EurekaMag
+ Most Popular
Cunninghamia lanceolata plantations in China
Mammalian lairs in paleo ecological studies and palynology
Studies on technological possibilities in utilization of anhydrous milk fat for production of recombined butter-like products
Should right-sided fibroelastomas be operated upon?
Large esophageal lipoma
Apoptosis in the mammalian thymus during normal histogenesis and under various in vitro and in vivo experimental conditions
Poissons characoides nouveaux ou non signales de l'Ilha do Bananal, Bresil
Desensitizing efficacy of Colgate Sensitive Maximum Strength and Fresh Mint Sensodyne dentifrices
Administration of fluid by subcutaneous infusion: revival of a forgotten method
Tundra mosquito control - an impossible dream?
Schizophrenia for primary care providers: how to contribute to the care of a vulnerable patient population
Geochemical pattern analysis; method of describing the Southeastern limestone regional aquifer system
Incidence of low birth weights in a hospital of Mexico City
Tabanidae
Graded management intensity of grassland systems for enhancing floristic diversity
Microbiology and biochemistry of cheese and fermented milk
The ember tetra: a new pygmy characid tetra from the Rio das Mortes, Brazil, Hyphessobrycon amandae sp. n. (Pisces, Characoidei)
Risk factors of contrast-induced nephropathy in patients after coronary artery intervention
Renovation of onsite domestic wastewater in a poorly drained soil
Observations of the propagation velocity and formation mechanism of burst fractures caused by gunshot
Systolic blood pressure in a population of infants in the first year of life: the Brompton study
Haematological studies in rats fed with metanil yellow
Studies on pasteurellosis. I. A new species of Pasteurella encountered in chronic fowl cholera
Dormancy breaking and germination of Acacia salicina Lindl. seeds
therapy of lupus nephritis. a two-year prospective study

Pyridoxal 5'-phosphate, pyridoxal, and 4-pyridoxic acid in the paired serum and cerebrospinal fluid of children


Pyridoxal 5'-phosphate, pyridoxal, and 4-pyridoxic acid in the paired serum and cerebrospinal fluid of children



Clinica Chimica Acta; International Journal of Clinical Chemistry 472: 118-122



ISSN/ISBN: 0009-8981

PMID: 28778380

DOI: 10.1016/j.cca.2017.07.032

We quantified pyridoxal 5'-phosphate (PLP), pyridoxal (PL), and 4-pyridoxic acid (PA) in paired serum and cerebrospinal fluid (CSF) samples from children and investigated the effect of age on the concentrations and CSF-to-serum ratios of these vitamers. Serum and CSF samples prospectively collected from 49 pediatric patients were analyzed. PLP, PL, and PA were measured using high-performance liquid chromatography with fluorescence detection, using pre-column derivatization by semicarbazide. Effects of age on these vitamers, the PLP-to-PL ratio, CSF-to-serum PLP ratio, and CSF-to-serum PL ratio were evaluated using correlation analysis. The PLP, PL, and PA concentrations in the serum and CSF were higher at younger ages, except for CSF PA concentrations that were mostly below the limit of detection (<1.2nmol/l). The PLP-to-PL ratios in the serum and CSF correlated positively with age. The CSF-to-serum PLP ratio and CSF-to-serum PL ratio were independent of age. Age-related changes in PLP, PL, and PA in serum and in CSF from pediatric patients and CSF-to-serum ratios of PLP and PL demonstrated in this study will provide valuable information for evaluating PLP supply to the central nervous system from the peripheral blood.

Please choose payment method:






(PDF emailed within 0-6 h: $19.90)

Accession: 060158295

Download citation: RISBibTeXText

Related references

Measurement of pyridoxal 5'-phosphate, pyridoxal, and 4-pyridoxic acid in the cerebrospinal fluid of children. Clinica Chimica Acta; International Journal of Clinical Chemistry 466: 1-5, 2017

Developmental course of pyridoxal phosphate pyridoxal kinase pyridoxine phosphate oxidase pyridoxal phosphate phosphatase and 4 pyridoxic acid in the central nervous system. Federation Proceedings 29(2): 479, 1970

Simultaneous and direct determination of pyridoxal, pyridoxal-5'-phosphate, and pyridoxic acid in serum by derivative synchronous fluorescence spectroscopy. Analytical Biochemistry 157(2): 212-220, 1986

Serum B 6 vitamers (pyridoxal 5'-phosphate, pyridoxal, and 4-pyridoxic acid) and pancreatic cancer risk: two nested case-control studies in Asian populations. Cancer Causes and Control: Ccc 27(12): 1447-1456, 2016

Assay of pyridoxal-5'-phosphate, pyridoxal and pyridoxic acid in biological material. International Journal for Vitamin and Nutrition Research. Internationale Zeitschrift für Vitamin- und Ernahrungsforschung. Journal International de Vitaminologie et de Nutrition 59(4): 338-343, 1989

Formation of 4-pyridoxic acid and 4-pyridoxic acid lactone from pyridoxal-5'-phosphate in liver homogenate. Heterocyclic compounds in metabolism. 5. Biochem. Ztschr 329: 451-457, 1958

Determination of pyridoxal 5'-phosphate in human serum by reversed phase high performance liquid chromatography combined with spectrofluorimetric detection of 4-pyridoxic acid 5'-phosphate as a derivative. Analyst 114(10): 1225-1228, 1989

Serum concentrations of the pyridoxal and pyridoxal-5'-phosphate in children during sustained-release theophylline therapy. Arerugi 45(10): 1098-1105, 1996

Changes in serum concentrations of folates, pyridoxal, pyridoxal-5-phosphate and vitamin B12 during lactation of dairy cows fed dietary supplements of folic acid. Canadian journal of animal science 79(1): 107-113, 1999

Vitamin B 6 depletion in man: blood vitamin B 6, plasma pyridoxal-phosphate, serum cholesterol, serum transminases and urinary vitamin B 6 and 4-pyridoxic acid. Journal of Nutrition 89(1): 19-23, 1966

Clinical analysis of vitamin B(6): determination of pyridoxal 5'-phosphate and 4-pyridoxic acid in human serum by reversed-phase high-performance liquid chromatography with chlorite postcolumn derivatization. Analytical Biochemistry 333(2): 336-344, 2004

Role of pyridoxal 5'-phosphate in glycogen phosphorylase. I. Synthesis of 3'-O-methylpyridoxal 5'-phosphate N-oxide and pyridoxal 5'-phosphate monomethyl ester and the conversion of the N-oxide to pyridoxal 5'-phosphate by apophosphorylase b from rabbit skeletal muscle. Biochemistry 11(11): 2125-2136, 1972

A simple, sensitive and reproducible assay for pyridoxal 5'-phosphate and 4-pyridoxic acid in human plasma. Clinica Chimica Acta; International Journal of Clinical Chemistry 280(1-2): 101-111, 1999

Role of pyridoxal 5'-phosphate in glycogen phosphorylase. II. Mode of binding of pyridoxal 5'-phosphate and analogs of pyridoxal 5'-phosphate to apophosphorylase b and the aggregation state of the reconstituted phosphorylase proteins. Biochemistry 11(11): 2136-2145, 1972

Conformational alteration in serum albumin as a carrier for pyridoxal phosphate: a distinction from pyridoxal phosphate-dependent glutamate decarboxylase. Archives of Biochemistry and Biophysics 364(2): 195-202, 1999