Reactivity of bilirubin photoisomers on the measurement of direct bilirubin using vanadic acid method
Okada, H.; Itoh, S.; Kawamoto, S.; Ozaki, M.; Kusaka, T.
Annals of Clinical Biochemistry 55(2): 296-298
ISSN/ISBN: 1758-1001 PMID: 28485173 DOI: 10.1177/0004563217709844
Objective Investigation of the reactivity of fractions of bilirubin photoisomers with the vanadic acid oxidation method. Methods Bilirubin photoisomers were prepared by irradiating a bilirubin/human serum albumin solution with blue light emitting diode. Direct bilirubin and bilirubin fractions were measured using the vanadic acid oxidation method and high-performance liquid chromatography in the sample before and after irradiation. Results Direct bilirubin was increased in the solution containing bilirubin photoisomers. ( EE)-/( EZ) -cyclobilirubin-IXα and ( ZE)-/( EZ)-bilirubin-IXα completely disappeared after the addition of vanadic acid. Conclusion Bilirubin photoisomers reacted as direct bilirubin in the vanadic acid oxidation method.