Section 62
Chapter 61,597

Simultaneous determination of theophylline and its major metabolites in urine by reversed-phase ion-pair high-performance liquid chromatography

Keith, T. Muir; Jan, H.G. Jonkman; Dan-Shya Tang; Michael Kunitani; Sidney Riegelman

Journal of Chromatography B: Biomedical Sciences and Applications 221(1): 85-95


DOI: 10.1016/s0378-4347(00)81010-5
Accession: 061596967

A new, highly selective high-performance liquid-chromatographic (HPLC) assay for the bronchodilator theophylline and its major metabolites in human urine is described. The method utilizes an ion-pair extraction followed by separation and quantitation by reversed-phase ion-pair gradient-elution HPLC.

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