Section 63
Chapter 62,336

Site-directed mutagenesis of the cGMP phosphodiesterase subunit from bovine rod outer segments: Role of separate amino acid residues in the interaction with catalytic subunits and transducin a subunit

Valery, M. Lipkin; Vladimir, A. Bondarenko; Vasily, E. Zagranichny; Lyudmila, N. Dobrynina; Khakim, G. Muradov; Mikhael Yu. Natochin

Biochimica et Biophysica Acta - Molecular Cell Research 1176(3): 250-256


ISSN/ISBN: 0167-4889
DOI: 10.1016/0167-4889(93)90052-q
Accession: 062335863

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The recombinant and 21 mutant phosphodiesterase (PDE) γ subunit (PDEγ) genes were expressed by sequential transcription and translation in vitro. Inhibitory properties of these mutants and their interactions with Pde catalytic and transducin α subunits were studied. The interaction of the Pde γ subunit with the catalytic ones proceeds in two steps - primary binding and inhibition.

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