+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Clonal Inheritance of a Diploid Nuclear Genome by a Hybrid Freshwater Minnow (Phoxinus Eos-Neogaeus, Pisces: Cyprinidae)



Clonal Inheritance of a Diploid Nuclear Genome by a Hybrid Freshwater Minnow (Phoxinus Eos-Neogaeus, Pisces: Cyprinidae)



Evolution; International Journal of Organic Evolution 44(4): 1052-1065



Hybrids between the minnows Phoxinus eos and Phoxinus neogaeus coexist with a population of P. eos in East Inlet Pond, Coos Co., New Hampshire. Chromosome counts and flow cytometric analysis of erythrocyte DNA indicate that these hybrids include diploids, triploids, and diploid-triploid mosaics. The mosaics have both diploid and triploid cells in their bodies, even within the same tissues. All three hybrid types are heterozygous at seven putative loci for which P. eos and P. neogaeus are fixed for different allozymes, indicating that the hybrids carry one eos and one neogaeus haploid genome. The diploid hybrids are therefore P. eos-neogaeus, whereas the triploids and mosaics are derived from P. eos-neogaeus but have an extra eos or neogaeus genome in all or some of their cells. Diploid, triploid, and mosaic hybrids accept tissue grafts from diploid hybrids, indicating that all individuals carry the identical eos-neogaeus diploid genome. Thus, one P. eos-neogaeus clone exists at East Inlet Pond. Grafts among the triploids and mosaics or from these individuals to diploid hybrids are rejected, indicating that the third genome is different in each triploid and mosaic individual. In this study, diploid and mosaic hybrids, carrying the clonal eos-neogaeus genome, were bred in the laboratory with males of P. eos or P. neogaeus. Both diploid and mosaic hybrids produced diploid, triploid, and mosaic offspring, revealing the source of the three hybrid types present at East Inlet Pond. These offspring accepted grafts from P. eos-neogaeus individuals, indicating that they all had inherited the identical eos-neogaeus genome. Most grafts among triploid and mosaic progeny, or from these individuals to their diploid broodmates, were rejected, indicating that the third genome was different in each triploid and mosaic (as was observed in the wild hybrids) and was contributed by sperm from males of P. eos or P. neogaeus. Diploid progeny are produced if sperm serves only to stimulate embryogenesis; triploid or mosaic progeny are produced if the sperm genome is incorporated. Although based on a mode of reproduction that by definition results in a genetically identical community of individuals, i.e., gynogenesis, reproduction in hybrid Phoxinus results in a variety of genetically distinct individuals by the incorporation of sperm into approximately 50% of the diploid ova produced.

Please choose payment method:






(PDF emailed within 0-6 h: $19.90)

Accession: 059512817

Download citation: RISBibTeXText

PMID: 28569023

DOI: 10.1111/j.1558-5646.1990.tb03825.x


Related references

Clonal inheritance of a diploid nuclear genome by a hybrid freshwater minnow phoxinus eos x phoxinus neogaeus pisces cyprinidae. Evolution 44(4): 1052-1065, 1990

Clonal inheritance of a diploid nuclear genome by a hybrid freshwater minnow Phoxinus eos-neogaeus, Pisces Cyprinidae. Evolution 44.4: 1052-1065, 1990

Diploidy and triploidy in the hybrid minnow,Phoxinus eos x Phoxinus neogaeus(Pisces: Cyprinidae). Cellular and Molecular Life Sciences 41(4): 505-507, 1985

Diploidy and triploidy in the hybrid minnow, Phoxinus eos x Phoxinus neogaeus (Pisces: Cyprinidae). Experientia (Basel): 414: 505-507, 1985

Clonal reproduction and polyploidy in unisexual hybrids of phoxinus eos and phoxinus neogaeus pisces cyprinidae. Copeia (2): 275-283, 1987

Allozyme analysis of the hybrid Phoxinus eos x Phoxinus neogaeus (Pisces: Cyprinidae) in Nebraska. Canadian Journal of Zoology 605: 968-973, 1982

Allozyme analysis of the hybrid Phoxinus eos Phoxinus neogaeus (Pisces: Cyprinidae) in Nebraska. Canadian Journal of Zoology 60(5): 968-973, 1982

Allozyme analysis of the hybrid phoxinus eos x phoxinus neogaeus pisces cyprinidae in nebraska usa. Canadian Journal of Zoology 60(5): 968-973, 1982

Laboratory development of the hybrid phoxinus eos x phoxinus neogaeus pisces cyprinidae. Experientia 44(3): 262-263, 1988

Multivariate analysis of the hybrid Phoxinus eos (times) Phoxinus neogaeus (Pisces: Cyprinidae): reevaluation of univariate analysis. Canadian Journal of Zoology 67: 37-41, 1989

Multivariate analysis of the hybrid phoxinus eos x phoxinus neogaeus pisces cyprinidae reevaluation of univariate analysis. Canadian Journal of Zoology 67(7): 1637-1641, 1989

Multivariate analysis of the hybrid Phoxinus eos Phoxinus neogaeus (Pisces: Cyprinidae): reevaluation of univariate analysis. Canadian Journal of Zoology 67(7): 1637-1641, 1989

Aclonal reproduction by polyploid members of the clonal hybrid species Phoxinus eos neogaeus (Cyprinidae). Copeia 1993(3): 650-660, 1993

Extreme clonal uniformity of Phoxinus eos/neogaeus gynogens (pisces: Cyprinidae) among variable habitats in northern Minnesota beaver ponds. Proceedings of the National Academy of Sciences of the United States of America 92(11): 5001-5005, 1995

Phenotypic Consequences of Genetic Variation in a Gynogenetic Complex of Phoxinus eos-neogaeus Clonal Fish (Pisces: Cyprinidae) Inhabiting a Heterogeneous Environment. Evolution 58(6): 61-73, 2004