+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Silica nanoparticles induce NLRP3 inflammasome activation in human primary immune cells

Silica nanoparticles induce NLRP3 inflammasome activation in human primary immune cells

Innate Immunity 23(8): 697-708

In recent years, the potential use of silica nanoparticles (SiNPs) among different biomedical fields has grown. A deep understanding of the physicochemical properties of nanoparticles (NPs) and their regulation of specific biological responses is crucial for the successful application of NPs. Exposure to NP physicochemical properties (size, shape, porosity, etc.) could result in deleterious effects on cellular functions, including a pro-inflammatory response mediated via activation of the NLRP3 inflammasome. The aim of this study was to evaluate the potential in vitro immunomodulatory effect of 12-nm and 200-nm SiNPs on the expression of pro-inflammatory cytokines and NLRP3 inflammasome components in human primary neutrophils and PBMCs. This study demonstrates that regardless of the size of the nanoparticles, SiNPs induce the production of pro-inflammatory cytokines in a dose-dependent manner. Induced IL-1β production after exposure to SiNPs suggests the involvement of NLRP3 inflammasome components participation in this process. In conclusion, SiNPs induce the production of pro-inflammatory cytokines in a dose-dependent manner. Furthermore, our data suggest that the production and release of IL-1β possibly occurs through the formation of the NLRP3 inflammasome.

Please choose payment method:

(PDF emailed within 0-6 h: $19.90)

Accession: 060249818

Download citation: RISBibTeXText

PMID: 29113588

DOI: 10.1177/1753425917738331

Related references

Silica induces NLRP3 inflammasome activation in human lung epithelial cells. Particle and Fibre Toxicology 10: 3, 2013

Polymeric Nanoparticles Induce NLRP3 Inflammasome Activation and Promote Breast Cancer Metastasis. Macromolecular Bioscience 17(12):, 2017

Urate crystals induce NLRP3 inflammasome-dependent IL-1β secretion and proliferation in isolated primary human T-cells. Hippokratia 19(1): 41-46, 2015

Mesoporous silica nanoparticles induced hepatotoxicity via NLRP3 inflammasome activation and caspase-1-dependent pyroptosis. Nanoscale 10(19): 9141-9152, 2018

Hyperosmotic Stress-Induced TRPM2 Channel Activation Stimulates NLRP3 Inflammasome Activity in Primary Human Corneal Epithelial Cells. Investigative Ophthalmology and Visual Science 59(8): 3259-3268, 2018

Assessment of inflammasome activation in primary human immune cells. Methods in Molecular Biology 1040: 29-39, 2013

Reactive oxygen species trigger NF-κB-mediated NLRP3 inflammasome activation induced by zinc oxide nanoparticles in A549 cells. Toxicology and Industrial Health 33(10): 737-745, 2017

Evidence of NLRP3-inflammasome activation in rheumatoid arthritis (RA); genetic variants within the NLRP3-inflammasome complex in relation to susceptibility to RA and response to anti-TNF treatment. Annals of the Rheumatic Diseases 73(6): 1202-1210, 2014

Silica-induced NLRP3 inflammasome activation in vitro and in rat lungs. Particle and Fibre Toxicology 11: 58, 2014

Precipitation of Soluble Uric Acid Is Necessary for In Vitro Activation of the NLRP3 Inflammasome in Primary Human Monocytes. Journal of Rheumatology 46(9): 1141-1150, 2019

Assessing NLRP3 Inflammasome Activation by Nanoparticles. Methods in Molecular Biology 1682: 135-147, 2018

The NLRP3 inflammasome activation in human or mouse cells, sensitivity causes puzzle. Protein and Cell 4(8): 565-568, 2013

Induction of NLRP3 Inflammasome Activation by Heme in Human Endothelial Cells. Oxidative Medicine and Cellular Longevity 2018: 4310816, 2018

K+ efflux agonists induce NLRP3 inflammasome activation independently of Ca2+ signaling. Journal of Immunology 194(8): 3937-3952, 2015

Activation of the inflammasome by amorphous silica and TiO2 nanoparticles in murine dendritic cells. Nanotoxicology 5(3): 326-340, 2011