Site-directed mutagenesis of the cGMP phosphodiesterase subunit from bovine rod outer segments: Role of separate amino acid residues in the interaction with catalytic subunits and transducin a subunit
Valery, M. Lipkin; Vladimir, A. Bondarenko; Vasily, E. Zagranichny; Lyudmila, N. Dobrynina; Khakim, G. Muradov; Mikhael Yu. Natochin
Biochimica et Biophysica Acta - Molecular Cell Research 1176(3): 250-256
The recombinant and 21 mutant phosphodiesterase (PDE) γ subunit (PDEγ) genes were expressed by sequential transcription and translation in vitro. Inhibitory properties of these mutants and their interactions with Pde catalytic and transducin α subunits were studied. The interaction of the Pde γ subunit with the catalytic ones proceeds in two steps - primary binding and inhibition.