The 3-untranslated region of the mouse cholesterol 7a-hydroxylase mRNA contains elements responsive to post-transcriptional regulation by bile acids
AGELLON, B. Luis; CHEEMA, K. Sukhinder
Biochemical Journal 328(2): 393-399
ISSN/ISBN: 0264-6021 DOI: 10.1042/bj3280393
To investigate the importance of the 3'-untranslated region (UTR) of the mouse cholesterol 7alpha-hydroxylase (cyp7) mRNA in post-transcriptional regulation of expression of the cyp7 gene, chimaeric genes encoding mRNA containing the structural sequence of chloramphenicol acetyltransferase (CAT) linked to either the 3'-UTR of the mouse cyp7 mRNA or the SV40 early gene mRNA were constructed. The human cytomegalovirus (CMV) promoter was used to drive the expression of all the chimaeric genes.