+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Transient dominant selection for the modification and generation of recombinant infectious bronchitis coronaviruses



Transient dominant selection for the modification and generation of recombinant infectious bronchitis coronaviruses



Methods in Molecular Biology 1282: 115-133



We have developed a reverse genetics system for the avian coronavirus infectious bronchitis virus (IBV) in which a full-length cDNA corresponding to the IBV genome is inserted into the vaccinia virus genome under the control of a T7 promoter sequence. Vaccinia virus as a vector for the full-length IBV cDNA has the advantage that modifications can be introduced into the IBV cDNA using homologous recombination, a method frequently used to insert and delete sequences from the vaccinia virus genome. Here, we describe the use of transient dominant selection as a method for introducing modifications into the IBV cDNA; this has been successfully used for the substitution of specific nucleotides, deletion of genomic regions, and the exchange of complete genes. Infectious recombinant IBVs are generated in situ following the transfection of vaccinia virus DNA, containing the modified IBV cDNA, into cells infected with a recombinant fowlpox virus expressing T7 DNA-dependent RNA polymerase.

Please choose payment method:






(PDF emailed within 1 workday: $29.90)

Accession: 059158676

Download citation: RISBibTeXText

PMID: 25720477


Related references

Transient dominant selection for the modification and generation of recombinant infectious bronchitis coronaviruses. Methods in Molecular Biology 454: 255-273, 2008

Generation of a recombinant avian coronavirus infectious bronchitis virus using transient dominant selection. Journal of Virological Methods 123(2): 203-211, 2005

Molecular characterization and pathogenicity of infectious bronchitis coronaviruses: complicated evolution and epidemiology in china caused by cocirculation of multiple types of infectious bronchitis coronaviruses. Intervirology 52(4): 223-234, 2009

Antigenic and genomic relatedness of turkey-origin coronaviruses, bovine coronaviruses, and infectious bronchitis virus of chickens. Avian diseases 45(4): 978-984, 2001

Coronaviruses from pheasants (Phasianus colchicus) are genetically closely related to coronaviruses of domestic fowl (infectious bronchitis virus) and turkeys. Avian Pathology 31(1): 81-93, 2002

Transient dominant selection of recombinant vaccinia viruses. Journal of Virology 64(6): 3108-3111, 1990

Recombinant duck enteritis viruses expressing major structural proteins of the infectious bronchitis virus provide protection against infectious bronchitis in chickens. Antiviral Research 130: 19-26, 2016

Identification of the avian infectious bronchitis coronaviruses with mutations in gene 3. Gene 412(1-2): 12-25, 2008

Recombinant infectious bronchitis viruses expressing heterologous S1 subunits: potential for a new generation of vaccines that replicate in Vero cells. Journal of General Virology 99(12): 1681-1685, 2018

Generation of long flavivirus expression cassettes by in vivo recombination and transient dominant selection. Gene (amsterdam). 149(2): 201, 1994

An overview of avian coronaviruses (infectious bronchitis) and their antigenic assessment with monoclonal antibodies. Proceedings of Western Poultry Disease Conference: 4th) 8-9, 1985

Comparative analyses of the nucleocapsid genes of several strains of infectious bronchitis virus and other coronaviruses. Virus Research. 25(3): 213-222, 1992

Comparative analyses of the nucleocapsid genes of several strains of infectious bronchitis virus and other coronaviruses. Comparative Haematology International 3(3): 213-222, 1993

Identification of a novel linear B-cell epitope in the M protein of avian infectious bronchitis coronaviruses. Journal of Microbiology 47(5): 589-599, 2009

A recombinant fowl adenovirus expressing the S1 gene of infectious bronchitis virus protects against challenge with infectious bronchitis virus. Vaccine 21(21/22): 2730-2736, 2003