+ Site Statistics
References:
54,258,434
Abstracts:
29,560,870
PMIDs:
28,072,757
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

iTRAQ-based quantitative proteomic analysis reveals proteomic changes in leaves of cultivated tobacco (Nicotiana tabacum) in response to drought stress



iTRAQ-based quantitative proteomic analysis reveals proteomic changes in leaves of cultivated tobacco (Nicotiana tabacum) in response to drought stress



Biochemical and Biophysical Research Communications 469(3): 768-775



Drought is one of the most severe forms of abiotic stresses that threaten the survival of plants, including crops. In turn, plants dramatically change their physiology to increase drought tolerance, including reconfiguration of proteomes. Here, we studied drought-induced proteomic changes in leaves of cultivated tobacco (Nicotiana tabacum), a solanaceous plant, using the isobaric tags for relative and absolute quantitation (iTRAQ)-based protein labeling technology. Of identified 5570 proteins totally, drought treatment increased and decreased abundance of 260 and 206 proteins, respectively, compared with control condition. Most of these differentially regulated proteins are involved in photosynthesis, metabolism, and stress and defense. Although abscisic acid (ABA) levels greatly increased in drought-treated tobacco leaves, abundance of detected ABA biosynthetic enzymes showed no obvious changes. In contrast, heat shock proteins (HSPs), thioredoxins, ascorbate-, glutathione-, and hydrogen peroxide (H2O2)-related proteins were up- or down-regulated in drought-treated tobacco leaves, suggesting that chaperones and redox signaling are important for tobacco tolerance to drought, and it is likely that redox-induced posttranslational modifications play an important role in modulating protein activity. This study not only provides a comprehensive dataset on overall protein changes in drought-treated tobacco leaves, but also shed light on the mechanism by which solanaceous plants adapt to drought stress.

Please choose payment method:






(PDF emailed within 0-6 h: $19.90)

Accession: 059286544

Download citation: RISBibTeXText

PMID: 26692494

DOI: 10.1016/j.bbrc.2015.11.133


Related references

iTRAQ-Based Quantitative Proteomic Analysis of Cotton Roots and Leaves Reveals Pathways Associated with Salt Stress. Plos One 11(2): E0148487, 2016

Investigating the proteomic expression profile of tobacco (Nicotiana tabacum) leaves during four growth stages using the iTRAQ method. Analytical and Bioanalytical Chemistry 2018, 2018

iTRAQ-based quantitative proteomic analysis reveals proteomic changes in three fenoxaprop-P-ethyl-resistant Beckmannia syzigachne biotypes with differing ACCase mutations. Journal of Proteomics 160: 47-54, 2017

iTRAQ-based quantitative proteomic analysis reveals new metabolic pathways responding to chilling stress in maize seedlings. Journal of Proteomics 146: 14-24, 2017

iTRAQ-based quantitative proteomic analysis reveals new metabolic pathways of wheat seedling growth under hydrogen peroxide stress. Proteomics 13(20): 3046-3058, 2014

iTRAQ-based quantitative proteomic analysis of wheat roots in response to salt stress. Proteomics 17(8): 1600265, 2017

iTRAQ-Based Quantitative Proteomic Analysis Reveals Changes in Metabolite Biosynthesis in Monascus purpureus in Response to a Low-Frequency Magnetic Field. Toxins 10(11), 2018

iTRAQ-based quantitative proteomic analysis of Takifugu fasciatus liver in response to low-temperature stress. Journal of Proteomics 2019, 2019

iTRAQ-based quantitative proteomic analysis of Pseudomonas aeruginosa SJTD-1: A global response to n-octadecane induced stress. Journal of Proteomics 123: 14-28, 2016

Gel-based and gel-free proteomic analysis of Nicotiana tabacum trichomes identifies proteins involved in secondary metabolism and in the (a)biotic stress response. Proteomics 11(3): 440-454, 2011

Differential proteomic analysis of grapevine leaves by iTRAQ reveals responses to heat stress and subsequent recovery. Bmc Plant Biology 14: 110, 2014

iTRAQ-based quantitative proteomic analysis of cultivated Pseudostellaria heterophylla and its wild-type. Journal of Proteomics 139: 13-25, 2017

iTRAQ-based quantitative proteomic analysis identified Eno1 as a cadmium stress response gene in Propsilocerus akamusi (Tokunaga) hemolymph. Ecotoxicology and Environmental Safety 165: 126-135, 2018

iTRAQ-based quantitative proteomic analysis reveals the role of the tonoplast in fruit senescence. Journal of Proteomics 146: 80-89, 2017

iTRAQ-based quantitative proteomic analysis reveals dynamic changes during daylily flower senescence. Planta 248(4): 859-873, 2018