Electron transport reactions in respiratory particles of hydrogenase-induced Anacystis nidulans
Günter A. Peschek
Archives of Microbiology 125(1-2): 123-131
ISSN/ISBN: 0302-8933 DOI: 10.1007/bf00403208
Respiratory particles from hydrogen-grown Anacystis nidulans were found to oxidize H2, NADPH, NADH, succinate and ascorbate plus N,N,N′,N′-tetramethyl-p-phenylenediamine at rates corresponding to 28, 15, 6, 2.5, and 70 nmol O2 taken up x mg protein−1xmin−1, respectively. The particles were isolated by brief sonication of lysozyme-pretreated cells. Respiratory activities were studied in terms of both substrate oxidation and O2 uptake.