+ Site Statistics
References:
54,258,434
Abstracts:
29,560,870
PMIDs:
28,072,757
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

The histone methyltransferase SDG8 mediates the epigenetic modification of light and carbon responsive genes in plants



The histone methyltransferase SDG8 mediates the epigenetic modification of light and carbon responsive genes in plants



Genome Biology 16: 79



Histone methylation modifies the epigenetic state of target genes to regulate gene expression in the context of developmental and environmental changes. Previously, we used a positive genetic screen to identify an Arabidopsis mutant, cli186, which was impaired in carbon and light signaling. Here, we report a deletion of the Arabidopsis histone methyltransferase SDG8 in this mutant (renamed sdg8-5), which provides a unique opportunity to study the global function of a specific histone methyltransferase within a multicellular organism. To assess the specific role of SDG8, we examine how the global histone methylation patterns and transcriptome were altered in the sdg8-5 deletion mutant compared to wild type, within the context of transient light and carbon treatments. Our results reveal that the sdg8 deletion is associated with a significant reduction of H3K36me3, preferentially towards the 3' end of the gene body, accompanied by a reduction in gene expression. We uncover 728 direct targets of SDG8 that have altered methylation in the sdg8-5 mutant and are also bound by SDG8. As a group, this set of SDG8 targets is enriched in specific biological processes including defense, photosynthesis, nutrient metabolism and energy metabolism. Importantly, 64% of these SDG8 targets are responsive to light and/or carbon signals. The histone methyltransferase SDG8 functions to regulate the H3K36 methylation of histones associated with gene bodies in Arabidopsis. The H3K36me3 mark in turn is associated with high-level expression of a specific set of light and/or carbon responsive genes involved in photosynthesis, metabolism and energy production.

Please choose payment method:






(PDF emailed within 0-6 h: $19.90)

Accession: 059073219

Download citation: RISBibTeXText

PMID: 25928034

DOI: 10.1186/s13059-015-0640-2


Related references

The histone methyltransferase SDG8 regulates shoot branching in Arabidopsis. Biochemical and Biophysical Research Communications 373(4): 659-664, 2008

Epigenetic modification in gliomas: role of the histone methyltransferase EZH2. Expert Opinion on Therapeutic Targets 18(10): 1197-1206, 2015

Histone lysine methyltransferase SDG8 is involved in brassinosteroid-regulated gene expression in Arabidopsis thaliana. Molecular Plant 7(8): 1303-1315, 2015

Regulation of Carotenoid Composition and Shoot Branching in Arabidopsis by a Chromatin Modifying Histone Methyltransferase, Sdg8. The Plant Cell 21(1): 39-53, 2009

Regulation of carotenoid composition and shoot branching in Arabidopsis by a chromatin modifying histone methyltransferase, SDG8. Plant Cell 21(1): 39-53, 2009

Uncovering the mechanistic basis for specific recognition of monomethylated H3K4 by the CW domain of Arabidopsis histone methyltransferase SDG8. Journal of Biological Chemistry 293(17): 6470-6481, 2018

Arabidopsis histone methyltransferase SET DOMAIN GROUP2 is required for regulation of various hormone responsive genes. Journal of Plant Biology 56(1): 39-48, 2013

The Ezh2 Histone Methyltransferase Contributes To Pancreatic Cancer Cell Proliferation Through Epigenetic Silencing Of Cdk Inhibitor Genes. Pancreas 35(4): 420-421, 2007

Epigenetic transitions in plants not associated with changes in DNA or histone modification. Biochimica et Biophysica Acta 1769(5-6): 393-398, 2007

Epigenetic modification in 4T1 mouse breast cancer model by artificial light at night and melatonin - the role of DNA-methyltransferase. Chronobiology International 2019: 1-15, 2019

Rice SUVH histone methyltransferase genes display specific functions in chromatin modification and retrotransposon repression. Molecular Plant 3(4): 773-782, 2010

Arabidopsis histone methyltransferase SET DOMAIN GROUP8 mediates induction of the jasmonate/ethylene pathway genes in plant defense response to necrotrophic fungi. Plant Physiology 154(3): 1403-1414, 2011

Arabidopsis Histone Methyltransferase Set Domain Group8 Mediates Induction of the Jasmonate/Ethylene Pathway Genes in Plant Defense Response to Necrotrophic Fungi. Plant Physiology 154(3): 1403-1414, 2010

Gfi1 coordinates epigenetic repression of p21Cip/WAF1 by recruitment of histone lysine methyltransferase G9a and histone deacetylase 1. Molecular and Cellular Biology 25(23): 10338-10351, 2005

Tubastatin A suppresses renal fibrosis via regulation of epigenetic histone modification and Smad3-dependent fibrotic genes. Vascular Pharmacology 72: 130-140, 2016