to imprint biomacromolecules. Results from circular dichroism and synchronous fluorescence experiments reflect the macromolecularly functional monomers tendency to interact with the protein surface instead of permeating it and destroying the hydrogen bonds that maintain the protein's structural stability, therefore stabilizing the template protein structure during the preparation of imprinted polymers. The imprinted polymers composed of macromolecularly functional monomers or their equivalent micromolecularly functional monomers over silica nanoparticles were characterized and carried out in batch rebinding test and competitive adsorption experiments. In batch rebinding test, the imprinted particles prepared with macromolecularly functional monomers exhibited an imprinting factor of 5.8 compared to those prepared by micromolecularly functional monomers with the imprinting factor of 3.4. The selective and competitive adsorption experiments also demonstrated the imprinted particles made by macromolecularly functional monomers possessed much better selectivity and specific recognition ability for template protein. Therefore, using macromolecularly functional monomers to imprint may overcome the mutability of biomacromolecule typically observed during the preparation of imprinted polymers, and thus promote the further development of imprinting technology.

Please choose payment method:






(PDF emailed within 0-6 h: $19.90)

Accession: 059061243

Download citation: RISBibTeXText

PMID: 26073815

DOI: 10.1016/j.aca.2015.05.015


Related references

m-Aminophenylboronic Acid as a Functional Monomer for Fabricating Molecularly Imprinted Polymer for the Recognition of Bovine Serum Albumin. Reactive & Functional Polymers 68(9): 1291-1296, 2008

Fabrication of a surface imprinted hydrogel shell over silica microspheres using bovine serum albumin as a model protein template. Biosensors & Bioelectronics 25(3): 615-622, 2010

One-step preparation of magnetic imprinted nanoparticles adopting dopamine-cupric ion as a co-monomer for the specific recognition of bovine hemoglobin. Journal of Separation Science 38(20): 3568-3574, 2016

The preparation of bovine serum albumin surface-imprinted superparamagnetic polymer with the assistance of basic functional monomer and its application for protein separation. Journal of Chromatography. A 1218(22): 3489-3495, 2011

Epitope imprinted polymer nanoparticles containing fluorescent quantum dots for specific recognition of human serum albumin. Microchimica Acta 182(7-8): 1465-1472, 2015

Green synthesis of hydrophilic protein-imprinted resin with specific recognition of bovine serum albumin in aqueous matrix. Analytica Chimica Acta 1033: 213-220, 2018

A functional monomer is not enough: principal component analysis of the influence of template complexation in pre-polymerization mixtures on imprinted polymer recognition and morphology. International Journal of Molecular Sciences 15(11): 20572-20584, 2015

Functional monomer-template-QDs sandwich structure for mesoporous structured bovine hemoglobin imprinted ratiometric fluorescence sensor. Talanta 165: 482-488, 2017

Preparation and specific recognition of protein macromolecularly imprinted polyampholyte hydrogel. Talanta 192: 14-23, 2018

Molecularly imprinted electrochemical sensing interface based on in-situ-polymerization of amino-functionalized ionic liquid for specific recognition of bovine serum albumin. Biosensors & Bioelectronics 74: 792-798, 2016

Epitope imprinted polymer coating CdTe quantum dots for specific recognition and direct fluorescent quantification of the target protein bovine serum albumin. Biosensors & Bioelectronics 54: 266-272, 2014

Preparation of magnetic glycoprotein-imprinted nanoparticles with dendritic polyethyleneimine as a monomer for the specific recognition of ovalbumin from egg white. Journal of Separation Science 39(10): 1919-1925, 2016

Preparation and adsorption characteristics of surface-imprinted polymer using bovine serum albumin and lysozyme as double template proteins. Se Pu 33(5): 475-480, 2015

Preparation of magnetic molecularly imprinted polymers based on a deep eutectic solvent as the functional monomer for specific recognition of lysozyme. Mikrochimica Acta 185(2): 146-146, 2018

Bovine serum albumin recognition via thermosensitive molecular imprinted macroporous hydrogels prepared at two different temperatures. Analytica Chimica Acta 723: 45-53, 2012