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Bacteriophage lambda-E. coliK12 vector-host system for gene cloning and expression under lactose promoter control

Christine Pourcel; Christian Marchal; Anne Louise; Alexandre Fritsch; Pierre Tiollais

Molecular Genetics and Genomics 170(2): 161-169

1979

ISSN/ISBN: 1617-4615
DOI: 10.1007/bf00337792
Accession: 061530274
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Bacteriophage lambda vectors, derived from lambda plac5 were constructed. Their genomes have only one EcoRI restriction site, located near the end of the beta-galactosidase gene. Recombinants, constructed in vitro, having a DNA fragment inserted in the EcoRI site, are lac- and can be easily recognized. Expression of such foreign genes is then under the control of the lac promoter.

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Related References

Charnay, P.; Louise, A.; Fritsch, A.; Perrin, D.; Tiollais, P. 1979: Bacteriophage lambda-E. coli K12 vector-host system for gene cloning and expression under lactose promoter control. II. DNA fragment insertion at the vicinity of the lac UV5 promoter Molecular and General Genetics: Mgg 170(2): 171-178
Pourcel, C.; Marchal, C.; Louise, A.; Fritsch, A.; Tiollais, P. 1979: Bacteriophage lambda-E. coli K12 vector-host system for gene cloning and expression under lactose promoter control: I. DNA fragment insertion at the lacZ EcoRi restriction site Molecular and General Genetics: Mgg 170(2): 161-169
Pourcel, C.; Marchal, C.; Louise, A.; Fritsch, A.; Tiollais, P. 1979: Bacterio phage lambda escherichia coli k 12 vector host system for gene cloning and expression under lactose promoter control part 1 dna fragment insertion at the lacz eco r i restriction site Molecular and General Genetics 170(2): 161-170
Kuypers, B.; Reiser, W.; Klein, A. 1980: Cloning of the replication gene O of E. coli bacteriophage lambda and its expression under the control of the lac promoter Gene 10(3): 195-203
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Cohen, S.N.; Chang, A.C. 1971: Genetic expression in bacteriophage lambda. IV. Effects of P2 prophage on lambda inhibition of host synthesis and lambda gene expression Virology 46(2): 397-406
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de Vries, G.E.; Raymond, C.K.; Ludwig, R.A. 1984: Extension of bacteriophage lambda host range: selection, cloning, and characterization of a constitutive lambda receptor gene Proceedings of the National Academy of Sciences of the United States of America 81(19): 6080-6084
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