+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Non-Invasive Liver Ablation Using Histotripsy: Preclinical Safety Study in an In Vivo Porcine Model



Non-Invasive Liver Ablation Using Histotripsy: Preclinical Safety Study in an In Vivo Porcine Model



Ultrasound in Medicine and Biology 43(6): 1237-1251



This study investigates the safety profile for use of histotripsy, a non-invasive ultrasonic ablation method currently being developed for the treatment of liver cancer, for liver ablation in an in vivo porcine model. Histotripsy treatments were applied to the liver and hepatic veins of 22 porcine subjects, with half of the subjects receiving systemic heparinization. Vital signs (heart rate, blood pressure, temperature, electrocardiogram and SpO2) were monitored throughout the procedure and for 1 h post-treatment. Blood was drawn at six points during the experiment to analyze blood gases, liver function and free hemoglobin levels. All treatments were guided and monitored by real-time ultrasound imaging. After treatment, the tissue was harvested for histological analysis. Results indicated that histotripsy generated well-defined lesions inside the liver and around the treated hepatic veins of all subjects in both treatment groups. Vital signs and blood analysis revealed that animals responded well to histotripsy, with all animals surviving the treatment. One animal in the non-heparinized group had a transient increase in pH and decreases in blood pressure, heart rate and PCO2 during the 15-min vessel treatment, with these changes returning to baseline levels soon after the treatment. Overall, the results indicate that histotripsy can safely be performed on the liver without the need for systemic heparinization, even in regions containing large hepatic vessels, supporting its future use for the treatment of liver cancer.

Please choose payment method:






(PDF emailed within 0-6 h: $19.90)

Accession: 060024025

Download citation: RISBibTeXText

PMID: 28318889

DOI: 10.1016/j.ultrasmedbio.2017.01.016


Related references

Image-guided non-invasive ultrasound liver ablation using histotripsy: feasibility study in an in vivo porcine model. Ultrasound in Medicine and Biology 39(8): 1398-1409, 2013

Non-Invasive Ultrasound Liver Ablation Using Histotripsy: Chronic Study in an In Vivo Rodent Model. Ultrasound in Medicine and Biology 42(8): 1890-1902, 2016

Histotripsy: minimally invasive technology for prostatic tissue ablation in an in vivo canine model. Urology 72(3): 682-686, 2008

Multipolar RFA of the liver: Influence of intrahepatic vessels on ablation zones and appropriateness of CECT in detecting ablation dimensions - Results of an in-vivo porcine liver model. Clinical Hemorheology and Microcirculation 70(4): 467-476, 2018

Pd19-11 Pilot Assessment Of Transcutaneous Boiling Histotripsy Ablation Of The Kidney In The Porcine Model. The Journal of Urology 195(4): e449-e450, 2016

Histotripsy for Non-Invasive Ablation of Hepatocellular Carcinoma (HCC) Tumor in a Subcutaneous Xenograft Murine Model. Conference Proceedings 2018: 6064-6067, 2018

Developmental impact and lesion maturation of histotripsy-mediated non-invasive tissue ablation in a fetal sheep model. Ultrasound in Medicine and Biology 39(6): 1047-1055, 2013

Microwave ablation with loop antenna: in vivo porcine liver model. Radiology 231(1): 143-149, 2004

Preclinical evaluation of an MR-compatible microwave ablation system and comparison with a standard microwave ablation system in an ex vivo bovine liver model. International Journal of Hyperthermia 33(6): 617-623, 2017

Optimization of a novel microwave tumor ablation system in an in vivo porcine liver model. Gastroenterology 123(1 Suppl.): 14, 2002

Effect of Hepatic Perfusion on Microwave Ablation Zones in an Ex Vivo Porcine Liver Model. Journal of Vascular and Interventional Radiology 28(5): 732-739, 2017

Potential mechanisms of the heat-sink effect during microwave ablation of an in-vivo porcine liver model. Journal of Vascular and Interventional Radiology 26(2): S117-S118, 2015

Perivascular extension of microwave ablation zone: demonstrated using an ex vivo porcine perfusion liver model<sup/>. International Journal of Hyperthermia 34(7): 1114-1120, 2018

Evaluation of 3-D computer-simulated irradiation model for interstitial tumor ablation in porcine liver in vivo. Gastroenterology 120(5 Suppl. 1): A 346, 2001

Microwave ablation with a single small-gauge triaxial antenna: in vivo porcine liver model. Radiology 242(2): 435-440, 2007