A relationship between plasmid structure, structural lability, and sensitivity to site-specific endonucleases inNeisseria gonorrhoeae
John, K. Davies; Staffan Normark
Molecular Genetics and Genomics 177(2): 251-260
Nearly all gonococcal strains carry a small "phenotypically cryptic" plasmid of approximately 4,200 basepairs. A detailed physical map of this plasmid has been constructed, revealing the presence of numerous putative inverted repeats. These studies also revealed the presence on the plasmid of recognition sequences for several site-specific endonucleases (particularly HpaII, MspI and AluI) that are particularly resistant to cleavage, and confirmed previous reports of structural lability.