Section 62
Chapter 61,531

Bacteriophage lambda-E. coliK12 vector-host system for gene cloning and expression under lactose promoter control

Christine Pourcel; Christian Marchal; Anne Louise; Alexandre Fritsch; Pierre Tiollais

Molecular Genetics and Genomics 170(2): 161-169


ISSN/ISBN: 1617-4615
DOI: 10.1007/bf00337792
Accession: 061530274

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Bacteriophage lambda vectors, derived from lambda plac5 were constructed. Their genomes have only one EcoRI restriction site, located near the end of the beta-galactosidase gene. Recombinants, constructed in vitro, having a DNA fragment inserted in the EcoRI site, are lac- and can be easily recognized. Expression of such foreign genes is then under the control of the lac promoter.

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