EurekaMag
+ Most Popular
Cunninghamia lanceolata plantations in China
Mammalian lairs in paleo ecological studies and palynology
Studies on technological possibilities in utilization of anhydrous milk fat for production of recombined butter-like products
Should right-sided fibroelastomas be operated upon?
Large esophageal lipoma
Apoptosis in the mammalian thymus during normal histogenesis and under various in vitro and in vivo experimental conditions
Poissons characoides nouveaux ou non signales de l'Ilha do Bananal, Bresil
Desensitizing efficacy of Colgate Sensitive Maximum Strength and Fresh Mint Sensodyne dentifrices
Administration of fluid by subcutaneous infusion: revival of a forgotten method
Tundra mosquito control - an impossible dream?
Schizophrenia for primary care providers: how to contribute to the care of a vulnerable patient population
Geochemical pattern analysis; method of describing the Southeastern limestone regional aquifer system
Incidence of low birth weights in a hospital of Mexico City
Tabanidae
Graded management intensity of grassland systems for enhancing floristic diversity
Microbiology and biochemistry of cheese and fermented milk
The ember tetra: a new pygmy characid tetra from the Rio das Mortes, Brazil, Hyphessobrycon amandae sp. n. (Pisces, Characoidei)
Risk factors of contrast-induced nephropathy in patients after coronary artery intervention
Renovation of onsite domestic wastewater in a poorly drained soil
Observations of the propagation velocity and formation mechanism of burst fractures caused by gunshot
Systolic blood pressure in a population of infants in the first year of life: the Brompton study
Haematological studies in rats fed with metanil yellow
Studies on pasteurellosis. I. A new species of Pasteurella encountered in chronic fowl cholera
Dormancy breaking and germination of Acacia salicina Lindl. seeds
therapy of lupus nephritis. a two-year prospective study

Impact of Zeatin and Thidiazuron on Phenols and Flavonoids Accumulation in Callus Cultures of Gardenia (Gardenia jasminoides)


Impact of Zeatin and Thidiazuron on Phenols and Flavonoids Accumulation in Callus Cultures of Gardenia (Gardenia jasminoides)



Pakistan Journal of Biological Sciences: Pjbs 20(7): 328-335



ISSN/ISBN: 1028-8880

PMID: 29023064

Gardenia (Gardenia jasminoides) has many pharmacological actions such as anti-inflammatory, antioxidant and fibrolytic activities and cytotoxic effects, etc. This study was conducted to recognize the effect of zeatin and thidiazuron (TDZ) on callus proliferation, total phenolic content, total flavonoids and DPPH scavenging activity of gardenia callus cultures. Calli were cultured on Murashige and Skoog (MS) medium supplement with different concentrations (2, 4 or 6 mg L-1) of zeatin or TDZ individually as well as combination of 2 mg L-1 zeatin+4 mg L-1 TDZ. Cultures contained 4 mg L-1 TDZ gave the highest callus fresh weight followed by those contained 2 mg L-1 zeatin then that cultured on 4 mg L-1 zeatin. Data reported as Mean±Standard Deviation (SD). Data were subjected to one-way analysis of variance (p< 0.05). Results were processed by Excel (2010) and SPSS Version 17.0. It was found that callus growing on medium supplemented with 4 mg L-1 zeatin gave the maximum value (14.93%) of yield extract. Callus cultured on 4 mg L-1 zeatin recorded the maximum total phenol (268.33 mg GAE/100 g FW of callus) and total flavonoids (2703.33 μg QE/100 g FW of callus) accumulation. The antioxidant activity of each extract was determined through DPPH radical scavenging activity. Callus cultured on 4 mg L-1 TDZ showed the highest antioxidant activity then those cultured on 4 mg L-1 zeatin. The HPLC analysis for phenolic acids showed that chlorogenic acid, rosmarinic acid and cinnamic reached their highest contents with callus cultured on 4 mg L-1 TDZ (123.24, 322.14 and 278.22 μg g-1, respectively). Regarding flavonoids and using HPLC analysis, rutin, apigenin-7-glucoside and kaempferol were detected. Callus cultured with 4 mg L-1 TDZ gave the highest rutin and kaempferol contents (287.76 and 10.38 μg g-1, respectively). However, apigenin-7-glucoside was detected with high content (129.86 μg g-1) in callus culture with 4 mg L-1 Zeatin. The HPLC analysis recommended that TDZ is more effective in accumulation of individual phenolic and flavonoid than Zeatin. The present study provided a useful system for further study on in vitro culture of G. jasminoides as alternative and new source for important secondary products.

Please choose payment method:






(PDF emailed within 1 workday: $29.90)

Accession: 059839690

Download citation: RISBibTeXText

Related references

Callus induction and adventitious shoot regeneration of gardenia (Gardenia jasminoides Ellis) leaf explants. Scientia Horticulturae 72(3/4): 171-178, 1998

The influence of cytokinins and gibberellic acid on gardenia gardenia jasminoides tissue cultures. Scientia Horticulturae 29(1-2): 155-162, 1986

Essential Oils of Gardenia jasminoides J. Ellis and Gardenia jasminoides f. longicarpa Z.W. Xie and M. Okada Flowers: Chemical Characterization and Assessment of Anti-Inflammatory Effects in Alveolar Macrophage. Pharmaceutics 14(5), 2022

Induction of crocin and crocetins in callus cultures of Gardenia jasminoides Ellis. Food Biotechnology (New York) 9(1-2): 29-38, 1995

Determination of Jasminoidin in Rabbit Plasma for the Pharmacokinetic Investigation after Single Dose Oral Administration of Gardenia jasminoides Ellis and Gardenia jasminoides Ellis Coupling Coptis chinensis Franch Extracts. Chromatographia 71(11-12): 1031-1037, 2010

Effect of a red to far red light filtering plastic film on growth of gardenia (Gardenia jasminoides). Acta Horticulturae 711: 399-404, 2006

Analysis of Crocetin Derivatives from Gardenia(Gardenia jasminoides Ellis) Fruits. Nippon Shokuhin Kagaku Kogaku Kaishi 42(10): 776-783, 1995

Selective Extraction of Gardenia Yellow and Geniposide from Gardenia jasminoides by Mechanochemistry. Molecules 21(5), 2016

Attacks by Myrothecium roridum on crops of gardenia (Gardenia jasminoides) in Italy. Informatore Fitopatologico 46(10): 47-49, 1996

Histopathology of gardenia (Gardenia jasminoides veitchi), infected with three species of Meloidogyne. Nematologica 5(4): 228-230, 1960

Food Chemical Properties of Crocetin Derivatives in Gardenia(Gardenia jasminoides Ellis) Yellow Color. Nippon Shokuhin Kagaku Kogaku Kaishi 42(10): 784-789, 1995

Shoot proliferation and growth of gardenia (Gardenia jasminoides Ellis) in response to pH and sugar concentrations in vitro. PGRSA Quarterly 26(1): 1-11, 1998

Studies on the constituents of Gardenia species. III. new iridoid glycosides from the leaves of Gardenia jasminoides cv. fortuneana Hara. Chemical and Pharmaceutical Bulletin 51(12): 1417-1419, 2003

Adsorption Characteristics of Crocin in the Extract of Gardenia Fruits (Gardenia Jasminoides Ellis) on Macroporous Resins. Journal of Food Process Engineering 32(1): 35-52, 2009

Enrichment and purification of gardenia yellow from Gardenia jasminoides var. radicans Makino by column chromatography technique. Journal of Chromatography. B Analytical Technologies in the Biomedical and Life Sciences 893-894: 43-48, 2012