Angiogenenic effects of BpLec, a C-type lectin isolated from Bothrops pauloensis snake venom
Castanheira, L.íc.E.; Lopes, D.S.; Gimenes, S.N.C.; Deconte, S.R.; Ferreira, B.A.ôn.; Alves, P.T.; Filho, L.R.G.; Tomiosso, T.C.; Rodrigues, R.S.; Yoneyama, K.A.G.; Araújo, F.d.A.; Rodrigues, V.d.M.
International Journal of Biological Macromolecules 102: 153-161
ISSN/ISBN: 1879-0003 PMID: 28392382 DOI: 10.1016/j.ijbiomac.2017.04.012
The present work reports the effects of a C-type lectin (BpLec) isolated from Bothrops pauloensis snake venom upon in vitro and in vivo angiogenesis models. Initially, we noted that BpLec was not cytotoxic to endothelial cells (tEnd) in doses up to 40μg/mL, but lower doses (2.5μg/mL, 5μg/mL, 10μg/mL and 20μg/mL) reduced tEnd cells adhesion to some extracellular matrix proteins and inhibited the in vitro vessel formation in Matrigel assay stimulated by bFGF. β-galactosides (d-lactose, N-acetyl-d-galactosamine and d-galactose) at 400mM reversed the effect of BpLec on tEnd cells adhesion, whereas d-galactose (400mM) partially reversed BpLec property of inhibiting vessel formation by tEnd cells in Matrigel. In vivo assays showed that BpLec increased hemoglobin content and capillary vessels number in polyether-polyurethane sponge discs subcutaneously implanted into dorsal skin mice. Additionally, BpLec also reduced collagen deposition and did not induce a pro-inflammatory response, as demonstrated by the decreased the secretion of some inflammatory cytokines, whereas myeloperoxidase (MPO) and N-acetylglucosaminidase (NAG) activities were not altered by BpLec. Taken together, our results indicate that BpLec might represent an interesting angiogenesis and inflammatory modulator that could also be used for searching possible therapeutic targets involved in these processes.