EurekaMag
+ Most Popular
Cunninghamia lanceolata plantations in China
Mammalian lairs in paleo ecological studies and palynology
Studies on technological possibilities in utilization of anhydrous milk fat for production of recombined butter-like products
Should right-sided fibroelastomas be operated upon?
Large esophageal lipoma
Apoptosis in the mammalian thymus during normal histogenesis and under various in vitro and in vivo experimental conditions
Poissons characoides nouveaux ou non signales de l'Ilha do Bananal, Bresil
Desensitizing efficacy of Colgate Sensitive Maximum Strength and Fresh Mint Sensodyne dentifrices
Administration of fluid by subcutaneous infusion: revival of a forgotten method
Tundra mosquito control - an impossible dream?
Schizophrenia for primary care providers: how to contribute to the care of a vulnerable patient population
Geochemical pattern analysis; method of describing the Southeastern limestone regional aquifer system
Incidence of low birth weights in a hospital of Mexico City
Tabanidae
Graded management intensity of grassland systems for enhancing floristic diversity
Microbiology and biochemistry of cheese and fermented milk
The ember tetra: a new pygmy characid tetra from the Rio das Mortes, Brazil, Hyphessobrycon amandae sp. n. (Pisces, Characoidei)
Risk factors of contrast-induced nephropathy in patients after coronary artery intervention
Renovation of onsite domestic wastewater in a poorly drained soil
Observations of the propagation velocity and formation mechanism of burst fractures caused by gunshot
Systolic blood pressure in a population of infants in the first year of life: the Brompton study
Haematological studies in rats fed with metanil yellow
Studies on pasteurellosis. I. A new species of Pasteurella encountered in chronic fowl cholera
Dormancy breaking and germination of Acacia salicina Lindl. seeds
therapy of lupus nephritis. a two-year prospective study

Comparison of assays for the detection of West Nile virus antibodies in equine serum after natural infection or vaccination


Comparison of assays for the detection of West Nile virus antibodies in equine serum after natural infection or vaccination



Veterinary Immunology and Immunopathology 183: 1-6



ISSN/ISBN: 0165-2427

PMID: 28063471

DOI: 10.1016/j.vetimm.2016.10.015

West Nile virus (WNV) mainly infects birds, horses and humans. Outcomes of the infection range from mild uncharacteristic signs to fatal neurologic disease. The main objectives of the present study were to measure serum IgG and IgM antibodies in naturally exposed and vaccinated horses and to compare results of haemagglutination inhibition test (HIT), enzyme-linked immunosorbent assay (ELISA) and plaque reduction neutralisation test (PRNT). Altogether 224 animals were tested by HIT for WNV antibodies and 41 horses were simultaneously examined by ELISA and PRNT. After primary screening for WNV antibodies, horses were vaccinated. Samples were taken immediately before and 3-5 weeks after each vaccination. McNemar's chi-squared and percent agreement tests were used to detect concordance between HIT, ELISA and PRNT. Analyses by HIT confirmed the presence of WNV antibodies in 27/105 (26%) naturally exposed horses. Sera from 57/66 (86%) vaccinated animals were positive before the first booster and from 11/11 (100%) before the second booster. HIT was less sensitive for detecting IgG antibodies. We could detect postvaccination IgM in 13 cases with IgM antibody capture ELISA (MAC-ELISA) and in 7 cases with HIT. WNV is endemic in Hungary and regularly causes natural infections. Protective antibodies could not be measured in some of the cases 12 months after primary vaccinations; protection is more reliable after the first yearly booster. Based on our findings it was not possible to differentiate infected from recently vaccinated horses using MAC-ELISA. HIT cannot be used as a substitute for ELISA or PRNT when detecting IgG, but it proved to be a useful tool in this study to gain statistical information about the tendencies within a fixed population of horses.

Please choose payment method:






(PDF emailed within 0-6 h: $19.90)

Accession: 059527783

Download citation: RISBibTeXText

Related references

Comparison of assays for the detection of West Nile virus antibodies in chicken serum. Canadian Journal of Veterinary Research 67(2): 128-132, 2003

Performance of immunoglobulin G (IgG) and IgM enzyme-linked immunosorbent assays using a West Nile virus recombinant antigen (preM/E) for detection of West Nile virus- and other flavivirus-specific antibodies. Journal of Clinical Microbiology 42(10): 4641-4648, 2004

Epitope-blocking enzyme-linked immunosorbent assays for the detection of serum antibodies to west nile virus in multiple avian species. Journal of Clinical Microbiology 41(3): 1041-1047, 2003

Immunoglobulin M-capture enzyme-linked immunosorbent assay testing of cerebrospinal fluid and serum from horses exposed to west nile virus by vaccination or natural infection. Journal of Veterinary Internal Medicine 18(6): 866-870, 2004

A review on assays for detecting antibodies of West Nile virus infection. Zhonghua Liu Xing Bing Xue Za Zhi 30(9): 969-972, 2009

Detection of antibodies to West Nile virus in equine sera using microsphere immunoassay. Journal of Veterinary Diagnostic Investigation: Official Publication of the American Association of Veterinary Laboratory Diagnosticians Inc 18(4): 392-395, 2006

Monoclonal antibodies to equine IgM improve the sensitivity of West Nile virus-specific IgM detection in horses. Veterinary Immunology and Immunopathology 122(1-2): 46-56, 2008

Assays for detecting West Nile Virus antibodies in human serum, plasma, and cerebrospinal fluid. Clinical and Applied Immunology Reviews 5(1): 45-63, 2004

Studies on West Nile virus infection by means of fluorescent antibodies. n. Pathogenesis of West Nile virus infection in experimentally inoculated chicks. Arch Ges Virusforsch 12(4): 529-536, 1963

Epitope-blocking enzyme-linked immunosorbent assays for detection of west nile virus antibodies in domestic mammals. Journal of Clinical Microbiology 41(6): 2676-2679, 2003

Studies on West Nile infection by means of fluorescent antibodies. I. Pathogenesis of West Nile virus infection in experimentally inoculated suckling mice. Arch Ges Virusforsch 12(4): 514-528, 1963

A new competitive ELISA detects West Nile virus infection using monoclonal antibodies against the precursor-membrane protein of West Nile virus. Journal of Virological Methods 188(1-2): 132-138, 2013

The natural history of West Nile virus infection presenting with West Nile virus meningoencephalitis in a man with a prolonged illness: a case report. Journal of Medical Case Reports 5: 204, 2011

Development of serum antibodies in 4 cases of West Nile virus infection. Annales de l'Institut Pasteur 113(1): 29-36, 1967

The natural history of West Nile fever. II. Virological findings and the development of homologous and heterologous antibodies in West Nile infection in man. American Journal of Hygiene 66(3): 363-380, 1957