+ Site Statistics
References:
52,572,879
Abstracts:
28,705,754
PMIDs:
27,750,366
DOIs:
25,464,004
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Variability in growth/no growth boundaries of 188 different Escherichia coli strains reveals that approximately 75% have a higher growth probability under low pH conditions than E. coli O157:H7 strain ATCC 43888



Variability in growth/no growth boundaries of 188 different Escherichia coli strains reveals that approximately 75% have a higher growth probability under low pH conditions than E. coli O157:H7 strain ATCC 43888



Food Microbiology 45(Pt B): 222-230



This study investigated the variation in growth/no growth boundaries of 188 Escherichia coli strains. Experiments were conducted in Luria-Bertani media under 36 combinations of lactic acid (LA) (0 and 25 mM), pH (3.8, 3.9, 4.0, 4.1, 4.2 and 4.3 for 0 mM LA and 4.3, 4.4, 4.5, 4.6, 4.7 and 4.8 for 25 mM LA) and temperature (20, 25 and 30 °C). After 3 days of incubation, growth was monitored through optical density measurements. For each strain, a so-called purposeful selection approach was used to fit a logistic regression model that adequately predicted the likelihood for growth. Further, to assess the growth/no growth variability for all the strains at once, a generalized linear mixed model was fitted to the data. Strain was fitted as a fixed factor and replicate as a random blocking factor. E. coli O157:H7 strain ATCC 43888 was used as reference strain allowing a comparison with the other strains. Out of the 188 strains tested, 140 strains (∼75%) presented a significantly higher probability of growth under low pH conditions than the O157:H7 strain ATCC 43888, whereas 20 strains (∼11%) showed a significantly lower probability of growth under high pH conditions.

Please choose payment method:






(PDF emailed within 0-6 h: $19.90)

Accession: 059233200

Download citation: RISBibTeXText

PMID: 25500388

DOI: 10.1016/j.fm.2014.06.024


Related references

Growth of strains of the major non-O157 Shiga toxin-producing Escherichia coli serogroups is not different from growth of Escherichia coli O157:H7 in neutral broth (pH 7.4) and acidified broth (pH 5.6) at 10°C. Journal of Food Protection 77(9): 1617-1623, 2015

Low variability of growth parameters among six O157:H7 and non-O157:H7 Escherichia coli strains. Journal of Food Protection 77(11): 1988-1991, 2015

Dna synthesis during the cell division cycle of escherichia coli a comparison of escherichia coli strain b r escherichia coli k 12 and escherichia coli 15t under conditions of slow growth. Abstracts of the Annual Meeting of the American Society for Microbiology 73: 69, 1973

Stress-Induced Evolution of Heat Resistance and Resuscitation Speed in Escherichia coli O157:H7 ATCC 43888. Applied and Environmental Microbiology 82(22): 6656-6663, 2016

Thermotolerance of Escherichia coli O157:H7 ATCC 43,894, Escherichia coli B, and an rpoS-deficient mutant of Escherichia coli O157:H7 ATCC 43,895 following exposure to 1.5% acetic acid. Journal of Food Protection 61(9): 1184-1186, 1998

Effect of Tannins on the In Vitro Growth of Escherichia coli O157:H7 and In Vivo Growth of Generic Escherichia coli Excreted from Steers. Journal of Food Protection 70(3): 543-550, 2007

Effect of tannins on the in vitro growth of Escherichia coli O157 : H7 and in vivo growth of generic Escherichia coli excreted from steers. Journal of Food Protection 70(3): 543-550, 2007

Precolonized human commensal Escherichia coli strains serve as a barrier to E. coli O157:H7 growth in the streptomycin-treated mouse intestine. Infection and Immunity 77(7): 2876-2886, 2009

Optimal growth temperature of O157 and non-O157 Escherichia coli strains. Letters in Applied Microbiology 33(5): 352-356, 2001

Growth of Escherichia coli O157:H7, Non-O157 Shiga Toxin-Producing Escherichia coli , and Salmonella in Water and Hydroponic Fertilizer Solutions. Journal of Food Protection 79(12): 2179-2183, 2017

Morphological changes to Escherichia coli O157:H7, commensal E. coli and Salmonella spp in response to marginal growth conditions, with special reference to mildly stressing temperatures. Science Progress 86(Pt 1-2): 103-113, 2003

Role of glucose in enhancing the temperature-dependent growth inhibition of Escherichia coli O157:H7 ATCC 43895 by a Pseudomonas sp. Applied and Environmental Microbiology 68(5): 2600-2604, 2002

Hybridization of strains of Escherichia coli O157 with probes derived from the eaeA gene of enteropathogenic E. coli and the eaeA homolog from a Vero cytotoxin-producing strain of E. coli O157. Journal of Clinical Microbiology 32(4): 897-902, 1994

Enumeration of sublethally injured Escherichia coli O157:H7 ATCC 43895 and Escherichia coli strain B-41560 using selective agar overlays versus commercial methods. Journal of Food Protection 76(4): 674-679, 2013

Growth of Escherichia coli O157 in poorly fermented laboratory silage: a possible environmental dimension in the epidemiology of E. coli O157. Letters in Applied Microbiology 30(2): 118-121, 2000