+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Lactobionic acid enhances the synergistic effect of nisin and thymol against Listeria monocytogenes Scott A in tryptic soy broth and milk



Lactobionic acid enhances the synergistic effect of nisin and thymol against Listeria monocytogenes Scott A in tryptic soy broth and milk



International Journal of Food Microbiology 260: 36-41



Listeria monocytogenes is a Gram-positive opportunistic human pathogen and it remains a significant cause of foodborne illnesses. A variety of natural and synthetic compounds have been studied to inhibit the growth of L. monocytogenes in foods. Antimicrobial combinations with synergistic antilisterial properties can reduce the dose of each antimicrobial, which can be further enhanced by chelating compounds. Therefore, the objective of this study was to determine antilisterial properties of binary or ternary combinations of lactobionic acid (LBA), nisin, and thymol in tryptic soy broth (TSB), 2% reduced-fat milk, and whole milk. The results showed that the minimum inhibitory concentration (MIC) of nisin, thymol and LBA was 125IU/mL, 0.25mg/mL, and 10mg/mL, respectively. The ternary combination was the most effective in reducing MICs of antimicrobials, with the MIC of nisin, thymol, and LBA being 31.25IU/mL, 0.0625mg/mL, and 1.25mg/mL, respectively. In TSB with 0.6% yeast extract, L. monocytogenes grew in individual or binary antimicrobial treatments of 31.25IU/mL nisin, 0.0625mg/mL thymol, and 1.25mg/mL LBA within 24h at 32°C, while it was completely inhibited by the ternary combination. In 2% reduced-fat milk at 21°C, the ternary combination of nisin, thymol, and LBA at respective concentrations of 250IU/mL, 2mg/mL, and 10mg/mL completely inhibited the bacterium to below the detection limit in 72h while >2log (CFU/mL) bacteria was still detected in all the binary combinations after 120h. In whole milk, the combination of 500IU/mL nisin, 2mg/mL thymol, and 10mg/mL LBA reduced bacteria to around 2log (CFU/mL) in 4h at 21°C, and no bacterial recovery was observed after 5 d. This study suggested the potential of the ternary combination of nisin, thymol and LBA for food preservation.

Please choose payment method:






(PDF emailed within 0-6 h: $19.90)

Accession: 059906315

Download citation: RISBibTeXText

PMID: 28843122

DOI: 10.1016/j.ijfoodmicro.2017.08.013


Related references

Synergistic effects of nisin and thymol on antimicrobial activities in Listeria monocytogenes and Bacillus subtilis. FEMS microbiology letters 2000 183(1): 191-195, 2000

Intracellular pH and Survival of Listeria monocytogenes Scott A in Tryptic Soy Broth Containing Acetic, Lactic, Citric, and Hydrochloric Acids. Journal of Food Protection 54(1): 15-19, 1991

Synergistic effect of nisin and the lactoperoxidase system on Listeria monocytogenes in skim milk. International journal of food microbiology 40(1-2): 35-42, 1998

Synergistic effect of nisin and garlic shoot juice against Listeria monocytogenes in milk. Food Chemistry 110(2): 375-382, 2008

Synergistic effect of nisin and cone essential oil of Metasequoia glyptostroboides Miki ex Hu against Listeria monocytogenes in milk samples. Food and Chemical Toxicology 49(1): 109-114, 2011

Suppression of Listeria monocytogenes Scott A in Fluid Milk by Free and Liposome-Entrapped Nisin. Probiotics and Antimicrobial Proteins 1(2): 152-158, 2009

Survival of Listeria monocytogenes Scott A on vacuum-packaged raw beef treated with polylactic acid, lactic acid, and nisin. Journal of Food Protection 63(1): 131-136, 2000

Induced acid-tolerance response confers limited nisin resistance on Listeria monocytogenes Scott A. Journal of Food Protection 59(9): 1003-1006, 1996

Use of bacteriocinogenic lactic acid bacteria to inhibit spontaneous nisin-resistant mutants of Listeria monocytogenes Scott A. Journal of Applied Microbiology 85(4): 657-663, 1998

Effect of nisin on growth boundaries of Listeria monocytogenes Scott A, at various temperatures, pH and water activities. Food Microbiology 23(8): 779-784, 2006

The combined effect of nisin, leucocin F10, pH, NaCl and EDTA on the survival of Listeria monocytogenes in broth. International journal of food microbiology 40(1-2): 65-75, 1998

Combined antimicrobial effect of nisin and a listeriophage against Listeria monocytogenes in broth but not in buffer or on raw beef. International journal of food microbiology 73(1): 71-81, 2002

Mechanism of synergistic inhibition of Listeria monocytogenes growth by lactic acid, monolaurin, and nisin. Applied and Environmental Microbiology 74(23): 7126-7129, 2008

Protein profile changes in acid adapted Listeria monocytogenes exhibiting cross-protection against an activated lactoperoxidase system in tryptic soy broth. Journal of food safety 20(1): 27-42, 2000

The synergistic effect of nisin and lactoferrin on the inhibition of Listeria monocytogenes and Escherichia coli O157:H7. Letters in Applied Microbiology 44(3): 255-261, 2007