+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Impact of Built-up-Litter and Commercial Antimicrobials on Salmonella and Campylobacter Contamination of Broiler Carcasses Processed at a Pilot Mobile Poultry-Processing Unit

Impact of Built-up-Litter and Commercial Antimicrobials on Salmonella and Campylobacter Contamination of Broiler Carcasses Processed at a Pilot Mobile Poultry-Processing Unit

Frontiers in Veterinary Science 4: 88

The small-scale mobile poultry-processing unit (MPPU) produced raw poultry products are of particular food safety concern due to exemption of USDA poultry products inspection act. Limited studies reported the microbial quality and safety of MPPU-processed poultry carcasses. This study evaluated the Salmonella and Campylobacter prevalence in broiler ceca and on MPPU-processed carcasses and efficacy of commercial antimicrobials against Campylobacter jejuni on broilers. In study I, straight-run Hubbard × Cobb broilers (147) were reared for 38 days on clean-shavings (CS, 75) or built-up-litter (BUL, 72) and processed at an MPPU. Aerobic plate counts (APCs), coliforms, Escherichia coli, and yeast/molds (Y/M) of carcasses were analyzed on petrifilms. Ceca and carcass samples underwent microbial analyses for Salmonella and Campylobacter spp. using the modified USDA method and confirmed by API-20e test (Salmonella), latex agglutination immunoassay (Campylobacter), and Gram staining (Campylobacter). Quantitative polymerase chain reaction (CadF gene) identified the prevalence of C. jejuni and Campylobacter coli in ceca and on carcasses. In study II, fresh chilled broiler carcasses were spot inoculated with C. jejuni (4.5 log10 CFU/mL) and then undipped, or dipped into peroxyacetic acid (PAA) (1,000 ppm), lactic acid (5%), lactic and citric acid blend (2.5%), sodium hypochlorite (69 ppm), or a H2O2-PAA mix (SaniDate® 5.0, 0.25%) for 30 s. Surviving C. jejuni was recovered onto Brucella agar. APCs, coliforms, and E. coli populations were similar (P > 0.05) on CS and BUL carcasses. Carcasses of broilers raised on BUL contained a greater (P < 0.05) Y/M population (2.2 log10 CFU/mL) than those reared on CS (1.8 log10 CFU/mL). Salmonella was not detected in any ceca samples, whereas 2.8% of the carcasses from BUL were present with Salmonella. Prevalence of Campylobacter spp., C. jejuni was lower (P < 0.05), and C. coli was similar (P > 0.05) in CS-treated ceca than BUL samples. Prevalence of Campylobacter spp., C. jejuni, and C. coli was not different (P > 0.05) on CS- and BUL-treated carcasses. All antimicrobials reduced C. jejuni by 1.2-2.0 log CFU/mL on carcasses compared with controls. Hence, raising broilers on CS and applying post-chilling antimicrobial treatment can reduce Salmonella and Campylobacter on MPPU-processed broiler carcasses.

Please choose payment method:

(PDF emailed within 0-6 h: $19.90)

Accession: 059835588

Download citation: RISBibTeXText

PMID: 28649571

DOI: 10.3389/fvets.2017.00088

Related references

Incidence of Salmonella on broiler carcasses and livers in a poultry slaughterhouse - impact of processing procedures on the contamination. Microbiologie, Aliments, Nutrition 12(1): 75-85, 1994

Evaluation of logistic processing to reduce cross-contamination of commercial broiler carcasses with Campylobacter spp. Journal of Food Protection 70(11): 2549-2554, 2007

Evaluation of Logistic Processing To Reduce Cross-Contamination of Commercial Broiler Carcasses with Campylobacter spp. Journal of Food Protection 70(11): 2549-2554, 2007

A pilot study on post-evisceration contamination of broiler carcasses and ready-to-sell livers and intestines (mala) with Campylobacter jejuni and Campylobacter coli in a high-throughput South African poultry abattoir. Journal of the South African Veterinary Association 77(3): 114-119, 2006

The Impact of Commercial Processing Procedures on the Bacterial Contamination and Cross-Contamination of Broiler Carcasses. Journal of Food Protection 53(3): 202-204, 2019

The impact of commercial processing procedures on the bacterial contamination and cross contamination of broiler carcasses. Journal of Food Protection 53(3): 202-204, 207, 1990

Microbiological quality of broiler chicken carcasses processed hygienically in a small scale poultry processing unit. Journal of food quality 24(3): 249-259, 2001

Salmonella contamination in a commercial poultry (broiler) processing operation. Poultry Science 53(2): 814-821, 1974

Campylobacter contamination of broiler caeca and carcasses at the slaughterhouse and correlation with Salmonella contamination. Food Microbiology 28(5): 862-868, 2011

Salmonella and Campylobacter prevalence and concentration on pasture-raised broilers processed on-farm, in a Mobile Processing Unit, and at small USDA-inspected facilities. Food Control 34(1): 177-182, 2013

Poultry flocks as a source of Campylobacter contamination of broiler carcasses. Polish Journal of Veterinary Sciences 18(1): 101-106, 2015

Impact of transport crate reuse and of catching and processing on Campylobacter and Salmonella contamination of broiler chickens. Applied and Environmental Microbiology 68(2): 713-719, 2002

Effect of fecal contamination and cross-contamination on numbers of coliform, Escherichia coli, Campylobacter, and Salmonella on immersion-chilled broiler carcasses. Journal of Food Protection 68(7): 1340-1345, 2005

Salmonella and Campylobacter reduction and quality characteristics of poultry carcasses treated with various antimicrobials in a post-chill immersion tank. International Journal of Food Microbiology 165(3): 281-286, 2013

Campylobacter, Salmonella and E. coli on broiler carcasses from commercial plants under HIMP inspection. Poultry Science 87: 134-134, 2008