Studies on oxidative phosphorylation: XIII. Relationship of coupling factors to stimulation by oligomycin of energy-linked reactions
Ziegelhoeffer A.; De Jong J.W.; Ferrari R.; Turi Nagy L.
Archives of Biochemistry and Biophysics 117(3): 594-598
ISSN/ISBN: 0003-9861 DOI: 10.1016/0003-9861(66)90100-7
The stimulation of energy-linked reactions by low levels of oligomycin varies greatly with the type of submitochondrial particle. Particles made by disruption of combined heavy and light mitochondria by exposure to sonic oscillation and depleted by urea treatment showed no stimulation over a wide range of oligomycin concentration. However, the mitochondrial coupling factor described previously stimulated energy-linked reactions in these particles. Similar particles from heavy mitochondria gave some stimulation with low levels of oligomycin. High stimulation was obtained with alkali-treated particles derived from heavy mitochondria. The magnitude of stimulation appeared to be related to the concentration of endogenous coupling factors.