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Solutions to decrease a systematic error related to AAPH addition in the fluorescence-based ORAC assay



Solutions to decrease a systematic error related to AAPH addition in the fluorescence-based ORAC assay



Analytical Biochemistry 519: 27-29



Oxygen radical absorbance capacity (ORAC) assay in 96-well multi-detection plate readers is a rapid method to determine total antioxidant capacity (TAC) in biological samples. A disadvantage of this method is that the antioxidant inhibition reaction does not start in all of the 96 wells at the same time due to technical limitations when dispensing the free radical-generating azo initiator 2,2'-azobis (2-methyl-propanimidamide) dihydrochloride (AAPH). The time delay between wells yields a systematic error that causes statistically significant differences in TAC determination of antioxidant solutions depending on their plate position. We propose two alternative solutions to avoid this AAPH-dependent error in ORAC assays.

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Accession: 060263650

Download citation: RISBibTeXText

PMID: 27965064

DOI: 10.1016/j.ab.2016.12.009


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