+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

A polygalacturonase localized in the Golgi apparatus in Pisum sativum



A polygalacturonase localized in the Golgi apparatus in Pisum sativum



Journal of Biochemistry 162(3): 193-201



Pectin is a plant cell wall constituent that is mainly composed of polygalacturonic acid (PGA), a linear α1,4-d-galacturonic acid (GalUA) backbone. Polygalacturonase (PG) hydrolyzes the α1,4-linkages in PGA. Nearly all plant PGs identified thus far are secreted as soluble proteins. Here we describe the microsomal PG activity in pea (Pisum sativum) epicotyls and present biochemical evidence that it was localized to the Golgi apparatus, where pectins are biosynthesized. The microsomal PG was purified, and it was enzymatically characterized. The purified enzyme showed maximum activity towards pyridylaminated oligogalacturonic acids with six degrees of polymerization (PA-GalUA6), with a Km value of 11 μM for PA-GalUA6. The substrate preference of the enzyme was complementary to that of PGA synthase. The main PG activity in microsomes was detected in the Golgi fraction by sucrose density gradient ultracentrifugation. The activity of the microsomal PG was lower in rapidly growing epicotyls, in contrast to the high expression of PGA synthase. The role of this PG in the regulation of pectin biosynthesis or plant growth is discussed.

Please choose payment method:






(PDF emailed within 0-6 h: $19.90)

Accession: 059387217

Download citation: RISBibTeXText

PMID: 28338792

DOI: 10.1093/jb/mvx014


Related references

Golgi apparatus secretion in plasmolyzed pisum sativum. Protoplasma 90(3-4): 369-379, 1976

Golgi Apparatus in Tip Cells of Pisum sativum Primary Roots. Transactions of the American Microscopical Society 87(4): 516-521, 1968

Distribution of xylosyltransferases and glucuronyltransferase within the Golgi apparatus in etiolated pea (Pisum sativum L.) epicotyls. Journal of Experimental Botany 48(311): 1209-1214, 1997

Golgi apparatus in tip cells of pisum sativum d primary roots inst electron microscope. Transactions of the American Microscopical Society 87(4): 516-520, 1968

Differential distribution of a glucuronyltransferase, involved in glucuronoxylan synthesis, within the Golgi apparatus of pea (Pisum sativum var. Alaska). Biochemical Journal 277: 653-658, 1991

Differential distribution of a glucuronyltransferase involved in glucuronoxylan synthesis within the golgi apparatus of pea pisum sativum new variety cultivar alaska. Biochemical Journal 277(3): 653-658, 1991

Localization of pisum sativum agglutinin and lens culinaris agglutinin binding sites an approach to discriminate subsections of the golgi apparatus studies with differentiating and mature absorptive cells. European Journal of Cell Biology Suppl. (12): 26, 1985

Comparative studies on the nutritive value of 2 varieties of peas (Pisum sativum ssp. sativum and Pisum sativum ssp. medulare) in cattle and pigs. Agribiological Research 43(3): 225-233, 1990

A common peptide stretch among enzymes localized to the Golgi apparatus: structural similarity of Golgi-associated glycosyltransferases. Biochemical and Biophysical Research Communications 170(2): 879-882, 1990

The small GTP-binding protein Rho1p is localized on the Golgi apparatus and post-Golgi vesicles in Saccharomyces cerevisiae. Journal of Cell Biology 115(2): 309-319, 1991

Xyloglucan glucosyltransferase in Golgi membranes from Pisum sativum (pea). Biochemical Journal 294: 231-238, 1993

Transgenic peas (Pisum sativum) expressing polygalacturonase inhibiting protein from raspberry (Rubus idaeus) and stilbene synthase from grape (Vitis vinifera). Plant Cell Reports 25(11): 1166-1173, 2006

Effects of localized ultrasonic irradiation on pisum sativum roots. Environmental and Experimental Botany 18(4): 207-218, 1978

Golgi-localized KIAA0725p regulates membrane trafficking from the Golgi apparatus to the plasma membrane in mammalian cells. Febs Letters 584(21): 4389-4395, 2010

Golgi mediated vicilin accumulation in pea pisum sativum cotyledon cells is re directed by monensin and nigericin. Protoplasma 122(1-2): 91-97, 1984