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Site-directed mutagenesis of the cGMP phosphodiesterase subunit from bovine rod outer segments: Role of separate amino acid residues in the interaction with catalytic subunits and transducin a subunit

Valery, M. Lipkin; Vladimir, A. Bondarenko; Vasily, E. Zagranichny; Lyudmila, N. Dobrynina; Khakim, G. Muradov; Mikhael Yu. Natochin

Biochimica et Biophysica Acta - Molecular Cell Research 1176(3): 250-256

1993

ISSN/ISBN: 0167-4889
DOI: 10.1016/0167-4889(93)90052-q
Accession: 062335863
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The recombinant and 21 mutant phosphodiesterase (PDE) γ subunit (PDEγ) genes were expressed by sequential transcription and translation in vitro. Inhibitory properties of these mutants and their interactions with Pde catalytic and transducin α subunits were studied. The interaction of the Pde γ subunit with the catalytic ones proceeds in two steps - primary binding and inhibition.

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Related References

Lipkin, V.M.; Bondarenko, V.A.; Zagranichny, V.E.; Dobrynina, L.N.; Muradov, K.G.; Natochin, M.Y. 1993: Site-directed mutagenesis of the cGMP phosphodiesterase γ subunit from bovine rod outer segments : role of separate amino acid residues in the interaction with catalytic subunits and transducin α subunit Biochimica et Biophysica Acta. Molecular cell Research 1176(3): 250-256
Lipkin, V.M.; Bondarenko, V.A.; Zagranichny, V.E.; Dobrynina, L.N.; Muradov, K.G.; Natochin MYu 1993: Site-directed mutagenesis of the cGMP phosphodiesterase gamma subunit from bovine rod outer segments: role of separate amino acid residues in the interaction with catalytic subunits and transducin alpha subunit Biochimica et Biophysica Acta 1176(3): 250-256
Lipkin, V.M.; Bondarenko, V.A.; Zagranichnyi, V.E.; Dobrynina, L.N.; Muradov, K., G.; Natochin, M.Y. 1994: Study of the role of separate amino acid residues in cGMP phosphodiesterase gamma subunit from bovine rod outer segments functioning by means of site-directed mutagenesis: Two-step mechanism of the gamma subunit interaction with phosphodiesterase catalytic complex Biologicheskie Membrany 11(3): 335-345
Lipkin, V.M.; Alekseev, A.M.; Bondarenko, V.A.; Muradov, K., G.; Obukhov, A.N.; Zagranichnyi, V.E. 1994: Site-directed mutagenesis of the cGMP phosphodiesterase inhibitory gamma subunit from bovine rod outer segments: A new hypothesis on the mechanisms of catalytic subunits inhibition by gamma subunit and holoenzyme activation by transducin Bioorganicheskaya Khimiya 20(8-9): 821-832
Bondarenko, V.A.; Desai, M.; Dua, S. 1997: Residues within the polycationic region of cGMP phosphodiesterase c subunit crucial for the interaction with transducin a subunit. Identification by endogenous ADP-ribosylation and site-directed mutagenesis Journal of Biological Chemistry 272: 856-864
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Lipkin, V.M.; Bondarenko, V.A.; Muradov, K.G.; Dobrynina, L.N.; Skiba, N.P.; Natochin, M.Y. 1992: Site-directed mutagenesis of the bovine rod outer segments cGMP phosphodiesterase gamma-subunit Sensornye Sistemy 6(3): 47-50
Bondarenko, V.A.; Matsumoto, H.; Yamazaki, A. 1997: Identification of amino acid residues in the polycationic region of gamma subunit of cGMP phosphodiesterase crucial for the interaction with transducin alpha subunit IOVS Investigative Ophthalmology and Visual Science 38(4 Part 1-2): S23
Skiba, N.P.; Artemyev, N.O.; Hamm, H.E. 1995: The carboxyl terminus of the c-subunit of rod cGMP phosphodiesterase contains distinct sites of interaction with the enzyme catalytic subunits and the a-subunit of transducin Journal of Biological Chemistry 270: 210-215
Skiba, N.P.; Artemyev, N.O.; Hamm, H.E. 1995: The carboxyl terminus of the gamma-subunit of rod cGMP phosphodiesterase contains distinct sites of interaction with the enzyme catalytic subunits and the alpha-subunit of transducin Journal of Biological Chemistry 270(22): 13210-13215
Arshavsky, V.Y.; Slepak, V.Z.; Zhu, Y.; Simon, M.I.; Bownds, M.D. 1994: Mechanism of transducin GTPase regulation by the gamma-subunit of cGMP phosphodiesterase from bovine rod outer segments Biophysical Journal 66(2 Part 2): A355
Natochin, M.Yu; Muradov, K.G.; Bondarenko, V.A.; Dobrynina, L.N.; Skiba, N.P.; Lipkin, V.M. 1991: Significance of individual amino acid residues of the inhibitory gamma subunit in its interaction with catalytic alpha and beta subunits of cgmp phosphodiesterase from the retinal rods Biologicheskie Membrany 8(4): 442-445
Artemyev, N.O.; Mills, J.S.; Thornburg, K.R. 1993: A site on transducin a-subunit of interaction with the polycationic region of cGMP phosphodiesterase inhibitory subunit Journal of Biological Chemistry 268: 611-615
Artemyev, N.O.; Mills, J.S.; Thornburg, K.R.; Knapp, D.R.; Schey, K.L.; Hamm, H.E. 1993: A site on transducin α-subunit of interaction with the polycationic region of cGMP phosphodiesterase inhibitory subunit Journal of Biological Chemistry 268(31): 23611-23615
Artemyev, N.O.; Mills, J.S.; Thornburg, K.R.; Knapp, D.R.; Schey, K.L.; Hamm, H.E. 1993: A site on transducin alpha-subunit of interaction with the polycationic region of cGMP phosphodiesterase inhibitory subunit Journal of Biological Chemistry 268(31): 23611-23615
Artemyev, N.O.; Mills, J.S.; Thornburg, K.R.; Knapp, D.R.; Schey, K.L.; Hamms, H.E. 1994: A site on transducin alpha-subunit of interaction with the polycationic region of cGMP-phosphodiesterase gamma-subunit Journal of Cellular Biochemistry Suppl 1994(18B): 305
Cunnick, J.M.; Hurt, D.; Oppert, B.; Sakamoto, K.; Takemoto, D.J. 1990: Binding of gamma subunit of retinal rod outer segment phosphodiesterase with both transducin and the catalytic subunits of phosphodiesterase Biochemical Journal 271(3): 721-728
Cunnick, J.M.; Hurt, D.; Oppert, B.; Sakamoto, K.; Takemoto, D.J. 1990: Binding of the gamma-subunit of retinal rod-outer-segment phosphodiesterase with both transducin and the catalytic subunits of phosphodiesterase Biochemical Journal 271(3): 721-727
Navon, S.E.; Fung, B.K.K. 1987: Characterization of transducin from bovine retinal rod outer segments: participation of the amino-terminal region of Tα in subunit interaction Journal of Biological Chemistry 262(32): 15746-15751