Home
  >  
Section 63
  >  
Chapter 62,062

Purification and characterization of a neutral serine protease from non-sulfur purple photosynthetic bacterium

Moon-Sik Hyun; Shin-ichi Okuda; Kazuo Izaki

Current Microbiology 18(6): 379-383

1989

ISSN/ISBN: 0343-8651
DOI: 10.1007/bf01571132
Accession: 062061282
Download citation:  
Text
  |  
BibTeX
  |  
RIS
A neutral serine protease was purified as a homogeneous protein from the culture broth of photosynthetic bacterium T-20 by sequential chromatographies on columns of DEAE-cellulose. Toyopearl HW 55F, hydroxyapatite, and CM-cellulose. The molecular weight was estimated to be approximately 44,000 by SDS-PAGE, while the value of approximately 80,000 was obtained when the Hedrick-Smith method was used; this suggested that the enzyme consists of two identical subunits. The isoelectric point was determined to be 6.3 by isoelectric focusing. The enzyme had a pH optimum at 7.8. Maximal enzyme activity was detected at 50.degree.C, and the activity was stable up to 50.degree.C for 5 min at pH 7.0-7.2.

Full Text Article emailed within 0-6 h: $19.90




Related References

Lerluck Buranakarl; Kazutoshi Ito; Kazuo Izaki; Hajime Takahashi 1988: Purification and characterization of a raw starch-digestive amylase from non-sulfur purple photosynthetic bacterium Enzyme and Microbial Technology 10(3): 173-179
Inui, M.; Momma, K.; Matoba, R.; Ikuta, M.; Yamagata, H.; Yukawa, H. 1995: Characterization of alcohol-assimilating photosynthetic purple non-sulfur bacteria and cloning of molecular chaperones from a purple non-sulfur bacterium Energy Convers. Manage 36(6-9): 767-770
Stadtwald, R.; Favinger, J.; Gest, H. 1989: Characterization of rhodospirillum centenum new species a novel non sulfur purple photosynthetic bacterium Abstracts of the Annual Meeting of the American Society for Microbiology 89: 226
Garcia Castillo, M.C.; Finnegan, M.G.; Conover, R.C.; Knaff, D.B.; Johnson, M.K. 1994: Spectroscopic characterization of flavocytochrome c-552 from the photosynthetic purple sulfur bacterium Chromatium vinosum Biochimica et Biophysica Acta 1184(2-3): 273-278
Kerfeld, C.; Thornber, P.; Yeates, T. 1992: Biochemical and structural characterization of the photosynthetic apparatus of a purple-sulfur bacterium Chromatium purpuratum Murata, N Research in photosynthesis, Vol 1; IXth International Congress on Photosynthesis : 49-52
Hu, Q.; Brunisholz, R.A.; Frank, G.; Zuber, H. 1996: The antenna complexes of the purple non-sulfur photosynthetic bacterium Rhodocyclus tenuis. Structural and spectral characterization European Journal of Biochemistry 238(2): 381-390
Wei, H.; Okunishi, S.; Yoshikawa, T.; Kamei, Y.; Maeda, H. 2016: Isolation and Characterization of a Purple Non-Sulfur Photosynthetic Bacterium Rhodopseudomonas faecalis Strain a from Swine Sewage Wastewater Biocontrol Science 21(1): 29-36
Algueró, M.; Dahl, C.; Trüper, H.G. 1988: Partial purification and characterization of ADP sulfurylase from the purple sulfur bacterium Thiocapsa roseopersicina Microbiologia 4(3): 149-160
Oda, K.; Tanskul, S.; Oyama, H.; Noparatnaraporn, N. 2004: Purification and characterization of alkaline serine proteinase from photosynthetic bacterium, Rubrivivax gelatinosus KDDS1 Bioscience Biotechnology and Biochemistry 68(3): 650-655
Suzuki, H.; Hirano, Y.; Kimura, Y.; Takaichi, S.; Kobayashi, M.; Miki, K.; Wang, Z.-Y. 2007: Purification, characterization and crystallization of the core complex from thermophilic purple sulfur bacterium Thermochromatium tepidum Biochimica et Biophysica Acta 1767(8): 1057-1063
Garcia, D.; Parot, P.; Vermeglio, A. 1987: Purification and characterization of the photochemical reaction center of the thermophilic purple sulfur bacterium chromatium tepidum Biochimica et Biophysica Acta 894(3): 379-385
Kovács, A.T.; Rákhely, Gábor.; Kovács, Kél.L. 2003: Genes involved in the biosynthesis of photosynthetic pigments in the purple sulfur photosynthetic bacterium Thiocapsa roseopersicina Applied and Environmental Microbiology 69(6): 3093-3102
Wang, B.; Wu, W.; Liu, X. 2007: Purification and characterization of a neutral serine protease with nematicidal activity from Hirsutella rhossiliensis Mycopathologia 163(3): 169-176
Wang, Q.-F.; Miao, J.-L.; Hou, Y.-H.; Ding, Y.; Wang, G.-D.; Li, G.-Y. 2005: Purification and characterization of an extracellular cold-active serine protease from the psychrophilic bacterium Colwellia sp. NJ341 Biotechnology Letters 27(16): 1195-1198
Ghafoori, H.; Askari, M.; Sarikhan, S. 2016: Purification and characterization of an extracellular haloalkaline serine protease from the moderately halophilic bacterium, Bacillus iranensis (X5B) Extremophiles: Life Under Extreme Conditions 20(2): 115-123
Cammack, R.; Kovacs, K.L.; Mccracken, J.; Peisach, J. 1989: Spectroscopic characterization of the nickel and iron-sulphur clusters of hydrogenase from the purple photosynthetic bacterium Thiocapsa roseopersicina. 2. Electron spin-echo spectroscopy European Journal of Biochemistry 182(2): 363-366
Zeng, R.; Zhang, R.; Zhao, J.; Lin, N. 2003: Cold-active serine alkaline protease from the psychrophilic bacterium Pseudomonas strain DY-A: enzyme purification and characterization Extremophiles: Life Under Extreme Conditions 7(4): 335-337
Tedro, S.M.; Meyer, T.E.; Kamen, M.D. 1976: Primary structure of a high potential iron-sulfur protein from the purple non-sulfur photosynthetic bacterium Rhodopseudomonas gelatinosa Journal of Biological Chemistry 251(1): 129-136
Tedro, S.M.; Meyer, T.E.; Kamen, M.D. 1976: Primary structure of a high potential iron-sulfur protein from the purple non-sulfur photosynthetic bacterium Rhodopseudomonas gelatinosa Journal of Biological Chemistry 251(1): 129-136
Kerfeld, C.A.; Yeates, T.O.; Thornber, J.P. 1994: Purification and characterization of the peripheral antenna of the purple-sulfur bacterium Chromatium purpuratum: evidence of an unusual pigment-protein composition Biochemistry 33(8): 2178-2184