+ Site Statistics
+ Search Articles
+ Subscribe to Site Feeds
EurekaMag Most Shared ContentMost Shared
EurekaMag PDF Full Text ContentPDF Full Text
+ PDF Full Text
Request PDF Full TextRequest PDF Full Text
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter
Follow on LinkedInFollow on LinkedIn

+ Translate

A Novel Restraint Device for Injection of Galleria mellonella Larvae that Minimizes the Risk of Accidental Operator Needle Stick Injury

A Novel Restraint Device for Injection of Galleria mellonella Larvae that Minimizes the Risk of Accidental Operator Needle Stick Injury

Frontiers in Cellular and Infection Microbiology 7: 99-99

Larvae of the insect Galleria mellonella are increasingly being used for studying pathogenic microbes and their virulence mechanisms, and as a rapid model for screening novel antimicrobial agents. The larvae (waxworms) are most frequently infected by injection of pathogenic organisms into the haemocoel through the insect's prolegs. The mostly widely used method for restraining the waxworms for injection is by grasping them between the operator's fingers, which puts the operator at risk of needle stick injury, an important consideration when working with highly pathogenic and/or drug-resistant microorganisms. While use of a stab proof glove can reduce this risk of injury, it does so at the loss of manual dexterity and speed, resulting in a more labor-intensive, and cumbersome assay. We describe a simple cost effective device (the so-called "Galleria Grabber") for restraining waxworms for injection that keeps the operator's fingers clear of the needle thus reducing the risk of injury.

(PDF emailed within 0-6 h: $19.90)

Accession: 059373522

Download citation: RISBibTeXText

PMID: 28401069

DOI: 10.3389/fcimb.2017.00099

Related references

Injury induced molting in Galleria mellonella larvae. Biological Bulletin 142(2): 281-292, 1972

Accidental needle-stick injury. British Dental Journal 184(11): 525-525, 1998

Effects of injury to the neuroendocrine system of last-instar larvae of Galleria mellonella. Journal of Insect Physiology 32.12: 1011-1017, 1986

Peculiarities of the response reaction of the immunity system of larvae of Galleria mellonella L. to the injection of heterogenous proteins. Acta Parasitologica Lituanica 15: 93-97, 1977

Comparative levels of lysozyme activity in larvae and pupae of Galleria mellonella after particulate and soluble materials injection. Cytobios 26(103-104): 203-219, 1979

The prevention of nosocomial infections after an accidental needle-stick injury. Deutsche Medizinische Wochenschrift 117(13): 515-517, 1992

Exoproteinase of the type InA in Galleria mellonella larvae infected with Serratia marcescens Proteinaza typu InA w gasienicach Galleria mellonella zakazonych Serratia marcescens. Annales Universitatis Mariae Curie-Sklodowska Sectio C Biologia, 51: 7-12, 1996

Necrotizing fasciitis due to group A streptococcus after an accidental needle-stick injury. New England Journal of Medicine 337(23): 1699-1699, 1997

A Simple Method of Preventing Needle Stick Type Injury to the Operator's Finger While Performing Intermaxillary Fixation. Journal of Maxillofacial and Oral Surgery 13(2): 215-216, 2014

Anti-stick device for the safe handling of an injection needle. Official Gazette of the United States Patent & Trademark Office Patents 1277(3), Dec 16, 2003

Reducing the risk of needle-stick injury during orbital fine-needle aspiration biopsy. Canadian Journal of Ophthalmology. Journal Canadien D'ophtalmologie 31(3): 124-125, 1996

The Prevalence of Accidental Needle Stick Injury and their Reporting among Healthcare Workers in Orthopaedic Wards in General Hospital Melaka, Malaysia. Malaysian Orthopaedic Journal 8(2): 6-13, 2014

Histology and modalities of secretion of the mid gut of pieris brassicae and galleria mellonella chronology of epithelial renewals during larval molts and nymphal age of galleria mellonella. Annales de Zoologie Ecologie Animale 2(1): 25-50, 1970

Fine-needle aspiration anchor. A simple device to prevent needle-stick injury at fine-needle aspiration. Archives of Pathology & Laboratory Medicine 117(10): 1047-1049, 1993

Distribution of first and second instar larvae of Pseudogonia rufifrons Wied. in larvae and eopupae of Galleria mellonella L. Bollettino dell'Istituto di entomologia dell'Universita degli studi di Bologna0(40): 151-166, 1986