+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Integrated electrokinetically driven microfluidic devices with pH-mediated solid-phase extraction coupled to microchip electrophoresis for preterm birth biomarkers



Integrated electrokinetically driven microfluidic devices with pH-mediated solid-phase extraction coupled to microchip electrophoresis for preterm birth biomarkers



Electrophoresis 38(13-14): 1743-1754



Integration in microfluidics is important for achieving automation. Sample preconcentration integrated with separation in a microfluidic setup can have a substantial impact on rapid analysis of low-abundance disease biomarkers. Here, we have developed a microfluidic device that uses pH-mediated solid-phase extraction (SPE) for the enrichment and elution of preterm birth (PTB) biomarkers. Furthermore, this SPE module was integrated with microchip electrophoresis for combined enrichment and separation of multiple analytes, including a PTB peptide biomarker (P1). A reversed-phase octyl methacrylate monolith was polymerized as the SPE medium in polyethylene glycol diacrylate modified cyclic olefin copolymer microfluidic channels. Eluent for pH-mediated SPE of PTB biomarkers on the monolith was optimized using different pH values and ionic concentrations. Nearly 50-fold enrichment was observed in single channel SPE devices for a low nanomolar solution of P1, with great elution time reproducibility (<7% RSD). The monolith binding capacity was determined to be 400 pg (0.2 pmol). A mixture of a model peptide (FA) and a PTB biomarker (P1) was extracted, eluted, injected, and then separated by microchip electrophoresis in our integrated device with ∼15-fold enrichment. This device shows important progress towards an integrated electrokinetically operated platform for preconcentration and separation of biomarkers.

Please choose payment method:






(PDF emailed within 0-6 h: $19.90)

Accession: 059869092

Download citation: RISBibTeXText

PMID: 28272749

DOI: 10.1002/elps.201700054


Related references

Electrokinetically operated microfluidic devices for integrated immunoaffinity monolith extraction and electrophoretic separation of preterm birth biomarkers. Analyst 143(1): 224-231, 2017

Automated microfluidic devices integrating solid-phase extraction, fluorescent labeling, and microchip electrophoresis for preterm birth biomarker analysis. Analytical and Bioanalytical Chemistry 410(3): 933-941, 2018

3D Printed Microfluidic Devices for Microchip Electrophoresis of Preterm Birth Biomarkers. Analytical Chemistry 91(11): 7418-7425, 2019

Integrated multilayer microfluidic device with a nanoporous membrane interconnect for online coupling of solid-phase extraction to microchip electrophoresis. Lab on a Chip 7(12): 1819-1824, 2007

3D printed microfluidic devices with immunoaffinity monoliths for extraction of preterm birth biomarkers. Analytical and Bioanalytical Chemistry 411(21): 5405-5413, 2019

Design and fabrication of integrated solid-phase extraction-zone electrophoresis microchip. Journal of Chromatography. a 1111(2): 258-266, 2006

Towards an integrated microfluidic device for spaceflight clinical diagnostics Microchip-based solid-phase extraction of hydroxyl radical markers. Journal of Chromatography. a 1200(2): 198-203, 2008

Electrokinetically driven fluidic transport in integrated three-dimensional microfluidic devices incorporating gold-coated nanocapillary array membranes. Lab on a Chip 8(10): 1625-1631, 2008

Development of an integrated microfluidic solid-phase extraction and electrophoresis device. Analyst 141(5): 1660-1668, 2016

Integrated Electrokinetic Sample Fractionation and Solid Phase Extraction in Microfluidic Devices. 2012

Integrated electrokinetic sample fractionation and solid-phase extraction in microfluidic devices. Electrophoresis 33(21): 3151-3158, 2012

Poly(dimethylsiloxane)-based microchip for two-dimensional solid-phase extraction-capillary electrophoresis with an integrated electrospray emitter tip. Analytical Chemistry 77(16): 5356-5363, 2005

Determination of haloacetic acids in water using solid-phase extraction/microchip capillary electrophoresis with capacitively coupled contactless conductivity detection. Electrophoresis 31(15): 2602-2607, 2010

Microchip electrophoresis separation of a panel of preterm birth biomarkers. Electrophoresis 39(18): 2300-2307, 2018

Integrated microfluidic device for solid-phase extraction coupled to micellar electrokinetic chromatography separation. Analytical Chemistry 77(20): 6664-6670, 2005