+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Ubiquitin-specific proteases are differentially expressed throughout the Schistosoma mansoni life cycle



Ubiquitin-specific proteases are differentially expressed throughout the Schistosoma mansoni life cycle



Parasites and Vectors 8: 349



The ubiquitination process can be reversed by deubiquitinating enzymes (DUBs). These proteases are involved in ubiquitin processing, in the recovery of modified ubiquitin trapped in inactive forms, and in the recycling of ubiquitin monomers from polyubiquitinated chains. The diversity of DUB functions is illustrated by their number and variety of their catalytic domains with specific 3D architectures. DUBs can be divided into five subclasses: ubiquitin C-terminal hydrolases (UCHs), ubiquitin-specific proteases (USPs or UBPs), ovarian tumour proteases (OTUs), Machado-Joseph disease proteases (MJDs) and JAB1/MPN/Mov34 metalloenzymes (JAMMs). Considering the role that the ubiquitin-proteasome system has been shown to play during the development of Schistosoma mansoni, our main goal was to identify and characterize SmUSPs. Here, we showed the identification of putative ubiquitin-specific proteases using bioinformatic approaches. We also evaluated the gene expression profile of representative USP family members using qRT-PCR. We reported 17 USP family members in S. mansoni that present a conservation of UCH domains. Furthermore, the putative SmUSP transcripts analysed were detected in all investigated stages, showing distinct expression during S. mansoni development. The SmUSPs exhibiting high expression profiles were SmUSP7, SmUSP8, SmUSP9x and SmUSP24. S. mansoni USPs showed changes in expression levels for different life cycle stages indicating their involvement in cellular processes required for S. mansoni development. These data will serve as a basis for future functional studies of USPs in this parasite.

Please choose payment method:






(PDF emailed within 0-6 h: $19.90)

Accession: 059188839

Download citation: RISBibTeXText

PMID: 26112833

DOI: 10.1186/s13071-015-0957-4


Related references

Cloning of the SmSPO-1 gene preferentially expressed in sporocyst during the life cycle of the parasitic helminth Schistosoma mansoni. Biochimica et Biophysica Acta, Molecular Basis of Disease 1453(3): 412-416, 1999

Epitopes expressed on very low Mr Schistosoma mansoni adult tegumental antigens conform to a general pattern of life-cycle cross-reactivity. Parasitology 100 Pt 1: 73-81, 1990

Different forms of the mRNA encoding the heat-shock transcription factor are expressed during the life cycle of the parasitic helminth Schistosoma mansoni. European Journal of Biochemistry 253(2): 390-398, 1998

Stage-specific expression of the mRNA encoding a 14-3-3 protein during the life cycle of Schistosoma mansoni. Molecular and Biochemical Parasitology 73(1-2): 275-278, 1995

Stage-specific alternative splicing of the heat-shock transcription factor during the life-cycle of Schistosoma mansoni. Parasitology 129(Pt 5): 587-596, 2004

An atlas for Schistosoma mansoni organs and life-cycle stages using cell type-specific markers and confocal microscopy. Plos Neglected Tropical Diseases 5(3): E1009, 2011

Schistosoma mansoni proteases Sm31 (cathepsin B) and Sm32 (legumain) are expressed in the cecum and protonephridia of cercariae. Journal of Parasitology 87(5): 1218-1221, 2001

Observations on the life-cycle of Schistosoma mansoni in the laboratory, with a discussion on the snail vectors of S. mansoni and S. haematobium. Ann Trop Med And Parasitol 41(2): 173-177, 1947

Isolation of cDNA clones for differentially expressed genes of the human parasite Schistosoma mansoni. Proceedings of the National Academy of Sciences of the United States of America 83(15): 5534-5538, 1986

Dolichol phosphate mannose synthase is differentially expressed in male and female worms of Schistosoma mansoni. Comparative biochemistry and physiology Part B Biochemistry and molecular biology 131(3): 465-474, 2002

Screening the Schistosoma mansoni transcriptome for genes differentially expressed in the schistosomulum stage in search for vaccine candidates. Parasitology Research 108(1): 123-135, 2011

Transcriptional Profile and Structural Conservation of SUMO-Specific Proteases in Schistosoma mansoni. Journal of Parasitology Research 2012: 480824, 2012

The proteasome-ubiquitin pathway in the Schistosoma mansoni egg has development- and morphology-specific characteristics. Molecular and Biochemical Parasitology 175(2): 118-125, 2011

A The molluscan phase of the life cycle of Schistosoma mansoni. Journal of Parasitology Urbana 20: 131-132, 1933

Demonstra-tion of life cycle of Schistosoma mansoni. Transactions of the Royal Society of Tropical Medicine and Hygiene 45: 303-306, 1951