Plasmid-mediated transfer of nitrogen-fixing capability to bacteria from the rhizosphere of grasses
Kleeberger, A.; Klingmuller, W.
Molecular Genetics and Genomics 180(3): 621-627
1980
ISSN/ISBN: 1617-4615
DOI: 10.1007/bf00268070
Accession: 061591911
Cells of a non-nitrogen fixing, drug-sensitive Enterobacter cloacae strain, isolated from the rhizosphere of Festuca heterophylla, were mated to Escherichia coli cells harboring plasmid pRD1. This plasmid carries the nitrogen-fixation (nif+) genes and 3 markers of drug resistance. After mating, triple-resistant Enterobacter transferants could be selected. These were screened for plasmids, acetylene reduction and stability of the transferred markers. Transferants contained plasmid pRD1.