Section 63
Chapter 62,220

Automated high-performance liquid chromatographic method for the simultaneous determination of cefotiam and Δ3-cefotiam in human plasma using column switching

Kenji Yamashita; Michio Motohashi; Takatsuka Yashiki

Journal of Chromatography. B, Biomedical Sciences and Applications 577(1): 174-179


ISSN/ISBN: 1387-2273
DOI: 10.1016/0378-4347(92)80616-x
Accession: 062219049

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An automated high-performance liquid chromatographic method using column switching was established for the simultaneous determination of cefotiam (I) and 3-cefotiam (II) in human plasma after oral administration of cefotiam hexetil dihydrochloride. The method allowed the determination of analytes in plasma by the direct injection of diluted specimen with phosphate buffer. The analytes were enriched onto the C18 short pretreatment column by 0.05 M phosphate buffer (p H 7.7), while proteins and endogenous hydrophilic substances in plasma were washed off to waste. The enriched analytes were then back-flushed onto the analytical C18 column, separated by a mixture of 0.05 M phosphate buffer (p H 7.7)- acetonitrile (88:12, v/v) and detected by the ultraviolet absorbance at 254 nm. Recoveries from spiked plasma were quantitative, and the coefficients of variation were below 4%. The lower detection limits in plasma were 10 ng/ml for both I and II. Concentrations of I and Ii in plasma determined by the present method were in good agreement with those obtained by the conventional deproteinization method.

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