+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

A Prospective Cohort Multicenter Study of Molecular Epidemiology and Phylogenomics of Staphylococcus aureus Bacteremia in Nine Latin American Countries

A Prospective Cohort Multicenter Study of Molecular Epidemiology and Phylogenomics of Staphylococcus aureus Bacteremia in Nine Latin American Countries

Antimicrobial Agents and ChemoTherapy 61(10)

Staphylococcus aureus is an important pathogen causing a spectrum of diseases ranging from mild skin and soft tissue infections to life-threatening conditions. Bloodstream infections are particularly important, and the treatment approach is complicated by the presence of methicillin-resistant S. aureus (MRSA) isolates. The emergence of new genetic lineages of MRSA has occurred in Latin America (LA) with the rise and dissemination of the community-associated USA300 Latin American variant (USA300-LV). Here, we prospectively characterized bloodstream MRSA recovered from selected hospitals in 9 Latin American countries. All isolates were typed by pulsed-field gel electrophoresis (PFGE) and subjected to antibiotic susceptibility testing. Whole-genome sequencing was performed on 96 MRSA representatives. MRSA represented 45% of all (1,185 S. aureus) isolates. The majority of MRSA isolates belonged to clonal cluster (CC) 5. In Colombia and Ecuador, most isolates (≥72%) belonged to the USA300-LV lineage (CC8). Phylogenetic reconstructions indicated that MRSA isolates from participating hospitals belonged to three major clades. Clade A grouped isolates with sequence type 5 (ST5), ST105, and ST1011 (mostly staphylococcal chromosomal cassette mec [SCCmec] I and II). Clade B included ST8, ST88, ST97, and ST72 strains (SCCmec IV, subtypes a, b, and c/E), and clade C grouped mostly Argentinian MRSA belonging to ST30. In summary, CC5 MRSA was prevalent in bloodstream infections in LA with the exception of Colombia and Ecuador, where USA300-LV is now the dominant lineage. Clonal replacement appears to be a common phenomenon, and continuous surveillance is crucial to identify changes in the molecular epidemiology of MRSA.

Please choose payment method:

(PDF emailed within 0-6 h: $19.90)

Accession: 059392167

Download citation: RISBibTeXText

PMID: 28760895

DOI: 10.1128/aac.00816-17

Related references

Prospective multicenter study of Staphylococcus aureus bacteremia - results from the "Invasive Staphylococcus aureus Infection Cohort" (INSTINCT) study FT Epidemiologie, Verlauf and Prognose der Staphylococcus-aureus-Bakteriamie - Erste Ergebnisse der INSTINCT-Kohorte. 2008

Epidemiology, course and prognosis of Staphylococcus aureus bacteremia--Preliminary results from the INSTINCT (INvasive STaphylococcus aureus INfection CohorT) cohort. Deutsche Medizinische Wochenschrift 133(8): 340-345, 2008

Molecular epidemiologic analysis of methicillin-resistant Staphylococcus aureus isolates from bacteremia and nasal colonization at 10 intensive care units: multicenter prospective study in Korea. Journal of Korean Medical Science 26(5): 604-611, 2011

Staphylococcus aureus bacteremia in immunosuppressed patients: a multicenter, retrospective cohort study. European Journal of Clinical Microbiology and Infectious Diseases 36(7): 1231-1241, 2017

Mortality predictors of Staphylococcus aureus bacteremia: a prospective multicenter study. Annals of Clinical Microbiology and Antimicrobials 15: 7, 2016

Outcome of staphylococcus aureus bacteremia in hemodialysis patients a prospective multicenter study. Abstracts of the Annual Meeting of the American Society for Microbiology 90: 17, 1990

Prospective, multicenter study of Staphylococcus aureus bacteremia Risk for endocarditis and methicillin-resistance. Clinical Infectious Diseases 25(2): 436, 1997

Staphylococcus aureus bacteremia: recurrence and the impact of antibiotic treatment in a prospective multicenter study. Medicine 82(5): 333-339, 2003

Use of Transthoracic Echocardiography in the Management of Low-Risk Staphylococcus aureus Bacteremia: Results From a Retrospective Multicenter Cohort Study. Jacc. Cardiovascular Imaging 8(8): 924-931, 2015

Mortality among critically ill patients with methicillin-resistant Staphylococcus aureus bacteremia: a multicenter cohort study in Colombia. Revista Panamericana de Salud Publica 32(5): 343-350, 2012

Staphylococcus aureus bloodstream infections in Latin America: results of a multinational prospective cohort study. Journal of Antimicrobial ChemoTherapy 73(1): 212-222, 2018

Methicillin susceptible Staphylococcus aureus bacteremia: epidemiology and prognostic factors. A prospective study 2000-2003. Revista Clinica Espanola 207(2): 57-63, 2007

A prospective cohort pilot study of the clinical and molecular epidemiology of Staphylococcus aureus in pregnant women at the time of group B streptococcal screening in a large urban medical center in Chicago, IL USA. Virulence 4(7): 654-658, 2013

Preventing Staphylococcus aureus bacteremia and sepsis in patients with Staphylococcus aureus colonization of intravascular catheters: a retrospective multicenter study and meta-analysis. Medicine 90(4): 284-288, 2011

A prospective multicenter study of Staphylococcus aureus bacteremia: incidence of endocarditis, risk factors for mortality, and clinical impact of methicillin resistance. Medicine 82(5): 322-332, 2003